Structural Studies of a UDP-glucuronosyltransferase gene from the plaice (Pleuronectes platessa)

A partial length cDNA coding for the putative PAH-inducible phenol-conjugating UDP-glucururonosyltransferases (UGT) isoform of plaice was used to isolate overlapping clones from a plaice genomic library. Sequence analysis revealed the presence of a complete gene spanning 4.1 kb for a plaice UGT which showed a strong conservation in exon structure, amino acid character and amino acid sequence with mammalian UGT1 family genes, although additional alternative upstream exon 1s were not identified in the present study. Southern blot analysis revealed a low copy number for the gene and some degree of structural polymorphism in gene structure between individuals. This was also reflected in the finding that there were significant variations between the nucleotide sequences of the plaice gene and the cDNA previously isolated from a different individual fish. Future studies will investigate the possibility that there may be phenotypic variations which could lead to alterations in susceptibility to pollutant toxicity.     

A piscine glutathione S-transferase which efficiently conjugates the end-products of lipid peroxidation

A cDNA clone for glutathione S-transferaseA (GSTA) from plaice (Pleuronectes platessa) was expressed in Eschericia coli (E. coli) and purified to homogeneity by S-hexylglutathione affinity chromatography. When compared to literature values for a variety of purified mammalian GSTs, the heterologously expressed purified plaice enzyme had moderate activity towards the model substrate 1,2-chloro-2,4-dinitrobenzene (CDNB) and exhibited a Km of 2.5 ± 2 mM and Vmax of 30.9 ± 2.3 μmol min⁻¹ mg⁻¹. It had little or no activity towards several other model GST substrates including 1,2-dinitrochloro-4-benzene (DCNB), ethacrynic acid (EA), and p-nitrobenzylchloride (NBC). However plaice GSTA was a relatively efficient catalyst for the conjugation of a series of alk-2-enals and alk-2,4-dienals and also 4-hydroxynonenal. The highest activity observed with this series of substrates was with trans-non-2-enal with a Km of 17.9 ± 2.2 μM and a Vmax of 3.01 ± 0.57 μmol min⁻¹ mg⁻¹. These unsaturated alkenals have been identified in cells and cell extracts as highly toxic products arising from peroxidation of unsaturated fatty acids particularly during periods of oxidative stress. Fish are relatively rich in polyunsaturated fatty acids and thus GSTA mediated conjugation may be an important mechanism for detoxifying peroxidised lipid breakdown products.     

Crystallization of the Little Three layered pegmatite-aplite dike, Ramona District, California

Subhorizontally layered pegmatite-aplite bodies are characterized by fine-grained, sodic to granitic aplite that is usually juxtaposed abruptly above by much coarser-grained, commonly graphic potassic pegmatite. Although well studied, there currently is little concensus as to how such dikes form. The Little Three dike near Ramona, California, is representative of such zoned bodies in this and other regions, and contains discontinuous miarolitic pockets near the base of the graphic pegmatite zone. Tourmaline, garnet, biotite, and muscovite show no changes in major- or minor-element compositions indicative of progressive magmatic fractionation until the immediate vicinity of the main miarolitic zone, where they record abrupt and extreme enrichments in Li, F, and Mn. There is no correlation of chemical changes in the dike with the appearance of small miarolitic vugs well below the main miarolitic zone, nor is there any indication that the aplite, graphic pegmatite, or miarolitic pockets represent separate magma injections. The chemistries of tourmaline, garnet, and micas, however, preclude conventional models of Rayleigh fractionation or traditional zone refining. Textural features and modeled cooling histories indicate that the dike cooled quickly and might have solidified partially or totally to glass before crystallization commenced. Geothermometry based on the compositions of coexisting plagioclase and homogeneous, nonperthitic K-feldspar indicates inward crystallization of the dike, from ∼400–435 °C at the margins to ∼350–390 °C within 20–30 cm of the pocket horizon, then a sharp decrease to 240–275 °C in the pockets where K-feldspar is perthitic. We interpret the feldspar geothermometry (except perhaps in the miarolitic cavities) to reflect the temperatures at crystallization fronts that advanced into the pegmatite, first from the foot wall and eventually joined by a similar front downward from the hanging wall. Crystallization down from the hanging wall may have commenced after ∼70–80% of the foot wall aplite had crystallized. The very abrupt increases of Li, Mn, and F in tourmaline and garnet near the miarolitic zone appear to be explained best by the process of constitutional zone refining, in which a fluxed crystallization front sweeps an incompatible element-enriched boundary layer through a solid or semi-solid. After these two highly fluxed boundary layers merged near the main miarolitic zone, compositional evolution could have proceeded by crystal-melt fractionation. Received: 24 March 1998 / Accepted: 10 March 1999     

Glacial Geomorphologic Maps of Llanquihue Drift in the Area of the Southern Lake District, Chile

We present four glacial geomorphic maps of Llanquihue drift in the region of the southern Lake District, Seno Reloncaví, and the eastern half of northern Isla Grande de Chiloé, Chile. These maps form the basis of an interdisciplinary research project to establish a paleoclimate reconstruction of the last glacial cycle in the middle latitudes of the Southern Hemisphere. Therefore we stressed morphologic rather than lithologic units in our mapping program in order to delineate the glacial geomorphic forms that are surface manifestations of former ice-marginal fluctuations. The resulting maps show the lobate shape and low surface slopes of the former piedmont glaciers that, at the last glacial maximum, spread westward from the Andes into the Valle Centralto fill Lago Puyehue, Lago Rupanco, Lago Llanquihue, Seno Reloncaví, Golfo de Ancud, and northern Gulfo Corcovado. These maps are the framework to tie together in this volume the stratigraphic sections and radiocarbon samples from within the Llanquihue moraine belts as well as the pollen cores from surface mires on the belts.     

