急性低氧胁迫对卵形鲳鲹选育群体血液生化指标的影响

本文研究了水环境急性低氧胁迫对卵形鲳鲹Trachinotus ovatus选育群体血液生化指标的影响。实验设计了急性低氧试验组和对照组,每组3个平行。结果显示,急性低氧胁迫后选育群体的血清离子含量与对照组相比都有不同程度的升高,其中钠、氯、钙浓度与对照组相比差异显著(p0.05),铁浓度极显著高于对照组(p0.01);血清蛋白、尿酸、肌酐、血脂、血糖等指标的差异较大,其中尿酸、肌酐极显著高于对照组(p0.01),总蛋白、总胆固醇显著低于对照组(p0.05);血清中肌酸激酶含量极显著高于对照组(p0.01),肌酸激酶同工酶显著高于对照组(p0.05),γ-谷氨酰转肽酶含量显著低于对照组(p0.05);其他指标均有不同程度的升高或降低,但差异不显著。研究表明,急性低氧胁迫造成卵形鲳鲹鳃、肾、心脏和肝脏损伤,免疫力下降。     

基于卫星高度计的北极海冰厚度变化研究

A modified algorithm taking into account the first year(FY) and multiyear(MY) ice densities is used to derive a sea ice thickness from freeboard measurements acquired by satellite altimetry ICESat(2003–2008). Estimates agree with various independent in situ measurements within 0.21 m. Both the fall and winter campaigns see a dramatic extent retreat of thicker MY ice that survives at least one summer melting season. There were strong seasonal and interannual variabilities with regard to the mean thickness. Seasonal increases of 0.53 m for FY the ice and 0.29 m for the MY ice between the autumn and the winter ICESat campaigns, roughly 4–5 month separation, were found. Interannually, the significant MY ice thickness declines over the consecutive four ICESat winter campaigns(2005–2008) leads to a pronounced thickness drop of 0.8 m in MY sea ice zones. No clear trend was identified from the averaged thickness of thinner, FY ice that emerges in autumn and winter and melts in summer. Uncertainty estimates for our calculated thickness, caused by the standard deviations of multiple input parameters including freeboard, ice density, snow density, snow depth, show large errors more than 0.5 m in thicker MY ice zones and relatively small standard deviations under 0.5 m elsewhere. Moreover, a sensitivity analysis is implemented to determine the separate impact on the thickness estimate in the dependence of an individual input variable as mentioned above. The results show systematic bias of the estimated ice thickness appears to be mainly caused by the variations of freeboard as well as the ice density whereas the snow density and depth brings about relatively insignificant errors.     

高效非损伤性扇贝DN A提取方法的建立及效果评价

本研究建立了一种高效、经济的非损伤性扇贝DNA提取方法,在不影响扇贝个体生存状态的前提下,采用擦拭法和室温裂解相结合的方式在20 min内即可获得个体基因组DNA。以虾夷扇贝(Patinopecten yessoensis)、栉孔扇贝(Chlamys farreri)和海湾扇贝(Argopecten irradians)为实验对象,研究了不同擦拭材料(棉签、滤纸)和不同擦拭部位(外套膜、鳃丝、内脏团)的核酸提取效果,评估了本方法在贝类基因分型领域应用的可行性。研究表明,用棉签、滤纸2种材料擦拭扇贝的鳃丝、内脏团或外套膜组织均能获得主带清晰完整的基因组DNA,且不影响扇贝的存活状态。在3种扇贝中采用棉签擦拭法的DNA得率均高于滤纸法,以这2种方式获得的DNA为模板进行SSR和SNP标记分析,能获得均一稳定的扩增条带,SSR标记分型结果准确可靠。本研究建立了简便、快速进行扇贝基因型分析的非损伤性DNA提取方法,为贝类全基因组选育和珍稀贝类资源的种质保护开发提供了新的技术手段。     

北太平洋海表面高度(SSH) 与风应力变化的关系

利用1958-2008年的北太平洋海表面高度和风应力资料,并与ENSO和PDO指数进行相关分析.结果发现,风应力及其经向分量主要通过季节振动影响海表面高度(SSH)的年周期变化,纬向风应力主要通过多年振动影响SSH的ENSO和PDO周期.纬向风应力和SSH均以黑潮延伸体主轴为界,两侧呈现出相反的升降趋势,SSH为北降南升,纬向风应力南降北升.风应力和SSH升降趋势相同,均表现为“上升—下降—上升”的变化特征.在地形变化剧烈、等深线南北分布的海区,西风增强会导致SSH升高,且西侧升高较为明显.北风增强将导致北太平洋西岸SSH升高,东岸SSH降低.     

