花尾胡椒鲷养殖群体遗传多样性的等位酶研究

应用聚丙烯酰胺凝胶电泳（PAGE）技术对取自厦门火烧屿海水网箱的花尾胡椒鲷人工鱼养殖群体的遗传多样性进行等位酶分析。结果表明：所检测的16种同工酶的24个基因座位中，在XDH和GCDH基因座位上发现多态性，其平均多态位点比例为8．33％，基因座位有效等位基因的平均数Ne为1.083，平均杂合度的观测值Ho为0．00556，预期值He为0．00537,Hardy-Weinberg遗传偏离指数（D)为0．035。与其他鱼类相比较说明了其遗传多样性处于较低水平，文中对此进行了探讨并提出相应的种质资源管理保护措施。     

冲绳海槽北部西坡的古三角洲沉积

自20世纪70年代我国开展东海海洋地质调査以来,在海底经常能发现河流、河口沉积物以及与水道相似的负地形、称为古河道、古三角洲或古河口等(李全兴,1990；金翔龙,1992；石斯器等,1989；秦蕴珊等、1987)第四纪气候频繁波动，造成陆架上尤其是外陆架上陆相地层与海相地层频繁交替,因此有不同时期的古三角洲和古河道保留,沉溺的古河道在我国陆架海底的不少地区均有发现。由于海底沉积作用的不均衡性,埋藏较浅的沉溺河谷从地形特征上依稀可辨,埋藏较深的在海底表面则渺无踪迹,只有借助于高分辦率的地球物理测量,才能发现其踪迹。 晚更新世末期，长江、黄河是否流经东海陆架区进入冲绳海槽,虽有不少作者论述,但终因缺少系统的实测资料而未取得共识。长江、黄河在历史上每年向大海输入巨量泥沙,加快了海淤积和推进速度,但古长江三角洲也应该是体积大、构造特征明显的大型堆积体,然而至今尚未有这方面的报道。 作者对冲绳海槽北部进行大面积高分辨率浅地层剖面分析中,发现在陆架外缘坡折线附近,近海底表面处存在大面积斜层理三角洲沉积结构,这为晚更新世末期以来自我国的大河(黄河/长江)从冲绳海槽入海提供了新的证据。     

人工饲料饲养的对虾肠道菌群和水体细菌区系的研究

对人工饲料饲养的凡纳滨对虾Litopenaeus vannamei成虾肠道菌群组成及其水体细菌区系组成进行了研究.分离纯化后的革兰氏阴性菌鉴定到种的水平.对虾肠道菌群主要由弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、嗜氢菌属Hydrogenophaga spp.、伯克霍尔德氏菌Burkholderia spp.、气单胞菌Aeromonas spp.、食酸菌Acidovorax spp.和芽孢杆菌Bacillus spp.组成.水体细菌区系主要包括弧菌Vibrio spp.、希瓦氏菌属Shewnella spp.、艾肯菌Empedobacter spp.、无色杆菌Achromobacter spp.和芽孢杆菌Bacillus spp..肠道菌群和水体细菌区系的优势菌是副溶血弧菌Vibrio parahaemolyticus和芽孢杆菌Bacillus spp..利用Shannon-Wiener指数进行多样性分析,发现虾肠道多样性指数（H）和均匀度（J）高于水体细菌区系,而丰度（D）较低.     

Analysis of Dynamic Characteristics of Submarine Free Spanning Pipelines by Complex Damping Method

Considering the effect of the internal flowing fluid and the external marine environmental condition, the differential equation for the vortex-induced vibration (V1V) of the free spanning pipeline is derived and is discretized by the Hermit interpolation function. The free vibration equation with the damping term is solved by the complex damping method for the natural frequency, and then the effect of fluid damping on the natural frequency of the free spanning pipeline is analyzed.The results show that fluid damping has a significant influence on the damped natural frequency of the free spanning pipeline in the lock-in state, while it has little influence when the pipeline is out of the lock-in state. In the meantime,the change of the free span length has the same effect on the damped natural frequency and the undamped natural frequency.     

Spatial and Temporal Variations of Sound Speed at the PN Section

Gridded sound speed data were calculated using Del Grosso's formulation from the temperature and salinity data at the PN section in the East China Sea covering 92 cruises between February 1978 and October 2000. The vertical gradients of sound speed are mainly related to the seasonal variations, and the strong horizontal gradients are mainly related to the Kuroshio and the upwelling. The standard deviations show that great variations of sound speed exist in the upper layer and in the slope zone. Empirical orthogonal function analysis shows that contributions of surface heating and the Kuroshio to sound speed variance are almost equivalent. This revised version was published online in July 2006 with corrections to the Cover Date.     

