急性高温胁迫对虹鳟二倍体和三倍体幼鱼hsps基因表达的影响

本文以二倍体和三倍体虹鳟(Oncorhynchus mykiss)幼鱼为实验对象,探究急性高温胁迫下,不同倍性虹鳟热休克蛋白家族(hsps)基因表达水平。选取二倍体和三倍体虹鳟幼鱼,分别进行常温处理(14℃)和高温胁迫(22℃),并在高温胁迫48h后,将虹鳟置于常温(14℃)恢复48h。分别在实验开始的0,12,24,48,72和96h进行取样,测定实验虹鳟肝脏和肌肉组织中的hsp70s(hsp70a,hsp70b),hsp90s (hsp90a1a,hsp90a2a,hsp90a1b,hsp90a2a,hsp90b1,hsp90b2)mRNA水平。结果表明:hsp70s和hsp90s的各个亚型均能对热胁迫做出反应,并具有应答幅度的组织特异性和时间特异性。在胁迫6和24h后,上述基因mRNA水平达到最高,随后降低。三倍体幼鱼肝脏和肌肉hsps mRNA表达水平的峰值显著高于二倍体幼鱼。研究结果显示,三倍体虹鳟对高温环境更为敏感,需要消耗更多的能量用于适应高温环境。     

人工冻土观测的冻点对应的土壤温度研究

基于2018年12月至2020年3月喀左、沈阳、辽阳、满洲里4个国家级地面气象站人工冻土器与测温式冻土自动观测仪观测的资料,对人工冻土观测获得的冻点与测温式冻土自动观测仪获得的相应深度的温度进行对比分析.结果表明:人工冻土器获取的冻点对应的土壤温度与0℃总体一致,又不完全重合;0—35 cm深度范围,冻点对应的温度变化...     

Effects of winds,tides and storm surges on ocean surface waves in the Sea of Japan

Ocean surface waves are strongly forced by high wind conditions associated with winter storms in the Sea of Japan. They are also modulated by tides and storm surges. The effects of the variability in surface wind forcing, tides and storm surges on the waves are investigated using a wave model, a high-resolution atmospheric mesoscale model and a hydrodynamic ocean circulation model. Five month-long wave model simulations are inducted to examine the sensitivity of ocean waves to various wind forcing fields, tides and storm surges during January 1997. Compared with observed mean wave parameters, results indicate that the high frequency variability in the surface wind filed has very great effect on wave simulation. Tides and storm surges have a significant impact on the waves in nearshores of the Tsushima-kaihyō, but not for other regions in the Sea of Japan. High spatial and temporal resolution and good quality surface wind products will be crucial for the prediction of surface waves in the JES and other marginal seas, especially near the coastal regions.     

Distribution of the nonlinear random ocean wave period

Because of the intrinsic difficulty in determining distributions for wave periods, previous studies on wave period distribution models have not taken nonlinearity into account and have not performed well in terms of describing and statistically analyzing the probability density distribution of ocean waves. In this study, a statistical model of random waves is developed using Stokes wave theory of water wave dynamics. In addition, a new nonlinear probability distribution function for the wave period is presented with the parameters of spectral density width and nonlinear wave steepness, which is more reasonable as a physical mechanism. The magnitude of wave steepness determines the intensity of the nonlinear effect, while the spectral width only changes the energy distribution. The wave steepness is found to be an important parameter in terms of not only dynamics but also statistics. The value of wave steepness reflects the degree that the wave period distribution skews from the Cauchy distribution, and it also describes the variation in the distribution function, which resembles that of the wave surface elevation distribution and wave height distribution. We found that the distribution curves skew leftward and upward as the wave steepness increases. The wave period observations for the SZFII-1 buoy, made off the coast of Weihai (37°27.6′ N, 122°15.1′ E), China, are used to verify the new distribution. The coefficient of the correlation between the new distribution and the buoy data at different spectral widths (ν=0.3−0.5) is within the range of 0.968 6 to 0.991 7. In addition, the Longuet-Higgins (1975) and Sun (1988) distributions and the new distribution presented in this work are compared. The validations and comparisons indicate that the new nonlinear probability density distribution fits the buoy measurements better than the Longuet-Higgins and Sun distributions do. We believe that adoption of the new wave period distribution would improve traditional statistical wave theory.     

