In vitro alternatives to whole animal testing. Comparative cytotoxicity studies of divalent metals in established cell lines derived from tropical and temperate water fish species in a neutral red assay

A simple vital dye (neutral red) uptake method for investigation of the acute toxicity of seven divalent metal cations to cell lines derived from the turbot (TF) and bluegill (BF-2) was evaluated. The method was extremely reproducible between replicate experiments on a week to week basis as well as being reproducible between different laboratories. Whilst there were some species-dependent differences in the relative toxicities of the metals there were good correlations between toxicity and the chemical softness (Σp) of the metal ions and also between in vivo and in vitro toxicities. These studies demonstrate that this simple and relatively inexpensive technique carried out in 96-well microplates may be extremely useful for evaluation of structure-toxicity relationships between related groups of compounds.     

Twelve years of tracking 52-Hz whale calls from a unique source in the North Pacific

A unique whale call with 50–52 Hz emphasis from a single source has been tracked over 12 years in the central and eastern North Pacific. These calls, referred to as 52-Hz calls, were monitored and analyzed from acoustic data recorded by hydrophones of the US Navy Sound Surveillance System (SOSUS) and other arrays. The calls were noticed first in 1989, and have been detected and tracked since 1992. No other calls with similar characteristics have been identified in the acoustic data from any hydrophone system in the North Pacific basin. Only one series of these 52-Hz calls has been recorded at a time, with no call overlap, suggesting that a single whale produced the calls. The calls were recorded from August to February with most in December and January. The species producing these calls is unknown. The tracks of the 52-Hz whale were different each year, and varied in length from 708 to 11,062 km with travel speeds ranging from 0.7 to 3.8 km/h. Tracks included (A) meandering over short ranges, (B) predominantly west-to-east movement, and (C) mostly north-to-south travel. These tracks consistently appeared to be unrelated to the presence or movement of other whale species (blue, fin and humpback) monitored year-round with the same hydrophones.     

Collocation resolution of the oceanic geoid based on satellite altimetry

The approach presented is directed toward a specific adaptation of the least‐squares collocation with noise, yielding smooth predictions of geophysical quantities. The smoothing corresponds here to a truncated gravity field equivalent to an (n’, n') spherical‐harmonic expansion. This is reflected in the truncation, at the degree n‘, of the pertinent covariance and cross‐covariance functions in most (but not all) instances. The smooth predictions of geophysical quantities, made in an equilateral grid corresponding to the truncation degree n‘, serve in constructing contour maps after having been densified for the needs of a contour routine. Such a densification is carried out efficiently via errorless collocation with the degree truncation n‘ throughout. Consistent with this procedure, “residuals” at observation points (i.e., discrepancies between the contour map and the data) are computed using the same algorithm. The complete collocation approach is utilized for a 2° resolution of the earth's gravity field with emphasis on the oceanic geoid, based on the residuals from a global spherical‐harmonic adjustment of SEASAT altimetry. The presented results include contour maps of geoid undulations and gravity anomalies. They are compared to the results of a point‐mass adjustment, another technique based on the spherical‐harmonic adjustment. The agreement between these two techniques is found to be excellent.     

Initial results and progress of the Mississippi delta sediment pore water pressure experiment

Abstract

This report describes the instrumentation, initial results, and progress of an experiment designed to measure and monitor submarine sediment pore water and hydrostatic pressures in a selected area of the Mississippi Delta. The experiment also is intended to monitor significant pressure perturbations during active storm periods. Initial analysis of the data revealed excess pore water pressures in the silty clay sediment at selected depths below the mudline. Continuous monitoring of the pore water and hydrostatic pressures was expected to reveal important information regarding sediment pore water pressure variations as a function of the geological processes active in the Mississippi Delta.     

Performance of pile-restrained flexible floating breakwaters

The overall performance of pile-restrained flexible floating breakwaters is investigated under the action of linear monochromatic incident waves in the frequency domain. The aforementioned floating breakwaters undergo only vertical structural deflections along their length and are held in place by means of vertical piles. The total number of degrees of freedom equals the six conventional body modes, when the breakwater moves as a rigid body, plus the extra bending modes. These bending modes are introduced to represent the structural deflections of the floating breakwater and are described by the Bernoulli–Euler flexible beam equation. The number of bending modes introduced is determined through an appropriate iterative procedure. The hydrostatic coefficients corresponding to the bending modes are also derived. The numerical analysis of the flexible floating breakwaters is based on a three-dimensional hydrodynamic formulation of the floating body. A parametric study is carried out for a wide range of structural stiffness parameters and wave headings, to investigate their effect on the performance of flexible floating breakwaters. Moreover, this performance is compared with that of the corresponding pile-restrained rigid floating breakwater. Results indicated that the degree of structural stiffness and the wave heading strongly affect the performance of flexible floating breakwaters. The existence of an “optimum” value of structural stiffness is demonstrated for the entire wave frequency range.     

