Study on biogenic amines in various dry salted fish consumed in China

This study was carried out to investigate the biogenic amines（BAs）, physicochemical property and microorganisms in dry salted fish, a traditional aquatic food consumed in China. Forty three samples of dry salted fish were gathered from retail and wholesale markets and manufacturers, which had been produced in various regions in China. Cadaverine（CAD） and putrescine（PUT） were quantitatively the most common biogenic amines. About 14% of the samples exceeded the histamine content standards established by the FDA and/or EU. The highest histamine content was found in Silver pomfret（Pampus argenteus）(347.79 mg kg^-1). Five of forty three samples exceeded the acceptable content of TYR(100 mg kg^-1), and 23.26% of dried-salted fish contained high contents of biogenic amines(above 600 mg kg^-1). In addition, species, regions, pickling processes and drying methods made the physicochemical property, microorganisms and biogenic amines in dry salted fish to be different to some extents. The total plate count（TPC） was much higher than that of total halophilic bacteria in all samples. The biogenic amines, physicochemical property and microbiological counts exhibited large variations among samples. Furthermore, no significant correlation between biogenic amines and physicochemical property and TPC was observed. This study indicated that dry salted fish may still present healthy risk for BAs, depending on the processing methods, storage conditions among others.     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

Production and Certification of Synthetic Selenium Isotopic Reference Materials

Due to intensive research into selenium isotopes in recent years, the increasing requirement for reliable and comparable measurement results has created a strong demand for selenium isotopic certified reference materials (iCRM) that were previously not available. To address this, eleven selenium iCRMs were developed, including ten synthetic iCRMs (GBW 04447–GBW 04456) and one natural iCRM (GBW 04457). The synthetic iCRMs were prepared with ⁷⁶Se, ⁷⁸Se, ⁸⁰Se and ⁸²Se solutions and a natural selenium solution; the natural iCRM was prepared with highly pure selenium material. The property values of isotope ratios in these iCRMs were certified by calibrated mass spectrometry with a collision cell multi‐collector ICP‐MS. The mass discrimination effect of the instrument was corrected with corresponding ⁷⁸Se/⁷⁶Se isotope mixtures and ⁸²Se/⁷⁶Se isotope mixtures, which were gravimetrically prepared with purified, isotopically enriched selenium materials. Homogeneity and stability tests were performed, and no significant influences were found. The uncertainty of the property values of the iCRMs was evaluated according to the Guide to the Expression of Uncertainty in Measurement (GUM) of ISO/BIPM and ISO Guide 35. The δ^82/76Se value of GBW 04457 relative to NIST SRM 3149 was also calculated. These iCRMs are intended for use in calibration of instruments and evaluation of methods for the determination of selenium isotope ratios.     

基于恐龙足迹重建攀西地区白垩纪恐龙动物群

攀西地区没有白垩纪恐龙骨骼化石记录,对该区白垩纪恐龙动物群组合的认识尚属空白。1991年,攀西地区首次发现了恐龙足迹,此后陆续发现了8个足迹点。这些足迹点共发现9种不同的非鸟恐龙足迹(6种非鸟兽脚类、1种蜥脚类和2种鸟脚类足迹),以及翼龙和龟类足迹。共11种足迹形态类型由185道行迹(与孤立足迹)组成,可能代表着同等数量的造迹者。其中飞天山组的组合多样性最强,小坝组和雷打树组较弱。这个相对全面的足迹数据库,为该地区白垩纪四足类的古生态学普查提供了动物群的组成信息,这在该缺乏骨骼化石的地区显得尤为重要。     

Cannibalistic viruses in the aquatic environment: role of virophages in manipulating microbial communities

The ecological role of viruses in aquatic environments is gaining interest due to their abundance and overall diversity. Much focus has been on bacteriophages since they were found to play an important role in the diversification and sustainment at both the micro- and macro-scale. However, the discovery of virophages coexisting with giant viruses in a diverse set of eukaryotic hosts has recently gained attention. Virophages are small double-stranded DNA viruses found parasitizing giant viruses of eukaryotes. Since the discovery of the first virophage (Sputnik) many virophage signatures have been detected from a variety of environmental samples with specific infection cycles. In addition, these parasites display important roles in equilibrating microbial biomass, nutrient cycling and population dynamics. Moreover, virophage-induced evolution between giant viruses and their hosts have also been described. Considering the ongoing discovery of virophages and their dynamics in aquatic ecosystems, this review intends to provide an update of the virophage signatures identified to date, also tending to provide insight on the mechanisms of coinfection as well as their role as agents of biodiversity and nutrient cyclers in water environments.     

Effects of size and strain rate on the mechanical behaviors of rock specimens under uniaxial compression

Size and strain rate are two key factors that dramatically influence the estimation of rock mechanical behaviors. To better understand the effects of size and strain rate on measured rocks, rock specimens with six different sizes were tested at six different strain rates under uniaxial compression using the MTS 815 Rock Mechanics Test System. Having determined that the size and strain rate significantly affect the peak strain, peak stress, elastic modulus, acoustic emission (AE), and failure pattern of the rock specimens, the relation was established between the strength and the size and strain rate of red sandstone. And the variation was revealed among the size and strain rate, the AE, and the failure pattern. It turned out that the peak stress was negatively correlated with the rock size and was positively correlated with the strain rate. When the length to diameter ratio (L/D) of the rock specimen was less than 2.0, the AE appeared mildly. The AE quantities gradually increased before the peak stress, and then sharply decreased after the peak stress. The failure pattern of the rock specimen was relatively complicated, with a fracture plane appearing along the axial direction. Conical failure type was also presented. When the L/D ratio of the specimen was greater than 2.0, the AE characteristics of red sandstone showed the radical model. There were relatively few AE rings before the peak stress. But the AE rings increased suddenly and dramatically during the peak stress. The rock specimens primarily failed with a single shear plane. Moreover, with an increase in the strain rate, the AE activities were enhanced and the AE quantities increased. When the strain rate of the rock specimen was less than 5.0?×?10^?4/s, the rock specimen failed with a shear or tensile-shear pattern. And when the strain rate was greater than 5.0?×?10^?4/s, the rock specimen tended to fail in a conical pattern.