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George Galdorisi 《Ocean & Coastal Management》1994,25(3)
The 1982 United Nations Convention on the Law of the Sea prescribed extensive rules of behavior for the international community in the oceanic regime. Some of the most important provisions of the Convention are those regarding the freedom of navigation and overflight in various maritime zones, i.e. territorial seas, international straits, archipelagos, exclusive economic zones and the high seas.While most coastal nations are complying with the navigation and overflight provisos of the Convention, some are not. Where excessive maritime claims exist, the US has evolved an ambitious Freedom of Navigation (FON) Program which challenges these excessive claims. This Program was initiated under the Carter Administration in 1979 and has continued, unabated, ever since.As the international community prepares for the day when the 1982 Convention becomes universally-recognized international law, there are likely to be periodic excessive maritime claims. In cases where the Convention's dispute settlement provisos fail to resolve excessive claims, the community of nations may find it useful to use the US FON Program as an example for the enforcement of maritime rules. 相似文献
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Studies of xenobiotic metabolism and the regulation of enzyme systems for their metabolism (Phase I and II enzyme systems) require large numbers of animals, intensive use of experimental aquarium systems and in some instances can pose major problems when the compounds are scarce, expensive or too toxic to be disposed of easily. Previous studies have demonstrated the usefulness of isolated primary hepatocytes of fish for metabolic and enzyme induction studies. Functionally and structurally competent hepatocytes were isolated from juvenile plaice and after overnight acclimation to culture conditions, were exposed to a variety of PAHs for 24h. The levels of CYP1A1, phenol UDPGT and GST-A mRNAs were then estimated by slot blotting and hybridisation to their cDNA probes. The method enabled rapid and easy determination of the structure/activity relationships of these compounds as inducers of these key Phase I and II enzymes, and is potentially useful for screening large numbers of compounds both as an invitro toxicity test and for mechanistic studies. 相似文献
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Baseline data for hepatic xenobiotic metabolizing biomarker enzyme activities were obtained for artificially reared tilapia Oreochromis niloticus, and were compared with those of the plaice (Pleuronectes platessa) and rainbow trout (Onchorynchus mykiss). Basal activities exhibited species variations with notably higher CYP1A and phenol UGT activities and lower GST activity in plaice than the freshwater species. Interspecies relationships between gene families determined by immunoblotting and substrate-activity profiles demonstrated the presence of homologous CYP1A and CYP3A enzymes in all three species, alpha class GSTs in plaice and trout, mu and pi class GSTs in trout and theta class GSTs in plaice and tilapia. CYP1A of tilapia was induced by 3-MC or PBO treatment, whilst CYP3A was induced by PCN treatment. 相似文献