Guest editorial

The Jason-1 satellite was launched on 7 December 2001 with the primary objective of continuing the high accuracy time series of altimeter measurements that began with the TOPEX/Poseidon mission in 1992. To achieve this goal, it is necessary to validate the performance of the Jason-1 measurement system, and to verify that its error budget is at least at the same level as that of the TOPEX/Poseidon mission. The article reviews the main components of the Jason-1 altimetric error budget from instrument characterization to the geophysical use of the data. Using the Interim Geophysical Data Records (16DR) that were distributed to the Jason-1 Science Working Team during the verification phase of the mission, it is shown that the Jason-1 mission is performing well enough to continue studies of the large-scale features of the ocean, and especially to continue time series of mean sea-level variations with an accuracy comparable to TOPEX/Poseidon.     

Private rights,public benefits: Industry-driven seabed protection

New Zealand has a large exclusive economic zone (EEZ) that contains a variety of marine habitats and commercially-important species. The commercial fishing industry operating within New Zealand's EEZ is of significant value to the economy and fisheries resources are managed through the extensive use of Individual Transferable Quotas (ITQs). One of the benefits of ITQs has been to better align some of the private incentives of quota owners with the public interest. These incentives contributed to an initiative proposed by the fishing industry to close large areas of New Zealand's EEZ to protect the seabed from trawling. These closed areas are termed benthic protection areas (BPAs) and protect the benthic biodiversity of about 1.1 million square kilometres of seabed—approximately 30% of New Zealand's EEZ. A significant proportion of New Zealand's known seamounts and active hydrothermal vents are protected by these closed areas. We describe and discuss the criteria used to select BPAs and some of the criticism of this marine protection initiative. We argue that the assignment of strong property rights in fishing resources was an important precondition to an industry initiative that has a significant public benefit. Where private and public interests are well aligned, government can adopt an enabling and facilitation role, ceding direct control of processes in order to get the results the align with the public interest.     

Assessment of relative slope stability of Kodiak shelf,Alaska, using high‐resolution acoustic profiling data

Abstract

The use of marine high‐resolution geophysical profiling data, seafloor soil samples, and accepted land‐based methods of analysis have provided a means of assessing the regional geotechnical conditions and relative slope stability of the portion of the Gulf of Alaska Continental Margin known as the Kodiak Shelf. Eight distinct types of soils were recognized in the study; the seafloor distribution of these indicates a complex geotechnical setting. Each soil unit was interpreted as having a distinct suite of geotechnical properties and potential foundation engineering problems. Seven categories of relative slope stability were defined and mapped. These categories range from “highest stability”; to “lowest stability,”; and are based on the degree of slope of the seafloor, type of soil underlying the slope, and evidence of mass movement. The results of the analysis indicate that the highest potential for soil failure exists on (1) the slopes forming boundaries between the submarine banks and the broad sea valleys, and (2) the upper portion of the continental slope, where evidence of past slope failure is common. Also of concern are gently sloping areas near the edges of submarine banks where evidence of possible tension cracks and slow downhill creep was found.     

Winkler's method overestimates dissolved oxygen in seawater: Iodate interference and its oceanographic implications

Dissolved oxygen in seawater has been determined by using the Winkler's reaction scheme for decades. An interference in this reaction scheme that has been heretofore overlooked is the presence of naturally occurring iodate in seawater. Each mole of iodate can result in an apparent presence of 1.5 mol of dissolved oxygen. At the concentrations of iodate in the surface and deep open ocean, it can lead to an overestimation of 0.52 ± 0.15 and 0.63 ± 0.05 μmol kg^− 1 of oxygen in these waters respectively. In coastal and inshore waters, the effect is less predictable as the concentration of iodate is more variable. The solubility of oxygen in seawater was likely overestimated in data sources that were based on the Winkler's reaction scheme for the determination of oxygen. The solubility equation of García and Gordon [Garcia H.E., Gordon, L.I., 1992. Oxygen solubility in seawater: Better fitting equations. Limnol. Oceanogr. 37, 1307–1312] derived from the results of Benson and Krause [Benson, F.B., Krause, D. Jr., 1984. The concentration and isotopic fractionation of oxygen dissolved in freshwater and seawater in equilibrium with the atmosphere. Limnol. Oceanogr. 29, 620–632] is free from this source of error and is recommended for general use. By neglecting the presence of iodate, the average global super-saturation of oxygen in the surface oceans and the corresponding efflux of oxygen to the atmosphere both have been overestimated by about 8%. Regionally, in areas where the degree of super-saturation or under-saturation of oxygen in the surface water is small, such as in the tropical oceans, the net air–sea exchange flux can be grossly under- or overestimated. Even the estimated direction of the exchange can be reversed. Furthermore, the presence of iodate can lead to an overestimation of the saturation anomaly of oxygen in the upper ocean attributed to biological production by 0.23 ± 0.07%. AOU may have been underestimated by 0.52 ± 0.15 and 0.63 ± 0.05 μmol kg^− 1 in the surface mixed layer and deep water, while preformed phosphate and preformed nitrate may have been overestimated by 0.004 ± 0.001 and 0.06 ± 0.02 μmol kg^− 1 in the surface mixed layer, and 0.005 ± 0.0004 and 0.073 ± 0.006 μmol kg^− 1 in the deep water. These are small but not negligible corrections, especially in areas where the values of these parameters are small. At the increasing level of sophistication in the interpretation of oxygen data, this source of error should now be taken into account. Nevertheless, in order to avoid confusion, an internationally accepted standard needs to be adopted before these corrections can be applied.