Modeling rhamnolipids production by Pseudomonas aeruginosa from immiscible carbon source in a batch system

A mathematical model to predict the rhamnolipids production by Pseudomonas aeruginosa from oleic acid in a two phase liquid-liquid batch reaction system, was developed in this study. The model was based on two theoretical assumptions: 1) the convective oleic acid mass transfer is coupled to a bioreaction in the aqueous liquid bulk, and 2) the volume of the immiscible oleic acid drops and the saturation concentration at the interface are a function of rhamnolipids production. The model was able to accurately predict the experimental growth of the Pseudomonas aeruginosa strain, and the rhamnolipids production data with oleic acid as carbon source. This mathematical approach indicated a high correspondence between the saturation dimensionless profiles of oleic acid at the interface and the experimental profiles of surface tension difference. This modeling approach may constitute a useful tool in the design and scaling-up of bioreactors applied to the production of biosurfactants with immiscible carbon sources.     

Experimental constraints on ultrapotassic magmatism from the Bohemian Massif (durbachite series, Czech Republic)

The equilibrium phase relations of a mafic durbachite (53 wt.% SiO₂) from the Třebíč pluton, representative of the Variscan ultrapotassic magmatism of the Bohemian Massif (338–335 Ma), have been determined as a function of temperature (900–1,100°C), pressure (100–200 MPa), and H₂O activity (1.1–6.1 wt.% H₂O in the melt). Two oxygen fugacity ranges were investigated: close to the Ni–NiO (NNO) buffer and 2.6 log unit above NNO buffer (∆NNO + 2.6). At 1,100°C, olivine is the liquidus phase and co-crystallized with phlogopite and augite at 1,000°C for the whole range of investigated pressure and water content in the melt. At 900°C, the mineral assemblage consists of augite and phlogopite, whereas olivine is not stable. The stability field of both alkali feldspar and plagioclase is restricted to low pressure (100 MPa) at nearly water-saturated conditions (<3–4 wt.% H₂O) and T < 900°C. A comparison between experimental products and natural minerals indicates that mafic durbachites have a near-liquidus assemblage of olivine, augite, Ti-rich phlogopite, apatite and zircon, followed by alkali feldspar and plagioclase, similar to the mineral assemblage of minette magma. Natural amphibole, diopside and orthopyroxene were not reproduced experimentally and probably result from sub-solidus reactions, whereas biotite re-equilibrated at low temperature. The crystallization sequence olivine followed by phlogopite and augite reproduces the sequence inferred in many mica-lamprophyre rocks. The similar fractionation trends observed for durbachites and minettes indicate that mafic durbachites are probably the plutonic equivalents of minettes and that K- and Mg-rich magmas in the Bohemian Massif may have been generated from partial melting of a phlogopite–clinopyroxene-bearing metasomatized peridotite. Experimental melt compositions also suggest that felsic durbachites can be generated by simple fractionation of a more mafic parent and mixing with mantle-derived components at mid crustal pressures.     

Terrestrial photogrammetric techniques applied to the control of a parabolic dune in the Liencres dune system,Cantabria (Spain)

The spatial–temporal variations of a dune system can be determined by using diverse ‘geomatic’ methodologies: geodesy, global positioning system (GPS) and photogrammetry. In the case of the Liencres dune system, a study will be carried out using the ‘close‐range’ photogrammetry technique and the topography technique (total station and GPS). In order to determine the dynamic of the dune system it is necessary to repeat the process of study after a specific interval of time. For this reason, three dimensional data should be available in two different time periods, between which the displacement of the object of analysis (the front portion of the dune) will be significant enough to evaluate its magnitude. This work analyses the viability of photogrammetry for the determination of the spatial–temporal changes of a coastal parabolic dune. Two factors have been analysed: first, the comparison of the photogrammetric results with the results obtained from topographic methods (total station and GPS), and second, the quantification of the displacement of the dune system. The analysis of the correspondence between the movement of different parts of the dune and the influence of the intensity and direction of the prevailing wind in the area is also desired. The dune advanced 12·15 ± 0·06 m (an average of 8·5 m/yr), and the partial implications for the dynamic of human modified processes on the natural park have been established. Copyright © 2008 John Wiley & Sons, Ltd.     

PULSE: a finite element code for solving adiabatic nonradial pulsation equations

We briefly present the nonradial adiabatic pulsation code PULSE first developped for white dwarf asteroseismology and now used to compute adiabatic oscillation properties for various types of stellar objects. Numerical tests show that the code is able to provide the accuracy (for a given stellar model) required to deal with the precision in frequency expected from the COROT long runs. While the ultimate objective is to compare the output of various pulsation codes (see these proceedings), we already emphasize problems that need to be addressed concerning, in particular, the mesh resolution of the input stellar models and its impact on the accuracy at which frequencies can be computed.     

