4-tert-octylphenol and 17beta-estradiol applied by feeding to flounder Platichthys flesus: induction of vitellogenin and accumulation in tissues

In order to simulate a possible natural administration route of xenoestrogens male flounder Platichthys flesus were exposed via the diet to the alkylphenol 4-tert-octylphenol. Treatment with 4-tert-octylphenol (10, 50 and 100 mg OP kg(-1) BW) or 17beta-estradiol (0.05 mg kg(-1) BW) every second day during a period of 11 days resulted in a significant increase in plasma vitellogenin (Vtg) concentrations. The induction of Vtg was greatest in the fish receiving 50 mg OP kg-' BW. A significant accumulation of 4-tert-octylphenol was found in liver and muscle tissue of the OP treated groups. The tissue concentrations of 4-tert-octylphenol and the plasma vitellogenin concentration were positively correlated. The results show that 4-tert-octylphenol accumulates in liver and muscles of flounder P. flesus, and exerts estrogenic effects such as vitellogenin induction.     

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.     

太平洋西南部铁锰矿化的矿物学特征

太平洋西南部铁锰矿化的矿物学特征Ａ．Ｈ．Ｋａｎ等在对克马德克和新赫布里底岛弧—海沟构造系不同地貌构造的地质采样过程中（１９９０年科学考察船“ＡＨｅｃＭＨｏＢ院士”号第１７航次），在一系列测站上采集到不同的铁锰形成物（ＭＯ）。详细研究这些形成物具有特殊...     

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。     

Improved form of wind wave frequency spectrum

The lower frequency part of the theoretical wind wave spectrum proposed by the authors (Wen et al. , 1988a, b,c) has been improved and the form of spectrum is appreciably simplified. In addition to the field data collected in the Bohai Sea region and used in the previous papers, those obtained in the Huanghai Sea, the East China Sea and the South China Sea have been employed so that the improved spectra can be verified on a more extensive observational basis. Computed results agree with the observations well. Further comparisons have been made between the proposed spectra and the JONSWAP spectrum. Though the two types of spectrum are close to each other in form, the former shows, as a whole, better agreement with the observation than the latter. By introducing an improved relation between the peak-ness factor and significant wave steepness, the spectrum contains only significant wave height and period as parameters. For spectra given in this form, the computed peak frequencies coincide approximately wit     

Atmospheric Correction of SeaWiFS Data for the Retrieval of Suspended Sediment in East China Coastal Waters

The data of SeaWiFS (Sea-Viewing Wide Field-of-View Sensor), installed on SeaStar, has been used to generate SSC (suspended sediment concentration) of complex and turbid coastal waters in China. In view of the problems of the SeaDAS (SeaWiFS Data Analysis System) algorithm applied to China coastal waters, a new atmospheric correction algorithm is discussed, developed, and used for the SSC of East China coastal waters. The advantages of the new algorithm are described through the comparison of the restdts from different algorithms.     

Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.     

白垩纪温度较冷的估价

白垩纪温度较冷的估价Ｂ．ｗ．Ｓｅｌｌｗｏｏｄ等有关白垩纪气候的概念部分基于详尽的地质证据，如不耐寒冷的古生物（如：珊瑚的分布比现在偏极地５－１５℃）和特殊气候条件的沉积相的广泛分布。这些资料的解释反映２１℃的等温线在纬度上至少向两极移动５°，全球的平...     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po