应用高分辨率熔解曲线(HRM)开发大菱鲆(Scophthalmus maximus)SNP标记的研究

利用Vector NTI Advance 11软件分析了NCBI数据库中大菱鲆的12428条EST序列,筛出候选SNP位点;应用高分辨率熔解曲线(HRM)对大菱鲆不同性状群体进行基因分型。结果表明,522个重叠群中共筛出160多个候选SNP位点;设计56对引物用于实验,能扩增出目的片段的引物有37条,合45个位点,成功率为66.1%;设计探针,能与候选SNP位点杂交的探针有13条,杂交率为28.9%。本实验中能成功进行基因分型的位点共有8个,占杂交位点的61.5%,其中有6个为碱基替换型位点,即G-A和C-T各占3个,2个为碱基颠换型位点,即T-G和A-T各占1个。     

MARINE BIOLOGICAL CHORUSES OBSERVED IN XIAMEN HARBOUR

Marine biological choruses observed in Xiamen harbour from August 1981 to October 1982 lasted for a few hours each time during the neap tide in spring, summer and autumn, in both the evening and the morning, with molt energy at frequencies between 700 Hz and 1.6 kHz and spectral peaks mainly at 800 Hz, 1 kHz and 1.25 kHz. During choruses, noise spectrum levels rose up to 46 dB higher than the background noise in the sea Mate 0. The statistics of occurrence of the evening choruses indicate that they might be fairly widespread in and near shallow waters of the region. Sources of such choruses are likely to be attributed to Johnnius belengerii and Collichthys lucidut of the family Sciaenidae.     

西伯利亚北极河流有机碳输出特征及影响要素

河流有机碳输出是北极碳循环的重要组分,对气候变化十分敏感。本文利用ArcticGRO的径流及有机碳数据,通过数理统计分析等方法,对2004—2017年间俄罗斯西伯利亚地区的鄂毕河、叶尼塞河和勒拿河有机碳输出进行研究。结果显示：西伯利亚3大河流的年均有机碳输出总量约23 Tg,其中溶解有机碳（DOC）输出约18.55 Tg,占北极地区50%以上,接近颗粒有机碳（POC）输出的4倍。2009—2017年间的年均DOC输出量较1999—2008年存在较大差异,鄂毕河增加18%,叶尼塞河下降13%,而勒拿河增加了近70%。春、夏两季有机碳输出总量占全年85%以上,春季为有机碳输出高峰期,而在鄂毕河流域春、夏季贡献率相当。各流域河流有机碳输出特征不同且具有季节性变化,主要受径流、冻土及人类活动等的影响。其中,DOC输出总量在年际与季节性变化特征上均与径流变化呈显著正相关,随径流增加DOC浓度也相应增大;而多年冻土也因类型及分布差异对河流DOC与POC具有不同程度的影响。研究气候变化下的北极河流有机碳输出特征及影响要素,有助于深入理解北极河流有机碳输出对气候及环境变化的综合响应,为揭示气候变化下的北极碳循环过程奠定基础。     

Urban planning construction land standard and its revision based on climate and topography in China

