合浦珠母贝卵子电击脉冲参数的初步研究

运用电脉冲转基因仪对合浦珠母贝卵子进行电脉冲处理以优化电穿孔法转基因的电击参数。实验中调制百分比：100％；脉冲间隔：1．0s：电极距离：2mm；脉冲个数：5个，参数保持不变。用不同的电脉冲参数组合（脉冲电压100-400V、脉冲持续时间0．5～3．0ms、脉冲频率10～50kHz，共28个组合）对卵子电击，然后加入精子进行授精，统计各组受精率和孵化率，以受精率和孵化率达到50％以上为衡量指标。结果表明，合浦珠母贝卵子对低脉冲频率和高电压非常敏感，当频率低于20kHz或脉冲电压高于400V时对卵子损伤较大。脉冲持续时间对其影响不大，适应范围较宽。优化的转基因电击脉冲参教组合为：脉冲电压1.0kV／cm，脉冲持续时间20ms，脉冲频率50kHz，或者脉冲电压1．5kV／cm，脉冲持续时间1．5ms，脉冲频率50kHz。     

桩式离岸堤保滩促淤工程消浪效果试验研究

在上海奉贤南北港保滩促淤工程中，采用了一种新型结构型式－桩式离岸堤，并通过物理模式试验进行了桩式离岸堤消浪效果研究。针对离岸堤通常建于近岸水区破波带的特点，重点研究水深，堤高以及堤身结构对波浪衰减的影响，同时对桩式离岸堤堤后水域的波浪底流速 分析探讨。研究结果表明，桩式离岸堤不仅具有良好的消浪效果。而且可在较大范围内改变波态，即由引起水体剧烈紊动的破波转变为浅水推进波，从而有效地改善海滩上的动力条件，促进海滩免受侵蚀，是一种具有广泛应用前景和新型保滩促淤结构。     

Numerical Simulation of Wave Field near Submerged Bars by PLIC-VOF Model

Investigating the wave field near structures in coastal and offshore engineering is of increasing significance. In the present study, simulation is done of the wave profile and flow field for waves propagating over submerged bars, using PLIC-VOF （Pieeewise Linear Interface Construction） to trace the free surface of wave and finite difference method to solve vertical 2D Navier-Stokes （N-S） equations. A comparison of the numerical results for two kinds of submerged bars with the experimental ones shows that the PLIC-VOF model used in this study is effective and can compute the wave field precisely.     

山东省海岸工程可持续发展的若干问题

从风暴潮、黄河口治理、海水地下入侵与咸水侵染、海岸及沿海污染、沿海滩涂与黄河三角洲开发、海上交通和港口发展、海岸和海上石油开采、海岸工业基地建设、沿海旅游和海岛开发 ,以及沿岸淡水资源开发与海水利用等 1 0个方面 ,对山东省海岸工程的可持续发展问题进行了分析和讨论 ,并针对具体问题 ,提出了解决可持续发展问题的对策与措施。     

Comparison of Drags on Fish Cages of Different Shapes

1 .Introduction Commercial fishfarming in net cages is becoming widespread around the world as an importantsource of food.In PRChina ,mostfishfarmingtakes placeincalmcoastal areas .Fishcages work wellat these sites becausethe environmental forces are usua…     

孔鳐胰脏单层细胞的制备和外源基因的表达

采用机械法、酶消化法和灌注冷消化结合机械法对孔鳐胰脏细胞进行分散,并以台盼蓝染色法测定活细胞率,评价细胞分散后的生长状态.在细胞分散后对孔鳐胰脏细胞用腺病毒载体和阳离子多聚物进行外源基因的转染.实验结果表明,灌注冷消化结合机械法可以获得较高的活细胞率,胰脏细胞可以正常生长并表达外源基因.     

养殖石鲽鱼病原菌鸭瘟巴斯德氏菌(Pasteurella anatipestifer)初步研究

1998年7月从寻山水产集团公司养鱼场养殖的石鲽鱼病鱼肠道中分离到多株细菌，经人工感染试验证明其中1株为致病菌。该菌株为革兰氏阴性短杆菌，无动力，32℃以上不生长。氧化酶阳性。发酵葡萄糖产酸不产气。经BIOLOG MICROSTATION SYSTEM鉴定为鸭瘟巴斯德氏菌（Pasteurella anatipestifer)。药敏试验表明，该菌株对呋喃唑酮、呋喃妥因、氟哌酸、丁胺卡那和环丙沙星敏感。     

The algorithms of chlorophyll-a concentration for CMODIS

The algorithms of extracting chlorophyll-a(Chl-a) concentration have been established for Chinese moderate resolution imaging spectrometer(CMODIS) mounted on Shenzhou-3 spaceship launched on 25 March 2002.The CMODIS is an ocean color sensor with 30 visible channels and 4 infrared channels,much different from other ocean color satellites and needs new algorithms to process data.Three models of Chl-a concentration were established based on Chl-a data retrieved from sea-viewing wide field-of-view sensor(SeaWiFS),with the average relative errors of 26.6%,24%.0% and 33.5%,respectively.This practical and economic approach can be used for developing the algorithms of Chinese ocean color and temperature sensor(COCTS) on the satellite Haiyang-1 to derive the Chl-a concentration concentration distribution.The applicability of the algorithms was analyzed using some in situ measurements.Suspended sediment is the main factor influencing the accuracy of the spectral ratio algorithms of Chl-a concentration.The algorithms are suitable to using in the regions where suspended sediment concentrations(SSC) are less than 5 g/m3 under the condition of relative error of Chl-a concentration retrieval within 35%.High concentration of suspended sediment leads to the overestimate remote sensing retrieval of concentration of Chl-a,while low-middle SSCs lead to the low Chl-a concentration values using the spectral ratio algorithms.Since the accuracy of Chl-a concentration by the spectral ratio algorithms is limited to waters of Case 2,it is necessary to develop semi-analytical models to improve the performance of satellite ocean color remote sensing in turbid coastal waters.     

电磁波信号在对流层中路径弯曲改正的级数展开式

大气折射延迟是空间大地测量中的一个主要误差源。本文严格地给出了中性大气路径弯曲改正的原理性公式。通过对各种因素量级的分析,在亚毫米量级的精度上给出了一个以视天顶距正切和正割乘积为参数的级数展开模型。新模型在球对称大气模型下,级数展开模型的精度达到了毫米量级,理论上可以展开到任意阶次,且可允许观测高度角小到5°。     

基于ArcEngine的虚拟校园信息管理系统的设计与实现

阐述基于组件式GIS的开发方式,结合3维建模技术、数据库技术开发虚拟校园信息管理系统的基本思路,重点研究了3维模型的入库方法,以及基于GeoDatabase的数据库设计。以VirtualCampus系统开发为例,介绍了利用Visual C++与ArcEngine开发虚拟校园信息管理系统的方法。