Experimental investigation of the combustive sound source

In this paper, we describe a unique low frequency underwater sound source called the combustive sound source (CSS). In this device, a combustible gas mixture is captured in a combustion chamber and ignited with a spark. The ensuing combustion produces expanding gases which in turn produce high intensity, low frequency acoustic pulses. With high-speed motion pictures of the CSS event, we relate the motion of the bubble to the acoustic waveform. We also compare the measured first bubble period in the CSS pressure signature with the predictions of the Rayleigh-Willis equation, including the dependence of the radiated acoustic waveform on the volume and depth of the bubble. Measurements of the first bubble period agree with Rayleigh-Willis theory in trend, but not in absolute value. In addition, we discuss the variation of the acoustic output with the fuel/oxygen mixture. Finally, several other factors that affect the acoustic output of CSS are discussed. These include the shape of the CSS combustion chamber, the type of oxidizer and fuel, and the ignition source     

Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Response of the blue mussel Mytilus edulis L. following exposure to PAHs or contaminated sediment

The polycyclic aromatic hydrocarbons fluoranthene and benzo(a)pyrene significantly reduced the feeding rate of mussels. For both compounds the tissue concentration resulting in a 50% reduction of the clearance rate (TEC₅₀) was calculated. At high tissue concentrations both aromatic compounds reduced the tolerance of mussels to aerial exposure, whereas at low tissue concentrations an improved response was noticed. The activities of the antioxidant enzymes Superoxide dismutase (SOD) and catalase were elevated only at low tissue concentrations of fluoranthene and benzo(a)pyrene. At the highest measured tissue concentrations the activity of both enzymes was reduced, possibly due to a narcotic effect. The reproductive success rate of mussels appeared to be affected negatively by the investigated hydrocarbons. The results of a pilot experiment indicate that mussels can be used also for the testing of contaminated sediments.     

Buckling of circular, solid and annular plates with an intermediate circular support

The title problem is solved in the case where uniform applied loading is present at the plate outer boundary. Two independent solutions are obtained: the optimized Rayleigh–Ritz method and the finite element algorithmic procedure. Good engineering agreement is shown to exist. The proposed analytical procedure is convenient from a designer's viewpoint. Admittedly exact analytical solutions can be found in terms of Bessel functions, the procedure being rather complex from an engineering viewpoint.     

Holocene reef growth in Torres Strait

The platform and fringing reefs of Torres Strait are morphologically similar to reefs of the northern Great Barrier Reef to the south, except that several are elongated in the direction of the strong tidal currents between the Coral Sea and the Gulf of Carpentaria. Surface and subsurface investigations and radiocarbon dating on Yam, Warraber and Hammond Islands reveal that the initiation and mode of Holocene reef growth reflect antecedent topography and sea-level history. On the granitic Yam Island, fringing reefs have established in some places over a Pleistocene limestone at about 6 m depth around 7000 years BP. Emergent Holocene microatolls of Porites sp. indicate that the reefs have prograded seawards while sea level has fallen gradually from at least 0.8 m above present about 5800 years BP. On the Warraber Island reef platform drilling near the centre indicated a Pleistocene limestone foundation at a depth of about 6 m over which reefs established around 6700 years BP. Reef growth lagged behind that on Yam Island. Microatolls on the mature reef flat indicate that the reef reached sea level around 5300 years BP when the sea was around 0.8–1.0 m above present. On the reef flat on the western side of Hammond Island bedrock was encountered at 7–8 m depth, overlain by terrigenous mud. A progradational reef sequence of only 1–2 m thickness has built seaward over these muds, as sea level has fallen over the past 5800 years. Reef-flat progradation on these reefs is interpreted to have occurred by a series of stepwise buildouts marked by lines of microatolls parallel to the reef crest, marking individual coalescing coral heads. Detrital infill has occurred between these. This pattern of reef progradation is consistent with the radiocarbon dating results from these reefs, and with seismic investigations on the Torres Reefs.     

Large aperture seismic imaging at a convergent margin: Techniques and results from the Costa Rica seismogenic zone

In March and April 1995 a cooperative German, Costa Rican, and United States research team recorded onshore-offshore seismic data sets along the Pacific margin of Costa Rica using the R/V Ewing. Off the Nicoya Peninsula we used a linear array of ocean bottom seismometers and hydrophones (OBS/H) with onshore seismometers extending across much of the isthmus. In the central area we deployed an OBS/H areal array consisting of 30 instruments over a 9 km by 35-km area and had land stations on the Nicoya Peninsula adjacent to this marine array and also extending northeast on the main Costa Rican landmass. Our goal in these experiments was to determine the crustal velocity structure along different portions of this convergent margin and to use the dense instrument deployments to create migrated reflection images of the plate boundary zone and the subducting Cocos Plate. Our specific goal in the central area was to determine whether a subducted seamount is present at the location of the 1990, M 7 earthquake off the Nicoya Peninsula and can thus be linked to its nucleation. Subsequently we have processed the data to improve reflection signals, used the data to calculate crustal velocity models, and developed several wide-aperture migration techniques, based on a Kirchhoff algorithm, to produce reflection images. Along the northern transect we used the ocean bottom data to construct a detailed crustal velocity model, but reflections from the plate boundary and top and bottom of the subducting Cocos plate are difficult to identify and have so far produced poor images. In contrast, the land stations along this same transect recorded clear reflections from the top of the subducting plate or plate boundary, within the seismogenic zone, and we have constructed a clear image from this reflector beneath the Nicoya shelf. Data from the 3-D seismic experiment suffer from high-amplitude, coherent noise (arrivals other than reflections), and we have tried many techniques to enhance the signal to noise ratio of reflected arrivals. Due to the noise, an apparent lack of strong reflections from the plate boundary zone, and probable structural complexity, the resulting 3-D images only poorly resolve the top of the subducting Cocos Plate. The images are not able to provide compelling evidence of whether there is a subducting seamount at the 1990 earthquake hypocenter. Our results do show that OBS surveys are capable of creating images of the plate boundary zone and the subducting plate well into the seismogenic zone if coherent reflections are recorded at 1.8 km instrument spacing (2-D) and 5 km inline by 1 km crossline spacing for 3-D acquisition. However, due to typical high amplitude coherent noise, imaging results may be poorer than expected, especially in unfavorable geologic settings such as our 3-D survey area. More effective noise reduction in acquisition, possibly with the use of vertical hydrophone arrays, and in processing, with advanced multiple removal and possibly depth filtering, is required to achieve the desired detailed images of the seismogenic plate boundary zone.     

DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants.

Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 micrograms/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development.