氨氮胁迫对中华绒螯蟹(Eriocheir sinensis) 免疫功能的影响

在实验室条件下构建不同氨氮浓度环境和养殖时间的组间差异,研究氨氮胁迫对中华绒螯蟹免疫功能的影响。结果表明,中华绒螯蟹在NH 4-N3.0mg/L10天或1.0mg/L20天时,其血淋巴血细胞密度极显著地低于对照组(P<0.01)。NH4 -N2.0mg/L和3.0mg/L无论10天或20天,血细胞吞噬百分率均分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N3.0mg/L10天或2.0mg/L20天,血细胞吞噬指数显著低于对照组(P<0.05)。NH4 -N1.0mg/L和2.0mg/L分别20天时,血清溶菌酶(LSZ)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。NH4 -N1.0—5.0mg/L10天时,血清酚氧化酶(PO)活力均极显著(P<0.01)下降;但NH4 -N1.0—4.0mg/L20天时,血清PO活力水平均比10天时有所上升。NH4 -N3.0—4.0mg/L10天或1.0—2.0mg/L20天,以及5.0mg/L10天或3.0—5.0mg/L20天时,超氧化物歧化酶(SOD)活力分别为显著(P<0.05)和极显著(P<0.01)地低于对照组。上述结果表明,随着氨氮胁迫浓度的增加和胁迫时间的延长,会引起中华绒螯蟹血细胞数量、血细胞吞噬能力、溶菌酶活力、酚氧化酶活力和超氧化物歧化酶活力等的逐渐下降,使机体非特异性免疫防御系统遭到损伤,同时机体清除自由基的能力下降,机体细胞和组织受到伤害甚至出现死亡。     

Numerical Simulation of Dynamic Response of Supple Nets

1 .Introduction Owing to the decline of capture fisheries andsaturation of traditional inshorefarms ,sea cage cul-ture as a newtype of aquaculture is developingrapidlyin China .However ,cage culture is a highriskoperation.The practice is vulnerable to natural hazards ,such as strong tides ,storms and typhoons .So,it is necessaryto assess the behavior of a sea cage exposedto waves and current before the designandinstallation of the structure . Computer simulation,due to its many advantages ,ha…     

Comparison of Drags on Fish Cages of Different Shapes

1 .Introduction Commercial fishfarming in net cages is becoming widespread around the world as an importantsource of food.In PRChina ,mostfishfarmingtakes placeincalmcoastal areas .Fishcages work wellat these sites becausethe environmental forces are usua…     

Molecular phylogenetic analysis of sulfate-reducing bacteria from deep sediment layers of the tropical West Pacific warm pool

1IntroductionThe tropical West Pacific warm pool(TWP-WP),which spans an area roughly between10°Nto10°S of the equator from Indonesia to the dateline,has the world’s warmest sea surface temperature ofbeing greater than29℃.With the increase of recog-niz…     

盐度调控对花鲈(Lateolabrax maculatus)生长相关基因表达的影响

生长激素受体(GHR)作为GH/IGF轴的中心环节,在内分泌调控中发挥重要作用。本实验采用c DNA末端快速扩增法(RACE)技术克隆出花鲈GHR1和GHR2的c DNA全长序列。急性低盐度调控实验设为海水组、半海水组和淡水组。测定了急性低盐度调控24、48、96、144、192h后,花鲈肝脏中GHRs、IGF-1及垂体GH表达情况。结果表明,GHR1 c DNA全长序列2436bp,编码637个氨基酸;GHR2 c DNA全长序列2940bp,编码582个氨基酸。GHR1与GHR2由信号肽、胞外区、跨膜区、胞内区组成,且结构存在差异。脑、肾、鳃中GHR1表达明显高于GHR2;而在肌肉、垂体、肝脏、盲肠、胸腺、心脏中,GHR2表达明显高于GHR1。24h时,各组GHR1表达不变,GHR2、GH、IGF-1显著下降。之后,相对于海水组,淡水组和半海水组GHRs和IGF-1表达升高,而GH下降,GH与GHR负相关。据结果推测,花鲈GHR2可能为SL受体,GH/IGF轴参与低渗调控可能是通过增加GHRs,进而激活下游IGF-1表达而实现。     

水下溢油数值模拟研究

基于Lagrange积分法和Lagrange粒子追踪法建立了一个水下溢油数值模型。该溢油模型由两个子模型组成:羽流动力模型和对流扩散模型,其中羽流动力模型用以模拟溢油的喷发阶段和浮力羽流阶段;对流扩散模型用以模拟溢油的对流扩散阶段。通过数值实验,结合实验室水槽实验和水下溢油现场实验的观测资料进行模型验证。实验结果表明,模拟结果与观测资料一致性较好,从而验证了本文溢油模型的合理性和准确性;羽流动力模型为对流扩散模型提供源,海流、海水的垂向密度结构和油滴的直径分布是影响溢油在对流扩散阶段运动和分布的主要因素。     

Analysis of A Porous and Flexible Cylinder in Waves

The hydrodynamic response of a porous flexible circular-cylinder in regular waves was analytically studied. To simplify the problem, the cover and the bottom of the cylinder were ignored. Small amplitude water wave theory and structural responses were assumed. The velocity potentials were solved using the Fourier-Bessel series expansion method and the least squares approximation method. The convergence of the series was numerically tested to determine the number of terms in the series expansion. Two types of installations were considered for deformation, hydrodynamic forces, structural flexibility, drafts, and porosity. The present study represented a preliminary step in the study of the fish cage.     

南极半岛东北部海域表层沉积物稀土元素特征及物源指示意义

通过对南极半岛东北部海域36个表层沉积物样品的稀土元素分析发现:(1)研究区表层沉积物中稀土元素总量(REE)的分布范围为(45.47~183.60)×10-6,平均值为91.36×10-6;稀土元素含量的高低与铝硅酸盐黏土成正比,与生物硅含量成反比;(2)大致以象岛至南极半岛北部的茹安维尔岛为界,研究区西部的布兰斯菲尔德海峡及南设得兰群岛北部岛架-岛坡沉积物稀土含量较低,稀土元素页岩标准化模式与长城站周边土壤/湖泊沉积物、火山岩相一致,表现为重稀土相对富集,铕正异常明显,Sm/Nd分子比值大,推断该区沉积物主要来源于南设德兰群岛中-新生代火山岩分布区与现代火山活动区;(3)研究区东部鲍威尔海盆、南奥克尼群岛岛架和南斯科舍海脊区沉积物稀土元素的页岩标准化配分模式表现为平坦型,轻稀土明显抬升,铕正异常不显著,Sm/Nd分子比值小,稀土元素含量受沉积物中黏土组分含量、硅质生物组分等制约,推断沉积物陆源碎屑主要来源于威德尔海西部和南部,局部受南奥尼克群岛、南斯科舍海脊和南设德兰群岛物质的影响,沉积物的搬运主要受冰筏和海流制约。     

3种海洋微藻微胶囊培养的初步研究

用12mg/mL羧甲基纤维素钠(CMC)、10mg/mL海藻酸钠(SA)和0.5mmol/mL CaCl2制备亚心形扁藻、绿色杜氏藻和球等鞭金藻8701的中空海藻酸钙微胶囊,3种微藻在微胶囊内生长表现突出,最高细胞密度亚心形扁藻为70.4×106/mL,绿色杜氏藻为100.9×106/mL,球等鞭金藻8701为264.1×106/mL,分别是悬浮培养微藻最高密度的35倍、27倍和84倍,结果说明微胶囊化培养是微藻高密度培养的有效方式。