泥蚶(Tegillarca granosa)血红蛋白基因(Tg-HbIIA)克隆、分析及免疫表达研究

通过已构建的泥蚶血液cDNA文库,克隆得到泥蚶Tg-HbIIA基因全长cDNA序列,对其进行生物信息学、组织表达及免疫相关分析。结果表明,泥蚶Tg-HbIIA cDNA全长731bp,包含63bp5′非编码区(5′-UTR),246bp3′非编码区(3′-UTR),开放阅读框450bp,编码150个氨基酸;其氨基酸序列与毛蚶(Scapharca kagoshimensis)和不等壳毛蚶(Scapharca inaequivalvis)的序列有较高的同源性,分别达到89%和88%;对其结构预测显示该蛋白具有血红蛋白功能域,含有10个潜在血红素结合位点。qRT-PCR检测结果显示:泥蚶不同组织部位中,Tg-HbIIA mRNA在血液表达量最大,其次为外套膜和鳃,表达量最低的是肝胰脏;在副溶血弧菌(Vibrio parahaemolyticu)、脂多糖、肽聚糖刺激后,泥蚶血液Tg-HbIIA mRNA表达量显著上调,表明Tg-HbIIA在贝类抗菌免疫防御中可能起重要作用,而Tg-HbIIA对不同致病因子免疫反应的灵敏度和表达时序存在一定差异。     

Walk-away VSP和3D-VSP自适应静校正方法研究

目前Walk-away VSP和3D-VSP处理中尚缺乏有效的静校正手段,主要是借用面地震静校正量.本文根据Walk-away VSP和3D-VSP观测系统和数据特点提出了自适应静校正的思路.主要包括自适应高程静校正和自适应剩余静校正,自适应高程静校正的要点是通过VSP初至时间和激发点高程差,求出替换速度,然后根据高程...     

Characteristics and Genetic Analysis of the Deep-buried Weathered-crust Karst Hydrocarbon Reservoirs of the Lower Paleozoic Group in the Tarim Basin

The genetic analysis of the deep-buried reservoirs of the Lower Paleozoic carbonate rocks in the Tarim basin is a difficult task involving many factors. Firstly, the object of study is carbonate rocks, which have undergone a long term of modification. Secondly, the rocks are deeply buried with depths of 3800-7000 m in the Tarim basin. The primary reservoir properties formed in the deposition have been strongly modified during the deep burial process. Concurrently, the different burial depths in different areas result in diversities of burial temperature, pressure, underground water, hydrochemistry and various physicochemical changes, which further lead to differences in the diagenetic type, diagenetic property, diagenetic degree and their impacts on the reservoir properties. The Lower Paleozoic Cambrian and Ordovician carbonate reservoirs in the Tarim basin can be grouped into four types, i.e., paleo-weathered-crust reservoirs, reef reservoirs, buried karst reservoirs and dolomite reservoirs. This paper     

Geographical distribution of ecological footprint and sustainability analysis for Liaoning Province

This paper presents the detailed results and analyses on the ecological footprints and bio-capacities of the individual cities and the province as a whole for the year 2001, providing a clear picture of sustainability for the province. Results show that the ecological footprints of most cities in Liaoning exceeded their respective bio-capacities, incurring high ecological deficits. The ecological deficit of the province as a whole was 1.31 ha/cap. Those cities with resources extraction and/or primary material-making as their major industries constitute the "ecologically black band", whose ecological deficits ranged from 2.45 to 5.23 ha/cap, the highest of all cities in the province. Fossil energy consumption was the major source of footprint amounting to 1.63 ha/cap at the provincial level, taking up 67.3% of the total. For cropland, modest ecological surpluses occurred in Jinzhou, Tieling, Huludao, and Panjin while modest ecological deficits in Dalian, Benxi, Fushun, and Dandong, resulting in an overall surplus for the province. Liaoning had a certain level of surplus in fishing ground (water area), mainly distributed in the coastal cities of Dalian, Panjin, Huludao, Yingkou, Jinzhou, and Dandong. Most cities had a small ecological deficit in pasture and all had a small ecological surplus in forest. The eco-efficiency, expressed as GDP value per hectare of footprint, exhibits high variations among the cities, with the highest (Shenyang) more than 10 times the lowest (Fuxin). Cities with manufacture, high-tech, and better developed service industries had high eco-efficiency, while those with resources extraction, primary material-making, and less developed service industries had low eco-efficiency. Based on the components and geographical distribution of ecological footprint, strategic policy implications are outlined for Liaoning's development toward a sustainable future.     

