中国近海外来囊藻(Colpomenia peregrina)种群遗传多样性研究

外来囊藻(Colpomeniaperegrina)是一种在潮间带分布广泛的褐藻,它能附着在牡蛎壳上进行跨海区扩散,因而是研究生物多样性变动和生物地理格局形成的良好模型。外来囊藻在中国沿海虽有分布,但关于其种群遗传多样性和分化研究的报道尚为空白。本研究采集了中国近海13个外来囊藻种群样本。通过扩增301条线粒体cox3序列,发现了26个单倍型;通过重建单倍型网络图和基于最大似然法与贝叶斯法重建系统进化树,发现外来囊藻种内遗传多样性较高, 13个种群划分为3个遗传世系,其中浙江南麂列岛的7个种群组成遗传世系A,辽宁和山东的样本则分化为遗传世系B和C。中国近海外来囊藻的这种种群遗传结构可能源于更新世末次盛冰期黄渤海、东海边缘海的环境变化和海平面的大幅下降。遗传世系A具有的较高单倍型多样性,其原因可能是南麂列岛独特的地理位置和潮间带环境的共同作用。     

ICESat-2激光卫星与光学遥感影像融合水深测量

针对浅海测深的数据特点和应用需求,以我国南海甘泉岛为例,研究了利用ICESat-2(Ice,Cloud,and Land Elevation Satellite-2)激光卫星数据和光学遥感影像开展主被动融合水深测量的方法。首先通过信号点提取、水面/水底识别、水下点折射改正等步骤处理ICESat-2数据,获得水深值,随后以激光点作为控制,计算光学水深反演模型参数,最后由点及面地获取大范围高精度水深。实验表明,甘泉岛区域主被动融合测深中误差优于1.30m,基于激光卫星数据的主被动融合测深方法能够为浅水水深测量提供新手段。     

AUV矢量推进水动力计算及水平回转操纵性试验

针对矢量推进模式下的 AUV 水动力性能和操纵性能,开展了 AUV 矢量推进水动力 CFD 计算, 对矢量推进水平回转操纵性进行了评估,加工了矢量推进样机,并进行湖上操纵性验证。理论计算和试验数据表明：矢量推进具备极高的低速操纵性,该研究为矢量推进在 AUV 上的应用提供了理论和技术基础。     

雅浦-马里亚纳海沟附近海域的精细地貌特征研究

利用西太平洋地区的雅浦-马里亚纳海沟附近海域的多波束水深数据,获得了9.8°N—13.2°N、132.2°E—145°E矩形区域内的高精度水深地形图。通过对精细地貌进行研究发现,马里亚纳海沟南部的洋坡上发育有大范围的地垒、地堑型地貌,雅浦海沟北部存在明显的分段性特征,菲律宾海盆和帕里西维拉海盆内存在不同展布方向的大规模脊-槽地貌。研究结果表明此处复杂的地形地貌特征主要受板块运动的控制。最后结合已有研究成果,对该区域的地形地貌进行了分类。     

浙江苍南近岸海域海床冲淤过程研究

近岸海床冲淤变化直接反映海区地貌稳定状态,由此影响海区物质迁移及水运资源可持续利用。基于此,本研究通过选择苍南海域不同时期的海图资料,基于ArcGIS平台探讨该海域多尺度海床冲淤过程,为研究区海岸港工建筑规划与评估提供理论指导。主要研究结果包括：苍南海域海床冲淤变化大致分为四个阶段,即1931—1970年大幅淤积,海床净淤积量达到169.47×10⁶m³,淤积强度为5.18 cm/a;1971—2005年海床淤积幅度减弱,海床净淤积量为12.24×10⁶m³,淤积强度为0.41 cm/a;2006—2009年海床由淤转冲,海床净冲刷量为14.70×10⁶m³,冲刷强度为3.60 cm/a;2010—2017年海床持续冲刷,海床净冲刷量为10.17×10⁶m³,冲刷强度为1.33 cm/a。除1931—1970年10 m等深线向海大幅扩张以及1971—2005年10 m等深线有部分向海扩张外,1971—2017年5 m与10 m等深线普遍向陆后退。2006—2017年,位于北关港内的2 m等深线也向陆后退。冲淤结果显示,苍南海域海床未来可能呈现弱侵蚀态势。其中,风暴潮频发导致海床经常性失稳加之长江入海泥沙的减少导致该区域泥沙补给不足,二者共同作用可能是该区域海床由淤积逐渐转为侵蚀的主导因素。     

海水温度对衰亡期浒苔释放生源硫影响的模拟研究

为研究浒苔释放生源硫的特征,本文对采集于黄海绿潮中期和末期的浒苔进行了实验室模拟培养,探讨了不同温度对衰亡期浒苔释放生源硫化物的影响。实验结果表明,在10~25℃温度范围内,温度升高能够加速浒苔的衰亡。二甲基硫（DMS）的平均释放速率范围为2.79~150.70 nmol/（L·g·d）,二甲基硫基丙酸内盐（DMSP）的平均释放速率范围为2.16~113.26 nmol/（L·g·d）。温度升高能够使DMS和DMSP的释放速率加快,释放量增加,DMS最大平均释放速率在25℃条件下比10℃条件下升高了约60%,培养液中DMS浓度升高了2~3倍。采集于绿潮末期的浒苔培养液中的DMS和DMSP和采集于绿潮中期的浒苔相比,浓度有所增加,采集于浒苔绿潮末期浒苔培养液中DMS的最高平均浓度为418.41 nmol/L,约为中期的4倍;DMSP的最高平均浓度为316.14 nmol/L,是中期的3倍。浒苔绿潮的爆发会对水体中的硫体系循环产生影响,进而影响该海域生态环境。     

利用扩增vvhA基因的环介导等温扩增技术快速，特异而敏感地检测创伤弧菌

创伤弧菌是一种可感染人类的河口病原菌。建立快速,特异而敏感的检测方法,有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物）,采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示,本方法的最适扩增温度是63℃,反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出,也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性,其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍,同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较,针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。     

Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris

The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish.     

Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene

Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification(LAMP) technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.     

Dynamic Performance Investigation of A Spar-Type Floating Wind Turbine Under Different Sea Conditions

Both numerical calculation and model test are important techniques to study and forecast the dynamic responses of the floating offshore wind turbine (FOWT). However, both the methods have their own limitations at present. In this study, the dynamic responses of a 5 MW OC3 spar-type floating wind turbine designed for a water depth of 200 m are numerically investigated and validated by a 1:50 scaled model test. Moreover, the discrepancies between the numerical calculations and model tests are obtained and discussed. According to the discussions, it is found that the surge and pitch are coupled with the mooring tensions, but the heave is independent of them. Surge and pitch are mainly induced by wave under wind wave conditions. Wind and current will induce the low-frequency average responses, while wave will induce the fluctuation ranges of the responses. In addition, wave will induce the wavefrequency responses but wind and current will restrain the ranges of the responses.