基于部件可靠度和多阶段动态库存的备件管理决策

基于部件可靠度和多阶段动态库存理论,分析了备件需求与库存管理。提出了基于部件可靠度的动态需求模型;以部件的寿命服从指数分布为例,结合实际生产中备件的使用情况,通过确定备件储备的时间点,动态确定各周期备件的需求量。在确定备件动态需求量的基础上,采用多阶段动态库存理论确定了订货次数和每次订货数量,使库存量更符合实际和趋于合理。通过算例分析,给出了最小库存费用,表明基于部件可靠度和多阶段动态库存的备件管理具有一定的实际意义。     

岩石圈热-流变结构的研究方法和发展动向

岩石圈并不是经典板块理论认为的整体的刚性圈层,而是在不同大地构造位置及不同深度具有不同的流变性质。在介绍岩石圈热一流变结构的计算方法以及不同大地构造位置的岩石圈流变学分层特征的基础上,阐述了岩石圈热一流变结构所反映的地球动力学信息,并对我国在该领域的研究进展做出总结,认为未来需要加强深部结构和地热学的研究,尤其是我国海域此方面的研究。     

.Net环境下空间数据缓存方案的设计与实现

空间数据查询效率的优劣决定着三层体系结构空间信息系统的应用效果。空间数据缓存技术是提高空间数据查询效率的有效方法之一。在数据缓存技术逐渐成熟的今天,如何利用数据缓存技术来构建空间数据缓存是目前研究的热点。基于Microsoft新的数据缓存技术,借助常用的格网索引,在客户端和应用服务器端开辟空间数据缓存区,以降低数据的传输量,减少用户的请求响应时间,从而提高空间数据查询效率。在利用版本控制技术实现空间数据缓存的同时,解决客户端、应用服务器和数据库服务器的数据一致性问题,确保空间数据的有效性和可用性。     

航向测量仪在车载三维信息采集系统中的应用

航向测量仪(Vector Sensor)的作用就是为车载惯导系统初始化提供所必须的航向角;根据不同的应用环境和特性,用航向测量仪获取在各种条件下的航向角数据,通过对这些数据在精度、稳定性以及解算时间等性能上的对比分析,得到该仪器的最佳工作状态,进而获取该状态下的航向数据;通过实际的实验测试,此状态下的数据在精度和稳定性方面都能满足惯导系统的需求。     

基于影像的地图服务方法研究

随着当前用户对空间信息服务需求的不断提升,地图服务技术应用得到更多的关注,而对于G IS领域的地图数据,多为矢量格式,数据量非常大,故目前广泛采用的即时生成地图发布技术仍存在着服务器分析时间长、客户端浏览速度慢等问题,而基于预生成技术的影像地图服务方法则弥补了这些缺陷。主要分析基于网络的动态地图服务发布的研究现状,特别对基于切片方式的预生成影像地图服务的实现方法进行深入探讨,最后对两种主流W eb地图服务方法的性能进行对比分析。     

寻路地图设计中相关问题研究

以郑州市街头的寻路地图为例,对其设计现状进行了分析,指出了这些地图的特点和其在设计上存在的不足之处.根据寻路地图的目的和用户的特殊性,制作了寻路地图样图,并就其在定向和路线选择等方面进行了试验,对实验结果进行了分析和研究.结果表明地图内容、参考方向和地图符号是影响用户定向和路线选择的主要因素.以此为基础,得出了寻路地图设计中应当遵循的一些规律.