Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg²⁺, Fe²⁺, Mn²⁺, Co²⁺ and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L^-1 and 4.33 mg m L^-1 min^-1, respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.     

阿尔金造山带南缘晚奥陶世赞岐质闪长岩的发现及其地质意义

本文以阿尔金造山带南缘出露的高镁赞岐质闪长岩为研究对象,通过系统的野外地质工作、显微岩相学、年代学和全岩地球化学分析,探讨其成因类型和源区性质,限定了阿尔金造山带构造演化格架。闪长岩LA-ICP-MS锆石U-Pb测年结果表明其形成年龄为(445±3) Ma,属晚奥陶世。地球化学数据显示:闪长岩Al₂O₃含量为15.11%~16.19%,且具有高MgO(3.93%~5.29%)和K₂O(1.68%~2.67%)含量的特征,属于高镁钙碱性准铝质岩石;闪长岩具有较高的Cr(29.3×10^-6~140×10^-6)、Ni(27.1×10^-6~43.5×10^-6)、Ba(606×10^-6~936×10^-6)、Sr(513×10^-6~734×10^-6)和Y(26.4×10^-6~31.1×10^-6)含量,与典型的高镁赞岐岩类地球化学特征一致;稀土配分图和微量元素蛛网图显示闪长岩样品富集轻稀土元素和大离子亲石元素(如Rb和K),而亏损高场强元素(如Nb、Ti和Zr),表现出与俯冲带环境岩浆岩相似的性质;高Ti/Zr比值(48~60)与Mg^#(46.74~53.06)、高Cr(29.3×10^-6~140×10^-6)与高Ni(27.1×10^-6~43.5×10^-6)含量等暗示闪长岩岩浆源区来自遭受俯冲组分交代过的富集岩石圈地幔。岩石成因分析表明,阿尔金造山带南缘晚奥陶世高镁赞岐质闪长岩是后碰撞构造环境下俯冲板片断离诱发地幔楔发生减压熔融而形成,代表了早古生代最强烈的区域性幔源基性岩浆侵入事件。     

基于知识推理的土地退化程度评价模型

引入知识推理技术构建了一种新的土地退化程度评价模型(KR_LDAM)。模型集成了可变精度粗集和模糊神经网络两种智能化推理方法进行土地退化程度评价因子提取和退化程度分级,系统地消除了土地退化程度评价过程中专家经验知识主观不一致性以及客观数据不确定性的影响,解决了退化程度划分的模糊性和渐进性问题。选取黄土高原风沙侵蚀区域陕西省横山县为研究区域进行实例研究和模型验证。     

Petrogenesis of the Xihuashan Granite in Southern Jiangxi Province,South China: Constraints from Zircon U-Pb Geochronology,Geochemistry and Nd Isotopes

Mesozoic granitic intrusions are widely distributed in the Nanling region,South China.Yanshanian granites are closely connected with the formation of tungsten deposits.The Xihuashan granite is a typica...     

SENSITIVITY TESTS OF INTERACTION BETWEEN LAND SURFACE PHYSICAL PROCESS AND ATMOSPHERIC BOUNDARY LAYER*

In this paper,an interactive model between land surface physical process and atmosphere boundary layer is established,and is used to simulate the features of soil environmental physics,surface heat fluxes,evaporation from soil and evapotranspiration from vegetation and structures of atmosphere boundary layer over grassland underlying.The sensitivity experiments are engaged in primary physics parameters.The results show that this model can obtain reasonable simulation for diurnal variations of heat balance,soil volumetric water content,resistance of vegetation evaporation,flux of surface moisture,and profiles of turbulent exchange coefficient,turbulent momentum,potential temperature,and specific humidity.The model developed can be used to study the interaction between land surface processes and atmospheric boundary layer in city regions,and can also be used in the simulation of regional climate incorporating a mesoscale model.     

辽宁弓长岭矿区混合花岗岩地球化学特征及成因

经分析辽宁弓长岭二矿区混合花岗岩的主量元素、微量元素和稀土元素特征,对混合花岗岩的成因研究表明: 主量元素中Fe2O3、FeO、MgO 和CaO 含量较低,K2O 和Na2O 含量较高; 微量元素分配曲线属右倾型,强不相容元素富集; 轻稀土元素分馏明显,呈明显的Eu 负异常,但Ce 的正异常不明显,为典型壳型花岗岩稀土特征。弓长岭二矿区混合花岗岩原岩为富铝的沉积岩,混合花岗岩为部分熔融成因。     

美国极地钻探科学目标分析与钻探技术进展

极地钻探是获取极地冰层或冰下环境样品和在极地冰层或冰下布放科学观测仪器的最直接方法,是开展极地科学研究的必要技术手段。美国是开展极地钻探较早的国家之一,也是极地钻探强国。相比美国,我国极地钻探技术尚处于起步阶段。本文以《美国冰钻委员会长期科学规划2021-2031》为基础,结合其官方网站和相关文献资料,梳理了美国极地钻探科学目标和极地钻探技术现状,并简要介绍了过去10年美国极地钻探的现场工作情况及其在未来3年的工作计划,以期为我国极地钻探发展提供参考。     

星载极化SAR数据模拟

介绍了自行开发的星载极化SAR数据计算机模拟系统，此系统采用的模拟方法遵循了星载极化SAR系统实际运行的情况，按发射脉冲时间序列，分别依次计算各发射脉冲相应的回波数据，与常用的以点目标为基础逐点模拟方法相比，计算效率高，现贴近实际系统，便于模拟各种误差的影响和保持其对所有目标回波影响的一致性和相关性。给出了星载极化SAR数据模拟的实例。     

三颗河外射电源第一历元的VLBI图像

本文给出利用上海、乌鲁木齐、意大利ＮＯＴＯ和南非ＨａｒｔＲＡＯ射电望远镜,对三颗河外射电源（１５３２＋０１６,２３２００２０,２３５１１５４）,在５ＧＨｚ频率上的第一历元ＶＬＢＩ观测成图结果     

Assessing the vegetation canopy influences on wind flow using wind tunnel experiments with artificial plants

Wind erosion causes serious problems and considerable threat in most regions of the world. Vegetation on the ground has an important role in controlling wind erosion by covering soil surface and absorbing wind momentum. A set of wind tunnel experiments was performed to quantitatively examine the effect of canopy structure on wind movement. Artificial plastic vegetations with different porosity and canopy shape were introduced as the model canopy. Normalized roughness length (Z ₀/H) and shear velocity ratio (R) were analyzed as a function of roughness density (λ). Experiments showed that Z ₀/H increases and R decreases as λ reaches a maximum value, λ _max, while the values of Z ₀/H and R showed little change with λ value beyond as λ _max.