日本鳗鲡早期阶段耳石日生长轮形成的周期

于1990年4月从江苏太湖搜集日本鳗鲡亲鱼暂养在天津滨海虾场8t玻璃钢水槽中，经人工催熟催产后孵出仔鳗，对孵出仔鳗连续取样；于1989年4月在长江口采集白仔鳗，观察和比较二者的耳石生长轮形成。结果表明，（1）人工繁殖仔鳗耳石第一个生长轮是在孵出后第一天形成的，轮纹形成具有24h周期性；仔鳗孵出后生长天数与生长轮数关系的回归方程以y=0．23＋0．91x表示，其中，x为孵出后的天数，y为生长轮数;在人工繁殖仔鳗耳石上没有观察到“孵化标记”轮。（2）白仔鳗耳石中心核与“孵化标记”轮之间存在日生长轮。（3）人工繁殖仔鳗与白仔鳗耳石中心核同第一个生长轮比较表明，前者小于后者。     

Three-dimensional numerical experiments on tidal exchange through a narrow strait in a homogeneous and a stratified sea

We have investigated the three-dimensional Lagrangian motion of water particles related with tidal exchange between two basins with a constant depth connected through a narrow strait and the effects of density stratification on the exchange processes by tracking a number of the labeled particles. Tide-induced transient eddies (TITEs), which are similar to those in two-dimensional basin, are generated behind the headlands. Upwelling appears around the center of the eddy and sinking around the boundary. When the basins are filled with homogeneous water, a pair of vortices are produced in the vertical cross section of the strait due to bottom stress, with upwellings along the side walls of the strait and sinking in the center of the strait. These circulations form the horizontally convergent field in the cross-strait direction in the upper layers while the horizontal divergence takes place in the bottom layer. These vertical water-motions produce the three-dimensional distribution of velocity shear and phase lag of the tidal current around the strait, and the Lagrangian drifts of water particles become large. As a result, water exchange through the strait is greatly enhanced: The water exchange rate reaches 94.1% which is much larger than that obtained in the vertically integrated two-dimensional model. When the basins are stratified, the stable stratification suppresses the vertical motion so that a pair of vertical vortices are confined in the lower layers. This leads to a decrease in the exchange rate, down to 88.6%. Our numerical results show that the three-dimensional structure of tidal currents should be taken into account in tidal exchange through a narrow strait.     

A new type of Shipboard Meteorological Satellite Receiving-processing System

INTRODUCTIONTheSMSRPSisstalledontheshipnavigatingovertheseaandreceivessatellitecloudmapsatanytimetoprovidereliablereal-timedataofmeteorologyandocean.Itisanimportantequipmentforsafeguardofshipnavigation.Chinahasthousandsofoceanicships.Butalmostallships,withonlyveryfewexception,arenotstalledbySMSRPS.Thecausesareasfollows:1.Thetechniqueisverycomplicated.2.Thedevelopmentcostisveryhigh.3.Ifweintroduceforeignequipment,thecostistooexpensive.4.Foreignequipmentanditssoftwarearecompletelyclo…     

南大洋深海嗜冷菌2-5-10-1及其低温脂肪酶的研究

从南大洋普里兹湾的水样中筛选到一株产低温脂肪酶的深海嗜冷菌2-5-10-1,对其生长及产酶情况和酶性质做了初步研究.该菌株的最适生长温度为5℃,此时分泌的胞外脂肪酶最多;添加Tween80,橄榄油可显著促进脂肪酶的产生.该脂肪酶的最适作用温度为35℃,在0～20℃均保持较高的酶活性,在0℃可保持37%的相对酶活性;酶的最适作用的pH值为7.5,在pH6～9的范围内均存在较高酶活性;对热较敏感,在60℃保温15min可丧失50%以上的酶活性.该脂肪酶的催化作用不需要金属离子的参与,Cu2+和Zn2+对酶活有着强烈的抑制作用.     

Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.     

大生活用海水生化处理技术研究进展

采用海水作为大生活用水最结解沿海城市淡水资源紧缺局面的有效途径之一。该技术得以推广应用的技术关键是大生活用海水的后处理技术，文章综述了大生活用海水的生化处理技术的研究现状，并提出了今后的研究方向。     

Studies on Genus Cryptomonas from China seas

ＩＮＴＲＯＤＵＣＴＩＯＮＣｒｙｐｔｏｍｏｎａｓｉｓｕｎｉｃｅｌｌｕｌａｒｂｉｆｌａｇｅｌｌａｔｅｗｈｉｃｈｉｓａｃｏｍｍｏｎｇｅｎｕｓｏｆＣｒｙｐｔｏｐｈｙｔａ .Ｔｈｅｃｅｌｌｓａｒｅｕｓｕａｌｌｙｓｔｒｏｎｇｌｙｃｏｍｐｒｅｓｓｅｄｌａｔｅｒａｌｌｙ .ＴｈｅｒｅａｒｅｏｎｅｏｒｔｗｏｌａｒｇｅＣｈｌｏｒｏｐｌａｓｔｓｔｈａｔｃｏｎｔａｉｎｃｈｌｏｒｏｐｈｙｌｌａ ,ｃ ,ｓｏｍｅｃｈａｒａｃｔｅｒｉｓｔｉｃｃａｒｏｔｅｎｏｉｄｓａｎｄｐｈｙｃｏｂｉｌｉｎｐｉｇｍｅｎｔｓ (Ｈｕｅｔａｌ.,1 997;Ｗｅｉ…     

碳水化合物的组合合成

碳水化合物的组合合成是一项新兴技术，该技术可以在短时间内合成大量用于进行生物活性筛选的寡糖及拟糖物。液相与固相合成技术可以极大地加快药物研究与开发进程。作者针对该技术在碳水化合物合成方面的研究进展情况进行讨论。     

沉积物对磷酸盐的吸附与释放

本实验研究黄河口水文站沉积物对黄河水磷的吸附释放过程和东海１１１号站位沉积物对海水磷的吸附释放过程。并摸拟黄河沉积物在海水中磷的吸附释放过程。结果表明：底沉积物对磷的吸附相当迅速，无论是河泥还是海泥在２４ｈ内均能达到吸附平衡。吸附量与沉积物的量和水中原有磷的浓度有关，在不同的ｐＨ条件下，沉积物对磷的吸附是有显著差别的     

盐渍洼地蟹种培育试验

盐渍洼地种蟹培育是解决成蟹养殖种苗来源的重要途径，文中介绍的幼蟹放养，饲养管理与水质控制，幼蟹生长率与土增重量的变化，蟹种成活率与回捕率等对蟹种池墉强化培育提供了经验与理论依据。