Moraine Exposure Dates Imply Synchronous Younger Dryas Glacier Advances in the European Alps and in the Southern Alps of New Zealand

Samples taken from the top surfaces of boulders on the Lake Misery moraines at Arthur's Pass, in the Southern Alps of New Zealand, were analysed for ¹⁰Be by accelerator mass spectrometry. Exposure ages calculated with the currently accepted production rate, along with scaling corrections for sample latitude and elevation (42°50'S, 960 m), are: 9300 ± 990, 11,000 ± 1360, 11,410 ± 1030, 12,050 ± 960, and 12,410 ± 1180 years. We consider the date of 9300 years to be an outlier, not included in our mean exposure age of 11,720 ± 320 years for the Lake Misery moraines. Based on exposure ages and geomorphologic similarities, we compare the Lake Misery moraines with an Egesen moraine complex at Julier Pass in the Swiss Alps (46°30'N, 2200 m). Based on the ¹⁰Be, ²⁶Al, and ³⁶Cl exposure ages of three boulders, we calculate a mean exposure age of 11,750 ± 140 years for the outer Egesen moraine at Julier Pass. Based solely on ¹⁰Be measurements, we obtain a mean exposure age of 11,860 ± 210 years for this outer moraine. Egesen moraines in the Swiss Alps represent glacier readvance during the Younger Dryas cold reversal, based on regional correlations and on basal radiocarbon dates from bogs located up-valley of Egesen moraines. The exposure dates from Arthur's Pass and Julier Pass show synchronous glacier advances both in the Southern Alps and in the European Alps during the European Younger Dryas chronozone of Mangerud et al .     

Glacially Folded Outwash Near Lago Llanquihue, Southern Lake District, Chile

Folded outwash occurs in four distinct clusters in an arcuate arrangement just west of the terminal Llanquihue moraines deposited by the Lago Llanquihue piedmont ice lobe at the last glacial maximum. These clusters are physically connected along the eastern side to the Llanquihue terminal moraines, and along the western side to the Llanquihue outwash plain. Each cluster consists of three to eleven elongated ridges. The maximum height of individual ridges varies from cluster to cluster beween 18 and 28 m; the maximum length of individual ridges is between 93 and 1074 m. The average orientation of the ridges ranges over a 60° sector relative to former ice-flow direction. The folded out-wash sediments are cut by two distinct internal fault systems with only a faint surface expression below the Holocene top soil.
The folded outwash ridges are interpreted as a push moraine system produced by the same mechanical forces that act in a critically tapered wedge. The folded sediment is a sandy gravel with an angle of friction on the order of φ = 40°. Interpretations of structural data and of mechanical comparisons point to a basal thrust plane in a sand unit with φ between 24° and 30° and with a pore water pressure index of l = 0.7.
It is very unlikely that the observed and analyzed features wereformed under permafrost conditions.     

Teleseismic shear wave splitting measurements in noisyenvironments

High noise levels hamper teleseismic shear wave splitting measurements, which bandpass filtering does not always help. To investigate how robust splitting measurements are to noise, we analysed a set of synthetic records with known splitting parameters and added fixed levels of noise. In the presence of weak anisotropy, single-waveform splitting measurements are unreliable when operating with noisy data sets. A practical rule in terms of S/N ratio and splitting delay time parameters is that splitting is confidently detectable at S/N > 8, regardless of the wave's original polarization orientation. However, for the evidence of weak anisotropy to be detectable and measurable at an S/N value of 4, the backazimuth separation of the phases from the fast polarization direction needs to be higher than 20°. Stacks of individual measurements consistently yield reliable results down to S/N values of 4. Applying stacking to data from DSB (Dublin, Ireland), the fast polarization direction φ and lag time δt are 58° and 0.95  s. This orientation reflects surface trends of deformation in the area, as found elsewhere in the UK. Our result thus reinforces the proposed model that the detected anisotropy in the British Isles originates from lithospheric coherent deformation preserved from the last main tectonic episode.     

Intersecting views of gully erosion in South Africa

Gully erosion is an environmental problem recognized as one of the worst land degradation processes worldwide. Insight into regional gully perturbations is required to combat the serious on- and off-site impacts of gullying on a catchment management scale. In response, we intersect different perspectives on gully erosion-specific views in South Africa (SA), a country that exhibits various physiographic properties and spans 1.22 million km². While the debate surrounding gully origin continues, there is consensus that anthropogenic activities are a major contemporary driver. The anthropogenic impact caused gullying to transcend climatic, geomorphic, and land-use boundaries, although it becomes more prominent in central to eastern SA. Soil erodibility plays a crucial role in what extent of gully erosion severity is attained from human impact, contributing to the east–west imbalance of erosion in SA. Soil erosion rates from gullying and badlands are limited but suggest that it ranges between 30 and 123 t ha⁻¹ yr⁻¹ in the more prominent areas. These soil loss rates are comparable to global rates where gullying is concerned; moreover, they are up to four orders of magnitude higher than the estimated baseline erosion rate. On a national scale, the complexity of gullying is evident from the different temporal timings of (re)activation or stabilizing and different evolution rates. Continued efforts are required to understand the intricate interplay of human activities, climate, and preconditions determining soil erodibility. In SA, more medium- to long-term studies are required to understand better how changing control factors affect gully evolution. More research is needed to implement and appraise mitigation measures, especially using indigenous knowledge. Establishing (semi)-automated mapping procedures would aid in gully monitoring and assessing the effectiveness of implemented mitigation measures. More urgently, the expected changes in climate and land-use necessitate further research on how environmental change affects short-term gully erosion dynamics.