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81.
摘要:目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。 相似文献
82.
China Ocean Engineering - A consensus algorithm proposed in the paper is applied to tackle remarkable problems of unmeasurable velocities, the environmental disturbances, and the limited... 相似文献
83.
利用UMRAO数据库22个耀变体源的偏振数据,根据Lazarian&Pogosyan理论模型分析研究了耀变体射电波段的偏振随波长的变化,得出不同类的耀变体偏振随波长变化的原因。结果表明,与Lazarian&Pogosyan理论模型吻合较好。由此得出如下结论:(1)反常去偏振耀变体源中反常法拉第旋光起主导作用,反常去偏振相对较少;(2)热辐射与同步辐射混合作用,高频波段的偏振度小于低频波段的偏振度(反常去偏振);(3)当被观测波段波长小于吸积盘热辐射对应的维恩波长时,高频波段的偏振度大于低频波段的偏振度(常规去偏振)。 相似文献
84.
现有铬盐生产技术阻碍了铬盐行业的清洁化发展,研发新型铬盐生产技术实属必要。中国科学院青海盐湖研究所自行研发出一套以电化学法为基础的铬盐清洁生产工艺,涵盖三条大工艺和四种母系铬盐产品。包括氢氧化钠溶液中电化学氧化铬铁制备铬酸钠联产三氧化二铬和三氧化二铁工艺,离子膜电解法从铬酸钠合成重铬酸钠工艺,以及离子膜电解法从重铬酸钠合成铬酸酐工艺。相比现有铬盐生产技术,该技术的优势主要体现在以下五点:温和化、去渣化、高值化、自动化和高效化。目前相关研发工作业已走出实验室,走过产业化中间试验,正向产业化示范线迈进。 相似文献
85.
86.
EGM96和EGM2008地球重力场模型计算弹道扰动引力的比较 总被引:2,自引:2,他引:0
给出了利用地球重力场模型计算扰动引力以及重力场模型截断误差的公式,计算了36阶、360阶、2 160阶地球重力场模型在径向和水平方向的截断误差,并对36阶、360阶的EGM96和EGM2008重力场模型计算的弹道扰动引力矢量进行了分析和比较.结果表明,采用重力场模型计算扰动引力时,模型阶数和弹道点的高度越低,模型截断误差越大;EGM96和EGM2008模型的一致性随着模型阶数的升高而逐渐降低.总体来看,EGM2008模型的精度要好于EGM96模型. 相似文献
87.
Yunquan Li Silin Wu Shi Shu Yiyan Lv Yan Cui 《Marine Georesources & Geotechnology》2020,38(5):604-610
AbstractWith the growing demand for underwater refill engineering, the construction technology of implementing fluidized solidification in underwater pouring has recently received increased attention. However, the environmental impact of underwater casting on surrounding water bodies, especially when the silt is polluted, is still unclear. In this study, a simulated underwater pouring test and a static immersion release test were conducted separately to study the quality of the surrounding water during and after pouring. The results showed that fluidized-solidified silt casting could increase the pH of the surrounding water, even after the pouring had stopped, by approximately 1.0 and the turbidity could reach 200 NTU. No diffusion of Zn and Cu into the surrounding water was observed and while Ni and Cr exhibited slight diffusion, this was at a level well below international water quality standard. During construction, reducing the distance from the seafloor to the bottom of the pouring catheter could reduce the level of pollution. After construction, the use of neutral solidified materials can effectively reduce the pH of the surrounding water and the potential risk of heavy metal dissolution. 相似文献
88.
Zhi-Jia Tian Xiao-Wei Liu Hai-Bo Yuan Bing-Qiu Chen Mao-Sheng Xiang Yang Huang Chun Wang Hua-Wei Zhang Jin-Cheng Guo Juan-Juan Ren Zhi-Ying Huo Yong Yang Meng Zhang Shao-Lan Bi Wu-Ming Yang Kang Liu Xian-Fei Zhang Tan-Da Li Ya-Qian Wu Jing-Hua Zhang 《天文和天体物理学研究(英文版)》2018,(5)
Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy. 相似文献
89.
Understanding the temporal variations of extreme floods that occur in response to climate change is essential to anticipate the trends in flood magnitude and frequency in the context of global warming. However, long-term records of paleofloods in arid regions are scarce, thus preventing a thorough understanding of such events. In this study, a reconstruction of paleofloods over the past 300 years was conducted through an analysis of grain sizes from the sediments of Kanas Lake in the Altay Mountains of northwestern China. Results showed that grain parameters and frequency distributions can be used to infer possible abrupt environmental events within the lake sedimentary sequence, and two extreme flood events corresponding to ca. 1736–1765 AD and ca. 1890 AD were further identified based on canonical discriminant analysis(CDA) and coarse percentile versus median grain size(C-M) pattern analysis, both of which occurred during warmer and wetter climate conditions by referring to tree-ring records. These two flood events are also evidenced by lake sedimentary records in the Altay and Tianshan mountains. Furthermore, through a comparison with other records, the flood event from ca. 1736–1765 AD in the study region seems to have occurred in both the arid central Asia and the Alps in Europe, and thus may have been associated with changes in the North Atlantic Oscillation(NAO) index. 相似文献
90.
This paper systematically studies the rolling effects of the (n, n) single-wall carbon nanotubes (SWCNT) with different curvatures on Rh adsorption behaviours by using density functional theory. The outside charge densities of SWCNTs are found to be higher than those inside, and the differences decrease with the increase of the tube radius. This electronic property led to the discovery that the outside adsorption energies are higher than the inside ones, and that the differences are reduced with the increase of the tube radius. Partial density of states and charge density difference indicate that these strong interactions induce electron transfer between Rh atoms and SWCNTs. 相似文献