Urban planning construction land standard is the technical specification for scientifically allocating various types of urban construction land, and it is the basis for drawing up and revising the overall urban planning scheme. Considering China's current urban planning construction land standard, many problems exist, such as the gap in the land use control threshold, the lack of regional differences in the climate revision, and failing to consider the topographic factors. To resolve these problems, this study proposed a step-by-step process framework and quantitative calculation method for the establishment and revision of standards in accordance with the principle of Total-Structure control. By setting the conditions, a universal basic standard for construction land was established. Quantitative analysis was then conducted on the relationship between the basic standard and the selected key indicators, such as urban population size, sunshine spacing coefficient, the width of river valleys or inter-montane basins, and terrain slope, among others. Finally, revised standards were formed for climate conditions, topography, and geomorphologic conditions, which were matched with the basic standards. The key results are three-fold:(1) The per capita construction land standard of 95 m~2/person can be used as the total indicator of China's urban planning basic standard, and the corresponding per capita single construction land comprises 32.50% of residential land, 7.42% of public management and public service land, 22.50% of industrial land, 17.50% of transportation facilities, 12.50% of green space, and 7.58% of other land-use types. The results of the revision of the urban population size indicate that the difference in population size has little effect on the total amount of per capita construction land.(2) The climate revision results of per capita residential land and per capita construction land in major cities reveal that the revised climate value varies greatly between north and south China. The revised climate values of the per capita area of construction land vary by latitude as follows: the value at 20°N is 93 m~2/person, the value at 30°N is 97 m~2/person, the value at 40°N is 103 m~2/person, and the value at 50°N is 115 m~2/person. The basic standard land value of 95 m~2/person is generally distributed across the Xiamen-Guilin-Kunming line.(3) The cities located in mountainous areas, hilly valleys, or inter-montane basins can reduce the allocation of community parks and comprehensive parks when the average width of an existing river valley or inter-montane basin is less than 2 km. When the average width of the valley or inter-montane basin is between 2 km to 4 km, the allocation of the comprehensive parks can be reduced. The revised results of per capita sloping construction land reveal that the terrain slope greatly affects the revised value of per capita construction land. Specifically, the revised value at 3° is 3.68% higher than the basic standard value, and the increase rates at 8°, 15°, and 25° are 11.25%, 26.49%, and 68.47%, respectively.     

PM2.5污染对中国人口死亡率的影响——基于346个城市面板数据的实证分析

中国生态文明建设和“健康中国”战略强调切实治理影响人口健康的环境问题,建设健康人居环境。论文基于2000年和2010年中国人口普查资料以及2005年和2015年各省级行政单元1%人口抽样调查等数据资料,论文使用探索性空间分析方法刻画中国城市人口死亡率的时空变化特征,并采用空间回归方法,揭示城市PM_2.5的平均浓度对人口死亡率的影响及其空间溢出效应,以及社会经济因素对PM_2.5—人口死亡率关联的调节效应。结果表明：① 中国城市人口死亡率的空间分布特征呈现明显的异质性,高死亡率地区早期集聚分布于西南地区,2005年后在西南地区、华北地区、华东地区和华中地区呈现逐渐集聚分布态势。低死亡率地区长期集中分布于西北地区、东北地区、长三角地区、珠三角地区和京津两市。② 人口死亡率的分布存在空间关联性,高—高类型地区早期集中分布于西南地区,后期向东扩展;低—低类型地区主要分布于北疆、内蒙古西部和广东省及其周边地区。③ 城市PM_2.5浓度对人口死亡率具有显著的正向影响,并且对邻近地区的人口死亡率具有显著的空间溢出效应。④ 中国城市PM_2.5浓度对人口死亡率的影响存在学历差异和城乡差异,地区高学历人群集聚可降低PM_2.5的健康风险,城镇化发展进程缓慢则会加重PM_2.5的健康风险。研究旨在为防范空气污染暴露导致的健康风险、建设健康人居环境提供科学依据。     

北极涛动低空大气环流特征及其与太阳活动的联系

通过北极涛动AO正负位相时期北半球1000 hPa月平均位势高度、位势高度距平和气温月距平图对比分析可知,北极区域异常增暖时期对应着AO负位相时期,而北极区域异常偏冷时期对应着AO正位相时期,说明北极区域气温异常变化是决定AO异常变化的重要因子.逐次滤波法分析可知,冬季1月北极涛动现象表现出十分清楚的与太阳活动密切联系的准110a世纪周期和准22a年代际周期,具体表现为：（1）冬季1月北极涛动现象具有十分清楚的与太阳活动密切联系的准110a世纪周期.准110a世纪周期对于北极涛动指数的方差贡献率达到44.4%,是冬季1月北极涛动现象最显著的世纪际变化特征.（2）谱分析结果表明,滤除准110a世纪周期变化以后的1月北极涛动指数具有显著的22a周期,其方差贡献率达到18.5%,乃仅次于准110a世纪周期之后北极涛动指数年代际变化重要特征.对比分析表明,太阳活动尤其是太阳磁场磁性指数变化与1月北极涛动22a周期变化呈密切的反相关关系,二者变化趋势基本相反,即多数情况当太阳磁性指数MI由最低值转为上升以后都可引起北极涛动AO由最高值转为下降;当太阳磁性指数MI由最高值转为下降以后都可引起北极涛动AO由最低值转为上升.综上所述,北极涛动的准110a世纪周期变化、22a年代际周期变化对于北极涛动方差贡献率达到62.9%,标志着太阳活动是北极涛动的重要驱动因子.