西太平洋深海海水中乙醛降解菌多样性及群落结构研究

As a major aldehyde pollutant widely existing in industry and our daily life, acetaldehyde is more and more harmful to human health. As characteristic habitat niche, bacteria from deep sea environments are abundant and distinctive in heredity, physiology and ecological functions. Thus, the development of acetaldehyde-degrading bacteria from deep sea provides a new method to harness acetaldehyde pollutant. Firstly, in this study,acetaldehyde-degrading bacteria in the deep sea water of the West Pacific Ocean were enriched in situ and in the laboratory respectively, and then the diversity of uncultured bacteria was studied by using 16 S r RNA genes. Then acetaldehyde-degrading strains were isolated from two samples, including enrichment in situ and enrichment in laboratory samples of deep sea water from the West Pacific Ocean using acetaldehyde as the sole carbon source,and then the ability of acetaldehyde degradation was detected. Our results showed that the main uncultured bacteria of two samples with different enrichment approaches were similar, including Proteobacteria,Actinobacteria, Firmicutes, Cyanobacteria, but the structure of bacterial community were significant different.Four subgroups, α, γ, δ and ε, were found in Proteobacteria group. The γ-Proteobacteria was dominant(63.5%clones in laboratory enriched sample, 75% clones in situ enriched sample). The species belonged to γ-Proteobacteria and their proportion was nearly identical between the two enrichment samples, and Vibrio was the predominant genus(45% in laboratory enriched sample, 48.5% in situ enriched sample), followed by Halomonas(9% in situ enriched sample) and Streptococcus(6% in laboratory enriched sample). A total of 12 acetaldehyde-degrading strains were isolated from the two samples, which belonged to Vibrio, Halomonas,Pseudoalteromonas, Pseudomonas and Bacillus of γ-Proteobacteria. Strains ACH-L-5, ACH-L-8 and ACH-S-12,belonging to Vibrio and Halomonas, have strong ability of acetaldehyde degradation, which could tolerate 1.5 g/L acetaldehyde and degrade 350 mg/L acetaldehyde within 24 hours. Our results indicated that bacteria of γ-Proteobacteria may play an important role in carbon cycle of deep sea environments, especial the bacteria belonging to Vibrio and Halomonas and these strains was suggested for their potentials in government of aldehyde pollutants.     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

基于Hadoop的地理国情监测高性能计算分析研究

随着常态化地理国情监测在我国的全面开展,地理国情时空数据规模不断增大,使得对地理国情数据综合分析和信息挖掘的要求越来越高。本文针对传统空间数据存储和分析平台存在的性能瓶颈问题,提出了一种基于Hadoop的地理国情高性能计算框架,论述了通过服务封装实现分布式并行计算能力与成熟平台地理空间分析能力的无缝聚合实现最优化地理国情分析计算的理念,并以地理国情地表覆盖面积统计为例,探讨了分布式计算技术在地理国情海量数据分析方面的适用性。结果表明,随着数据量的增加,分布式计算集群的性能优势逐渐凸显,比传统集中式空间数据库更适合于海量数据的分析计算,而集群的良好横向拓展性,也为大规模地理国情空间数据分析提供了可行解决方案,研究结果将为进一步构建开放性地理国情分析服务平台奠定基础。     

中国省域煤矿事故时空分异特征及影响因素研究

煤矿事故给国家和人民带来巨大的生命财产损失,科学把握煤矿事故发生的宏观规律及区域煤矿安全的影响因素有助于促进煤矿安全生产。本文尝试采用GIS空间分析方法开展区域煤矿事故的研究,基于空间自相关性、时空演化规律方法研究了2006-2015年中国省域煤矿事故的时空分异特点,并分析了省域煤矿安全的影响因素及相对风险度。主要结论：①中国各省区之间煤矿事故存在明显的空间自相关性,但随着各省区煤矿安全生产形势改善情况的不同,其自相关性在不断下降;②以空间重心转移曲线分析中国煤矿事故发生的时空演化规律,10年间煤矿事故空间重心向东北方向转移。③重特大事故频发的省区由于监管措施的有力执行往往具有较低的煤矿百万吨死亡率;平均煤矿生产能力低的省区具有较高的煤矿百万吨死亡率;④建立基于解释变量的贝叶斯空间模型,以煤炭百万吨死亡率为指标评估中国各省区煤矿事故相对风险度,中国煤矿安全生产相对危险度存在明显的空间分布规律且各省区差异较大,相对危险度较高的省区主要集中在南方地区,包括湖北、福建等,危险度较低的省区主要集中在中东部地区,包括山西、内蒙古等。     

