长牡蛎碱性磷酸酶的分离纯化及部分性质研究

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.     

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

热带大西洋年际和年代际变率的时空结构模拟

使用美国夏威夷大学发展的中等复杂程度海洋模式(IOM)在给定表面强迫条件下模拟了热带大西洋上层海洋年际和年代际变率的时空结构.利用NCEP的41a(1958～1998年)逐月平均表面资料作为强迫场,积分海洋模式41a作为控制试验,并利用模式分别做动量(风应力)通量和热量通量无异常变化的平行试验,与控制试验作比较.对3组试验模拟上层海洋变率状况的比较,并按年际和年代际时间尺度分别分析,揭示表面风应力和热通量异常对海表面温度和温跃层深度变化的影响,并比较了其影响的相对重要性.结果表明模式成功地模拟出了热带大西洋上层海洋的变率.模式模拟的海表面温度年际变化主要表现为弱ENSO型,年代际变化表现为南、北大西洋变化相反的偶极子型.在年际时间尺度上,热力强迫和动力强迫对海表温度变化都有贡献,其中赤道外海表面温度异常(SSTA)变化主要由热通量异常引起,而近赤道SSTA的变化主要由动量异常强迫引起.在年代际时间尺度上,热通量强迫的作用远比动量强迫重要.模式不仅能够模拟SST在年际和年代际时间尺度上的变率,还能够模拟温跃层深度在年际和年代际时间尺度上的变率.年际和年代际时间尺度上,温跃层深度的变率主要由动量异常决定,热通量异常强迫的贡献很小.     

Neural Network-Based Active Control for Offshore Platforms

1 .IntroductionWiththedevelopmentofoceantechnology ,moreandmoreextremelylargeandlongflexibleoff shoreplatformsusedforoilexplorationanddrillingoperationarebuiltinhostileoceanenvironments .Ingeneral,thiskindofplatformsisanonlineardistributedparametersystemanditsnaturalfrequencyfallsclosertothedominantwavefrequencieswiththeincreaseofwaterdepth .Besides ,itsstructureisverycomplexandtheexternalwaveforceontheplatformisuncertain .Thus ,theseplatformsarepronetoexcessivewave inducedoscillationsunderbot…     

基于非期望产出的中国滨海旅游生态效率评价——以我国沿海城市为例

旅游生态效率是反映旅游经济与旅游环境双向效应(bi-effect)的重要判断指标。本文基于非期望产出视角,采用超效率SBM模型,评价了我国东部沿海18个主要滨海旅游城市的旅游生态效率,并分析了其时空演变特征。研究结果表明:(1)中国东部沿海18市滨海旅游生态效率整体呈中等水平,平均值处于0.727～0.894,阶段特征明显,呈现前期平稳发展,中期下降,后期回升的发展趋势;(2)各城市滨海旅游生态效率水平差异显著,分为下行风险型,持续成长型,稳定型和波动型4种发展类型。平均效率值最高为天津,最低为三亚;(3)分解效率均值中纯技术效率较高,规模效率次之,技术进步是影响综合效率的主要因素;(4)投入冗余和非期望产出冗余已经成为阻碍滨海旅游生态效率进步的关键因素。     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

基于GIS的柘林湾生态信息系统

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．     

A STUDY OF THE DISTRIBUTION OF RECENT OSTRACOD FAUNAS FROM COASTAL AREAS OF THE EAST CHINA AND YELLOW SEAS

A total of 91 species belonging to 57 genera were recovered from the coastal areas of the north East China Sea ana the south Yellow Sea, of which, 42 species of 30 genera were collected alive. The biocoenoses of estuaries, supralittoral and eulittoral zones are dominated by orackish and euryhaline species, of which Albileberis sheyangensis, A. sinensis, Leptocythere ventriclivosa, Loxoconcha ocellata, Sinocytheridea latiotata and S. longa are ubiquitous; Propontocypris euryhalina, Spinileberis furuyaensis and S. pulchra are restricted to the supralittoral zone; Neomonoceratina crispata and Pontocythere lit-toralis are only present in the eulittoral zone. The community structure of these biocoenoses is generally marked by low snecies diversity (H(S) and 5) and high incidence and dominance. Among the environmental factors investigated, it was found that salinity was the most important in controlling the distribution of living ostracods, and high-energy hydrodynamic condition was the main cause of the grea     

A three-dimentional model of the northeast East China Sea, with application to the M2 current field

A simple three-dimensional tidal model is used to examine the M2 tidal current distribution in a northeastern part of the East China Sea, especially the vertical variation of the current in the region. Computed M2 current is compared with observations available and found to be in good agreement.Main features of the calculating method in this study are: (1) Vertical variation of the tidal current is taken as a funetion of the depth-mean velocity: (2) the method is applicable to a variety of the vertical eddy viscosities; (3) it has a fine vertical resolution, especially near the sea bootom. So, this method not only enables us to get a steady state solution easily but also depicts effects of the friction on the vertical variation of the current much better.     

大西洋中脊TAG热液活动区中热液沉积物的稀土元素地球？…

用ＩＣＰ－ＭＳ对ＴＡＧ热液活动区表层热液沉积物中６个块状硫化物样品进行了稀土元素分析。所有样品均表现出正Ｅｕ异常和ＬＲＥＥ相对富集的球粒陨石标准化配分模式,热液沉积物样品中稀土元素组成的变化是由于海底热液循环体系中热液流体和涨水不同程度混合作用的结果,