山东饮用天然矿泉水及其勘查与保护

山东是饮用天然矿泉水资源大省 ,点之多、量之丰居全国前列。到目前为止 ,已鉴定矿泉水 36 1处 ,其中锶型118处 ,偏硅酸型 6 4处 ,锶偏硅酸型 15 1处 ,碘型等其它类型 2 8处。这些矿泉水所赋存的岩石类型多、岩性复杂且受断裂构造控制明显 ;具有达标元素较单一、水化学类型复杂、矿化程度较低、单井允许开采量相差悬殊且地域分布不均等特点。勘查与评价重点是寻找奇缺且又可能存在的锌型、锂型、碳酸型矿泉水。开发好矿泉水资源、实行规模开发、打造本省名牌、加强矿泉水资源的卫生防护与保护 ,对于确保山东矿泉水资源的持续开发与利用具有重要意义。     

水动力作用下管道稳定性的试验研究

利用振荡流水槽在以下两种约束条件下，研究波浪作用下直接铺设于砂质海底的管道失稳临界条件：1）管道两端自由；2）管道可水平、垂直自由运动，但流动受到限制。试验结果表明，管重无量纳数G与管道失稳的临界Fr数之间大致呈线性关系，而当G超过一定数值时，管道是侧向稳定的。管道的约束条件、砂床特性和加载速度对管道稳定性均有影响。     

一个土墩日珥的D3线光谱分析Ⅰ-D3线特征及其计算

198 4年 5月 5日太阳东边缘土墩日珥的Hα单色像和D3线被观测到了。D3发射线由两部分组成 :主成分和致宽成分。本文利用双层模型方法对该土墩日珥的 1 7条D3发射线成功地进行了计算 ,给出了D3线的计算结果。计算结果表明 :日珥的D3发射线主要由热的多普勒致宽和微观湍流致宽所致 ,其它致宽机制的作用可以忽略     

海上液货补给技术

在详细研究大量国内外有关海上液货补给技术资料的基础上,简单论述了液货补给装置发展的历史与现状,并分析各类方法的优缺点,提出可以改进的方法。     

广东近岸海湾表层沉积物的稀土元素特征及其物源示踪

本文对广东省近岸10个典型海湾的表层沉积物进行了系统的稀土元素(rare earth elements,REE)测试和分析,以期发现沉积物的物源组成特征及其控制因素。研究结果表明,同一海湾内沉积物的稀土丰度差异不明显,但不同海湾之间具有一定的差别(133.58—251.77mg.kg 1)。球粒陨石标准化配分曲线表现出轻稀土(La到Eu)富集、重稀土(Gd到Lu)亏损、δEu负异常等典型的陆源沉积物特征;PAAS(Post-Archean Australian Shale)标准化配分模式显示出明显的中稀土(Sm到Ho)富集特征,与已报道的国内河流沉积物REE特征相似。海湾沉积物与入海河流沉积物及流域内的原岩组成特征的比较说明,后者对海湾沉积物的控制作用明显。REE配分曲线的一致性和中稀土富集特征表明海湾沉积物主要来自汇入海湾的山溪及河流输入的沉积物。     

洋山深水港区海域秋、冬季沉积物中重金属来源分析及生态风险评价(英文)

通过测定洋山深水港区海域2010~2013年6个航次秋、冬季沉积物中重金属含量,分析其时空差异,结果表明该海域沉积物重金属总体含量较低,空间分布较为均匀,Hg、Zn、Pb、Cd含量秋季明显高于冬季,As、Cu含量的季节性波动较小,用因子分析法研究该海域重金属来源,发现陆源工业、船舶航运排污以及有机质降解是研究海域Pb、Cd、Zn的主要来源;农业污染、码头货物装卸残留及建筑垃圾支配着Cu、As、Hg的来源。SQG-Q生态风险评价表明研究海域沉积物中重金属均存在中、低度的生态风险,秋季的生态风险高于冬季,Hg和Cu是主要生态风险因子;地累积指数法评价结果显示研究海域基本不受Hg、As、Zn、Pb的污染;Cd、Cu以轻度污染为主,部分站位属于偏中度污染,6种重金属污染程度依次为:CdCuPbZnHgAs。因子综合得分评价表明,重金属污染相对严重的站位依次为5号、4号及3号站位。但总体看来洋山深水港区附近海域沉积物中重金属污染状况属于轻度污染。