Population structure of Haliotis rubra from South Australia inferred from nuclear and mtDNA analyses

1Introduction ThemajorityofAustralia’sabalonefisheryex ports(5.135kt,worth＄216millionin2002～2003,ABARE2004)consistofblacklipabalone(HaliotisrubraLeach,1814).AssuchH.rubrais consideredasanimportantmarineresourcewithin Australia.Likemanyabalonespecieswor…     

化学物质对硬壳蛤幼虫变态的诱导

用KCl、肾上腺素（EPI）、去甲肾上腺素（NE）、L—DOPA和GABA（γ-氨基丁酸）进行了不同浓度不同处理时间对硬壳蛤（Mercenaria mercenaria L ）幼虫变态诱导实验。结果表明，KCl、肾上腺素、去甲肾上腺素和DDOPA对硬壳蛤幼虫的变态均有诱导作用，而GABA的诱导作用不显著。KCl的最佳诱导浓度随处理时间不同而有所不同。处理时间为1～24，48，72h时KCl的最佳诱导浓度分别为33．56，20．13～26．85，13．42mmol／L。肾上腺素和去甲肾上腺素的诱导作用与浓度和处理时间均有关。肾上腺素的最佳处理浓度为100μmol／L，最佳处理时间均为8h，此时幼虫变态率提高最大，为36．97个百分点。当去甲肾上腺素的诱导浓度为100μmol／L，处理时间为8～16h时，幼虫变态率提高也较大，均大于18个百分点，死亡率增加，但均低于30个百分点，当去甲肾上腺素诱导浓度为500μmol／L时，虽然在8～16h的处理时间范围内，幼虫变态提高率也较大，均大于18个百分点，但当处理时间超过8h，在16～48h范围内，幼虫死亡率提高明显增大，均大于50个百分点。L-DOPA的适宜诱导浓度为10～50μmol／L，适宜处理时间为8～24h，此时幼虫变态率均提高30个百分点以上，最高可提高79．43个百分点。GABA的诱导作用较弱，最佳诱导浓度随处理时间的不同而有所不同，处理时间为24h和48h时，最佳诱导浓度为0．1μmol／L；处理时间为0．5～16h时，最佳诱导浓度为100μmol／L。     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.     

湄洲湾泥沙活动及海底冲淤变化

湄洲湾沙源少,海水含沙量低,平均值10～20g/m~3。冬季受东北季风及浙闽沿岸流影响,湾口含沙量高,达到34g/m~3;夏季受径流影响,湾内含沙量相对偏高,达到16g/m~3。泥沙的运移趋势:主航道基本朝外,两侧朝里。受强潮流作用,深槽及水下潮流冲沟受到冲刷,斜坡及潮坪略有淤积。     

龙须菜在鱼藻混养系统中的生态功能

近年来 ,随着海水养殖产业的迅速发展 ,养殖规模的不断扩大 ,养殖区的自身污染问题也日益显露 ,以人工投饵和网箱养殖方式为主的浅海鱼类养殖 ,残饵和鱼体的代谢产物往往导致水体的富营养化和底质的有机污染 ,既影响周围环境 ,又不利于鱼类的生长和健康 ,长远意义上还会制约整个鱼类养殖业的持续发展。怎样减轻海水养殖造成的自身污染和对生态环境的破坏 ,已成为人们日益关注的问题 ,国内外学者已提出多种解决这一问题的途径 ,其中一条重要途径是通过优化养殖结构 ,实施综合养殖 ,发展生态养殖技术 (杨圣云等 ,1 996;杨红生 ,2 0 0 0 ;阎希…     

黄茅海河口湾现代动力地貌体系和冲淤过程分析

1980—1993年对黄茅海河口湾进行沉积物采样和水流测定及水深测量。根据水动力和地形条件，冲淤分析及Mclaren模型研究河口湾的动力地貌体系、冲淤特征和现代沉积物运移。结果表明：（1）水下地形主要为下泄流或上溯流控制的“深槽－槽沟－浅滩－湾口”的动力地貌体系，反映了河口湾“东进西出”的水流格局；（2）整个河口湾以淤积为主，只有崖门深槽有较明显的优势冲刷特征，并随着崖门深槽向海推移和河口湾“东进西出”水动力作用，黄茅海落潮三角洲相应向西南进积；（3）应用Mclaren模型揭示了黄茅海河口湾现代沉积物运移规律，同样反映了河口湾具有“东进西出”的运移趋势。     

海洋鱼类卵壳亚微结构的研究

在现代生物科学中,应用生物卵壳亚微结构的物种特异性进行分类研究已成为可行性现实。在海洋生物学研究中,曾发现海胆类未受精的卵壳层颗粒中即存在着眀显的物种特异性差别( L?nning,1967)。很多学者通过对鱼类卵细胞发生期间的亚微结构及卵壳形成的研究,证实在鱼类卵壳结构中亦存在特异性的种间差异,并认为这些差异与分类学、生物学及其区系分布有密切关系( Müller& Sterba,1963; Yamamoto,1963;G?tting,1966; Anderson,1967; Flügel,1967)。目前我国对鱼类卵壳( Egg chorin)结构的研究还鲜为人知,本文亦是首次通过对12种海洋经济鱼类受精卵的卵壳结构差别的探讨,为进行鱼类系统分类和系统发育研究提供科学依据。