基于迁移学习方法的山东冷云冰晶特征分布研究

为分析冷云中冰晶的分布特征,揭示冰晶增长演变机制,根据冰晶形状和尺度特征分为8类并进行标注,同时标注1类隔断栏进行数据质量控制,将9类标签图像整合并建立图像集,利用迁移学习VGGNet16方法进行识别训练,经训练模型分类准确率达98%。将模型应用到秋季冷云冰晶特征研究中,选取3次积层混合云和3次层状云降水过程,分析冰晶形状在不同温度区间的占比及冰晶谱变化特征,结果表明,温度通过影响冰晶基面与棱面的比值来决定冰晶初始形状分布,相同温度区间积层混合云内球状冰晶和线型冰晶占比高于层状云,低于-12℃后各类冰晶占比相对固定;积层混合云内线型冰晶直径集中在300~800 μm,冰晶谱呈多峰分布,聚合体直径大于600 μm,冰晶谱首尾两端浓度相当,球状冰晶直径集中在120～300 μm,冰晶谱呈单调下降趋势。     

基于印度洋海温信号的我国西北地区东部夏季降水组合降尺度预测方法研究

利用国家气候中心第二代气候模式预测业务系统(BCC-CPSv2)预测产品,引入印度洋海温信号,采用组合降尺度方法建立了西北地区东部汛期降水预测模型。该预测模型对1991—2017年西北地区东部夏季降水的回报技巧较BCC-CPSv2预测技巧显著提高,空间相关系数由0.42提高到0.75,均方根误差明显减小,最多下降达80%。预测模型对降水空间分布型的预测能力较好,很好地回报了典型年份（1987年和2010年）夏季的降水距平百分率分布。通过抓住气象变量的空间分布特征,组合降尺度方法可以修正动力模式产品的预测误差,为西北地区东部夏季降水预测提供科学依据和技术支持,具有较好的应用前景。     

江南造山带中段地壳结构特征——来自武宁—吉安深反射地震随机介质相关长度分析的认识

江南造山带位于扬子和华夏地块中间,受华南陆块及其周缘多期次的碰撞、裂解及再造过程影响,经历了复杂的构造运动和变形,于中生代期间受区域构造-岩浆事件影响,发生成矿“大爆发”,是研究陆内深部过程、成矿作用的关键地区。受沉积覆盖、出露较少等因素影响,江南造山带中段地壳结构、深部过程及其边界范围等还存在争议。为进一步提高对江南造山带中段中生代深部构造背景、动力学过程及成矿作用的认识,本文利用武宁—吉安深反射地震数据,进行随机介质参数的计算,获得了相关长度剖面。结果发现江南造山带中段地壳厚度沿剖面由北向南逐渐减薄,结合前人研究推测研究区受控于燕山期晚期伸展构造体制,在伸展构造背景下,导致地壳减薄,诱发幔源物质上涌,受地幔上涌影响由南往北逐渐减弱。同时揭示了宜丰—景德镇断裂深大断裂极性,进一步证明了萍乡—广丰断裂为江南造山带中段与华夏地块的边界。最后综合分析了中生代区域构造对成矿的影响,认为在伸展构造背景下,幔源物质沿断裂上涌控制了该区金、钨等矿床的形成。     

内蒙古固阳地区太古宙乌拉山群构造变形分析

乌拉山群的变质－变形问题一直有不同的认识＾「１～３」。该文以固阳地区３幅１∶５万区调资料为基础,结合９０年代以来周边地区１∶５万区调成果,提出晚太古宙乌拉山群经历了两期主要的构造变形。第一期为伸展体制下近水平分层剪切形成的固态流变褶皱和高温顺层韧性剪切带,属高角闪岩相－麻粒岩相,变形时间为太古代末;第二期构造变形为地壳抬升之后的剂压体制下,中、浅层次的紧闭同斜相似褶皱－－主期褶皱和低温韧性剪切带,     

赣南东部银坑-青塘地区上施组硅质白云岩特征及其找矿意义

在赣南东部银坑－－青塘地区广泛分布晚元古代地层,近年普查找矿发现青白口纪上施组地层有两层典型硅质白云岩层,该白云岩层层位稳定,特征明显,并与本地区的铅锌矿床关系密切。本文主要从硅质白云岩层和层控铅锌矿床的一些基本特征入手,阐述了它们之间的相互关系,明确指出上施组硅质白云岩层对寻找富铅锌矿床具重要的意义。     

海勃湾水库用于黄河内蒙古河段防凌作用的研究

黄河内蒙古河段冰期一般在冬季封河期和春季开河期０容易出现严重凌情,造成灾害。故良好的封河形势和开河形势是避免凌灾发生的重要条件,自１９６８年刘家峡水库运用以来,黄河内下河段凌情已大有缓解。但是,由于有峡水库距黄河内下河段较远。对河道凌情变化反应不够灵敏。因此,一些年份仍有较为严重的凌灾发生。在分析黄河内蒙古河段封、开河形势特点的基础上,深入研究了海勃湾水库用于该段春季防凌调度的作用和能力,为黄河内