Roles of Continental Shelves and Marginal Seas in the Biogeochemical Cycles of the North Pacific Ocean

Most marginal seas in the North Pacific are fed by nutrients supported mainly by upwelling and many are undersaturated with respect to atmospheric CO₂ in the surface water mainly as a result of the biological pump and winter cooling. These seas absorb CO₂ at an average rate of 1.1 ± 0.3 mol C m⁻²yr⁻¹ but release N₂/N₂O at an average rate of 0.07 ± 0.03 mol N m⁻²yr⁻¹. Most of primary production, however, is regenerated on the shelves, and only less than 15% is transported to the open oceans as dissolved and particulate organic carbon (POC) with a small amount of POC deposited in the sediments. It is estimated that seawater in the marginal seas in the North Pacific alone may have taken up 1.6 ± 0.3 Gt (10¹⁵ g) of excess carbon, including 0.21 ± 0.05 Gt for the Bering Sea, 0.18 ± 0.08 Gt for the Okhotsk Sea; 0.31 ± 0.05 Gt for the Japan/East Sea; 0.07 ± 0.02 Gt for the East China and Yellow Seas; 0.80 ± 0.15 Gt for the South China Sea; and 0.015 ± 0.005 Gt for the Gulf of California. More importantly, high latitude marginal seas such as the Bering and Okhotsk Seas may act as conveyer belts in exporting 0.1 ± 0.08 Gt C anthropogenic, excess CO₂ into the North Pacific Intermediate Water per year. The upward migration of calcite and aragonite saturation horizons due to the penetration of excess CO₂ may also make the shelf deposits on the Bering and Okhotsk Seas more susceptible to dissolution, which would then neutralize excess CO₂ in the near future. Further, because most nutrients come from upwelling, increased water consumption on land and damming of major rivers may reduce freshwater output and the buoyancy effect on the shelves. As a result, upwelling, nutrient input and biological productivity may all be reduced in the future. As a final note, the Japan/East Sea has started to show responses to global warming. Warmer surface layer has reduced upwelling of nutrient-rich subsurface water, resulting in a decline of spring phytoplankton biomass. Less bottom water formation because of less winter cooling may lead to the disappearance of the bottom water as early as 2040. Or else, an anoxic condition may form as early as 2200 AD. This revised version was published online in July 2006 with corrections to the Cover Date.     

长江口海水比重的直接测定

海水的比重是海水的一个重要物理性质,也是研究海水运动所必需的一个参数。关于大洋海水比重及其与氯度、盐度和电导的关系已有许多报导[3,5-9],为了制定新的国际海洋学常用表,Cox等人对世界各海洋表层海水的比重、盐度和温度的关系又进行了重新研究[3]。他们指出,除去低盐海水以外,所得结果与Knudsen水文表数据吻合较好,但Knudsen水文表的比重数据平均偏低0.006σ_t。     

一种基于BP算法学习的小波神经网络

为发展 Szu的基于信号表示的小波神经网络 ,提出一种多输入多输出的小波网络模型 ,网络隐层采用框架小波函数、输出层采用 Sigmoid激励函数 ,并选用“熵误差函数”以加速网络的学习速度。奇偶判别和混沌时间序列预测例子的实验结果表明了它具有良好的函数逼近能力和推广能力 ,收敛速度和均方误差均优于相同结构的多层感知器模型。     

现场海域水平扩散系数的探讨 Ⅱ．黄河三角洲埕北海域的计算

根据Ｔａｙｌｏｒ的湍流扩散理论和Ｈａｙ、Ｐａｓｑｕｉｌｌ提出的拉格朗日流和欧拉流的相关函数的相似性，用黄河三角洲埕北海域的实测资料，按四种不同方案计算出该海域的水平扩散系数和谱密度分布。指出：该海区潮流扩散系数主要受潮流的影响，其分布特征与潮流的分布基本一致，所求的水平扩散系数主要是由周期性的潮运动给予潮流扰动所致。方案４是通过富氏变换滤波消去了周期性潮流扰动，本方案计算的水平扩散系数是由周期为６ｈ以下的小尺度涡提供能量。     

中国对虾人工诱导雌核发育的研究 Ⅱ.紫外线辐射对精子顶体反应和受精能力的影响

研究了 36 5 nm波长紫外线辐射中国对虾精子对其顶体反应和受精能力的影响。结果表明 ,低剂量紫外线辐射促进精子发生顶体反应 ,大剂量辐射使精子丧失发生顶体反应的生理机能并死亡。 36 5 nm波长紫外线能够透过精荚和纳精囊盖 ,对其内精子起到杀伤作用。精液稀释液在紫外线下辐射 5～ 8s,仍有部分精子具有受精能力 ,但胚胎在早期阶段即死亡。人工诱导雌核发育的过程中 ,紫外线辐射精液稀释液 5～ 8s,可获得遗传物质失活的精子 (激活源 )。经透射电镜观察分析 ,紫外线对精子遗传物质的损伤是一种使染色质变性的化学作用。