Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.     

Micromorphological-investigation on the Earth's Critical Zone

Soils, the Earth＇s Critical Zone, play a vital and fundamental role in the Earth＇s terrain eco- environment system and human life. Although the concepts of soil/quality and sustainable development have been introduced for decades, we are still failing to understand how well soils function and they need tobe cared and protected. We have been increasingly witnessing and becoming the victim of shrinking forests,     

潜艇指挥台围壳对阻力和伴流场影响数值研究

采用Reynolds平均Navier-Stokes(RANS)方法计算潜艇三维粘性流场,分析潜艇指挥台围壳对潜艇水动力性能的影响.采用全附体SUBOFF模型验证了CFD方法,通过将螺旋桨盘面处的实效伴流场、艇体表面压力分布以及模型总阻力的模拟结果与Taylor船池的实验结果进行对比.比较结果显示CFD计算结果与实验数据具有很好的一致性,表明CFD方法可以用于潜艇指挥台围壳设计的水动力计算.通过数值计算研究指挥台围壳的高度和在艇上的分布位置对其后方的流场、螺旋桨盘面处的伴流场和阻力的影响.     

雄性半滑舌鳎雄激素受体基因的克隆与表达分析

利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.     

2-[[(2-氨基苯基)亚胺]苯甲基]-4-氯苯酚晶体结构的量子化学研究

采用密度泛函理论(DFT)B3LYP方法,运用Gaussian03量子化学程序包,在B3LYP/6-31G*基组水平上对合成的化合物2-[[(2-氨基苯基)亚胺]苯甲基]-4-氯苯酚的晶体结构进行优化,计算了分子稳定构型的总能量、前线分子轨道能量、原子自然电荷布局、自然键轨道(NBO)及稳定化能等.计算结果与通过单晶X-射线衍射法测得的晶体结构的实验结果吻合.此项研究可为异双希夫碱配体及其金属配合物的合成及实际应用提供理论参考.     

NUFFT-Based Iterative Reconstruction Algorithm for Synthetic Aperture Imaging Radiometers

Synthetic aperture imaging radiometers (SAIRs) are powerful sensors for high-resolution observation of the Earth at low microwave frequencies. However, the future application of this new technology is limited by its large number of element antennas and receiving channels. In order to further reduce the complexity of the whole system, the circular and rotating array configurations have been proposed. Their disadvantages lie primarily in more complicated image reconstruction, because the conventional fast Fourier transform (FFT) method could not be used directly for image reconstruction. This letter introduces an iterative method for the reconstruction of radiometric brightness temperature maps from nonuniform visibility function samples. This method combines the conjugate gradient algorithm with min-max nonuniform FFT approach, which has been shown to provide considerably improved image quality relative to the traditional gridding method. Simulations for SAIRs were performed and showed better image quality in comparison to the traditional frequency interpolation method.     

Pristine or altered: low-Mg calcite shells survived from massive dolomitization? A case study from Miocene carbonates

Low-Mg calcite shells have been widely used to reconstruct the chemistry of ancient seawater. There is always a question: are the shells chemically pristine? This paper presents the isotope and elemental geochemistry of low-Mg calcite bivalve shells in late Miocene platform carbonates, SE Spain. The platform carbonates were extensively dolomitized, and limestone is restricted to older stratal units, and to units mainly in topographically higher and more landward strata. Low-Mg calcite oyster shells were completely dissolved out in the basinward dolomite, but are well preserved in the limestone. These shells appear to retain the original growth microstructures, based on hand samples. Under the microscope, however, dissolution and recrystallization, as well as pristine growth lines are all present. Sr isotopes in these shells range from that of normal Miocene seawater to radiogenic values. δ¹⁸O and δ¹³C values, and Mg, Sr, and Na concentrations in these shells are rather variable. The high end members are consistent with the typical values of modern/late Miocene normal-seawater low-Mg calcite shells, whereas the low end members are close to those of diagenetic calcite cements, which have low δ¹⁸O, δ¹³C, Sr and Na values, and radiogenic Sr. The Nijar shells were altered physically and chemically to different degrees by diagenesis, although these shells are consistent with some “criteria” of unalteration. The isotopic and trace-element data collected in altered and pristine (or less altered) portions coexisting in the same shells are clearly differentiable. Quantitative simulation of covariations of geochemical pairs indicates that solid mixing of unaltered and altered portions by sampling is consistent with the variations in isotopic and elemental data recorded in the Nijar shells. The geological significance of this study is that ancient fabric-retentive calcite shells may have been altered geochemically although they may appear pristine. Calcite shells that underwent intensive diagenesis should be examined rigorously under the microscope, coupled with investigations of multiple geochemical proxies to assess chemical alteration. Only the data of unaltered shells can be used to reconstruct the chemistry of ancient seawater.     

塔里木盆地石炭系层序地层研究

应用地震、钻井、测井、岩心、露头及分析化验资料,将塔里木盆地石炭系划分为4个地震层序和6个沉积层序,其中Csq1、Csq2、Csq5层序为工型层序,Csq3、Csq4、Csq6层序为Ⅱ型层序,与标准的工型层序比较,盆地内Ⅰ型层序低位体系域以河流沉积体系和低位扇三角洲沉积体系为特征,缺失盆底扇、斜坡进积复合体等深水沉积体系;Ⅱ型层序中陆架边缘体系域不发育,主要的沉积体系以浅水碳酸岩台地和海岸平原为特征。石炭纪早期层序形成在起伏不平的剥蚀面之上,以填平补齐的充填沉积为主,中后期层序则发育在宽阔平坦的盆地内,层序地层学格架模式可用Van的被动大陆边缘模式来描述。     

The study on a new technology of waterway information system

It is a lot limitations to survey and design waterway by the CAD technique,due to timely variation of hydrology and ecology in the complicated estuary area,such as Changjing River estuary.In this paper,the advantages of GIS technique is applied to manage and process the multi kind,multi scale and multi time hydrographic parameters,including water depth,current velocity,tidal,waves,wind speed,temperature and salinity etc.,also to deal with different projection,different scale charts.Meanwhile,in the paper,combination techniques of GIS and CAD has been developed for unification of the charts for efficient management,two-way automatic carts conversion between GIS and CAD,automatic cutting and assemble of a number of Charts,flight path of the projection of spatial data and coordinate transformation.This new technique has been applied to survey and design waterway of Changing River estuary.The result shows that the combination technology of GIS and CAD has application potentiality in the waterway survey and design.     

The black water around the Changjiang (Yangtze) Estuary in the spring of 2003

The Changjiang (Yangtze) Estuary is located in the East China Sea shelf with shallow water. Affected by the tide mixing and the runoff of the Changjiang River and the Qiantang River, the turbidity is very high. Generally, the water-leaving radiance is high in the turbid water because of the large particle scattering. Based on the in-situ data and ocean color remote sensing data of SeaWiFS, it was found that there was a black water region with the normalized water-leaving radiances less than 0.5 mW/(cm2·μm2·...