山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

Characteristics of Scour and Deposition in Front of Breakwaters Under Irregular Broken Clapotis

First the scour and deposition patterns of the sandy seabed in front of a vertical breakwater under the action of irregular broken clapotis are investigated experimentally and classified into five types: scour type Ⅰ , scour type Ⅱ, scour type Ⅲ, deposition type Ⅰ, and deposition type Ⅱ . Secondly, the processes of formation of scour and deposition patterns are probed in comparison with those induced by regular broken clapotis and standing waves. Thirdly, the criteria for distinguishing scour and deposition patterns under irregular broken clapotis are presented.     

河口环流和盐水入侵Ⅱ--径流量和海平面上升的影响

本文利用本序列上篇论文建立的理想河口数值模式,研究径流量和海平面上升对河口环流和盐水入侵的影响.在径流量增大的情况下,口门内表层向海的流速增大,底层向陆的密度流减弱,滞流点下移.口门外侧向口门的密度流增大,上升流趋于增强.口门内盐水入侵减弱,口外盐度减小、冲淡水扩展范围增大.在口门上游北岸底层盐度下降明显,口门处南岸表层盐度下降明显.径流量变化对盐水入侵影响十分巨大.在海平面上升的情况下,拦门沙区域向陆的密度流增强,滞流点上移,表层向海的流动增大.口门内盐水入侵增强,口外盐度增大,冲淡水扩展范围减小.海平面上升对盐水入侵影响十分明显,北岸底层盐度增大尤为特出.     

多波束数据的海底数字地形模型构建

提出大批量多格式原始多波束数据的DTM构建方法，以满足大区域、大比例尺海底地形制图的需要。在多种格式原始多波束数据接口和系统内部标准数据结构的基础上，通过对数据文件、数据种类和数据运算量的有效组织管理，实施边读入、边权重配赋的网格插值，分析了高斯、指数和距离平方反比权重函数的适用性，及最小值、最大值和平均值的实用性。在权重配赋网格插值基础上，提出分形fBM和张力样条配合使用的方程式插值方法，保证DTM数据的有效外延和地形分辨。整套算法效率高，并能有效保证DTM的精度，对存储在外部介质的数据遍历一次即可完成网格插值。     

Modeling of Multi-Freedom Ship Motions in Irregular Waves with Fuzzy Neural Networks

1 .IntroductionAshiptravelingatseaundergoesundesirablewave inducedmotions ,namely ,surge ,sway ,heav ing ,rolling ,pitchingandyaw .Thesemotionsoftencauseproblemstothecrew ,theonboardequip mentand ,intheworstcase ,thesafetyofthevessel.Tominimizethewave inducedshipmotions ,controlsystemsmaybeapplied .Theaccuratemodelingofshipmotionsisthereforeveryimportantforshipdesignanddesignofmotioncontrolsystems .Manyresearchershavedevelopedshipmotionpredictionmethodsbasedonthepotentialflowtheo ries (Dong ,…     

河口Si输运过程研究

基于拉格朗日余流及其输运过程的一种三维空间弱非线性理论，进一步假定Ｓｉ在河口的无因次化学转移项量级为ｋ＾２，给出了Ｓｉ的长期输运方程。对于一种二维模型河口，数值求解了零阶天文潮，欧拉余流、斯托克斯漂移、拉格朗日余流和盐度的分布；分别计算了平均逼留时间为１３，３０，６０，１８０ｄ等所对应的Ｓｉ浓度分布；给出了Ｓｉ－ｓ相关图，讨论了河口中Ｓｉ的保守性问题。     

一种灰树花菌丝体多糖GF4F1的结构特征及其抗肿瘤活性

以灰树花发酵菌丝体为原料，经过提取分离纯化得到1种水溶性多糖GF4F1，经高碘酸氧化、Smith降解、甲基化、红外光谱（IR）以及核磁共振波谱（NMR）等分析表明，该多糖是1种β-1, 3／1，6-葡聚糖，且小鼠体内实验结果表明其具有良好的抗肿瘤活性。在1mg／kg时，对小鼠抑制率为67.3％。     

南海黄鲷性腺发育的初步研究

本文采用常规的生物学调查方法并取黄鲷性腺做石蜡切片，用显微镜观察黄鲷的性腺发育，进行组织学研究．其结果表明，南海黄鲷精巢可分成6期，精巢5期的成熟度指数为1．62％～2．71％，平均值为1．99％．雌性生殖细胞可见5个时相，卵巢发育也相应分成6期，卵巢5期时的成熟度指数为8．90％～13．90％，平均值为9．45％．南海黄鲷的繁殖期在11月至翌年的4月，繁殖高峰在12月至3月，黄鲷的产卵类型属于在同一繁殖季节中多次产卵类型．本文还对黄鲷的雌雄同体现象、繁殖力、繁殖时间进行了讨论．     

分形分析方法在海冰趋势预测中的应用

根据渤海北部1966——2001海冰冰级年际变化的实际资料，利用R／S分析的原理和方法计算了H指数，建立了R(i)/S(i)与i的关系式，对渤海北部海冰趋势进行了预测分析。结果表明，利用分形分析方法进行海冰趋势预测不仅方法简便，而且结论具有客观性，可以将其作为海冰趋势预测中较为有效的一种方法。     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.