Magnetic susceptibility and electrical conductivity as a proxy for evaluating soil contaminated with arsenic,cadmium and lead in a metallurgical area in the San Luis Potosi State,Mexico

A total of 113 samples of waste and soil were collected from a site in the state of San Luis Potosi, Mexico, that was occupied for several years by the metallurgical industry. Specific magnetic susceptibility (MS), electrical conductivity (EC) and pH were determined, as well as the total and available concentrations of potentially toxic elements (PTEs) such as As, Cd, Cu, Fe, Pb and Zn, which may cause a health risk for humans, animals and ecosystems, and the concentrations of major ions in aqueous extracts of soils and wastes. The solid phases of the samples were also characterized. The results revealed that the soils and wastes exhibited elevated values of PTEs, MS and EC. For soils these values decreased with increasing distance from the waste storage sites. The MS values were elevated primarily due to the presence of Fe-oxyhydroxides, such as magnetite, hematite and goethite, which contain PTEs in their structure leading to a high correlation between the value of MS and the As, Cd, Fe and Pb contents (r = 0.57–0.91) as well as between the PTEs values (r = 0.68–0.92). The elevated EC values measured in the metallurgical wastes were the result of presence of the sulfate minerals of Ca, Mg and Fe. The pollution index, which indicates the levels of simultaneous toxicity from elements such as As, Cd and Pb, was determined, with extreme hazard zones corresponding to areas that exhibit high MS values (0.91 correlation). In conclusion, MS measurements can be used as an indirect indicator to evaluate the PTE contamination in metallurgical areas, and EC measurements can aid in the identification of pollution sources.     

Persistent organic pollutants and histological lesions in Mayan catfish Ariopsis assimilis from the Bay of Chetumal, Mexico

Livers of catfish (Ariopsis assimilis) from the Bay of Chetumal were analyzed for organochlorine compounds and hydrocarbons as part of a study to diagnose the environmental health of the Bay after a catfish mass mortality that occurred in 1996. The presence of histological lesions in several organs of the fish as result of chemical exposure was also evaluated. The concentrations of organic pollutants found in the Bay may be considered high if compared to the levels reported for sites affected by chemical pollution. High prevalences of cellular alteration histopathologies were found in liver, including hepatic tumors. The presence of some lesions may be related statistically to environmental pollution in the Bay, specially with chlorinated compounds.     

Rockfall Hazard Analysis From Discrete Fracture Network Modelling with Finite Persistence Discontinuities

Developing an accurate representation of the rock mass fabric is a key element in rock fall hazard analysis. The orientation, persistence and density of fractures control the volume and shape of unstable blocks or compartments. In this study, the discrete fracture modelling technique and digital photogrammetry were used to accurately depict the fabric. A volume distribution of unstable blocks was derived combining polyhedral modelling and kinematic analyses. For each block size, probabilities of failure and probabilities of propagation were calculated. A complete energy distribution was obtained by considering, for each block size, its occurrence in the rock mass, its probability of falling, its probability to reach a given location, and the resulting distribution of energies at each location. This distribution was then used with an energy–frequency diagram to assess the hazard.     

The microwave structure of coronal condensations and its relation to proton flares

Active regions on the Sun in the 20th solar cycle are studied with special reference to their association with proton flares based on microwave interferometric observations at Toyokawa Observatory. It has been reconfirmed that the active regions associated with intense S-component emission with a high 3-cm to 8-cm flux ratio are likely to produce proton flares. About one fourth of 259 active regions during the period investigated are found to have definite features in the spatial distribution of polarization at a wavelength of 3 cm. Active regions with one particular type of polarization pattern have a good correlation with the occurrence of proton flares.     

Helical structures around quiescent solar prominences computed from observable magnetic fields

We analyse the magnetic support of solar prominences in two-dimensional linear force-free fields. A line current is added to model a helical configuration, well suited to trap dense plasma in its bottom part. The prominence is modeled as a vertical mass-loaded current sheet in equilibrium between gravity and magnetic forces.We use a finite difference numerical technique which incorporates both vertical photospheric and horizontal prominence magnetic field measurements. The solution of this mixed boundary problem generally presents singularities at both the bottom and top of the model prominence. The removal of the singularities is achieved by superposition of solutions. Together with the line current equilibrium, these three conditions determine the amplitude of the magnetic field in the prominence, the flux below the prominence and the current intensity, for a given height of the line current. A numerical check of accuracy in the removal of singularities, is done by using known analytical solutions in the potential limit.We have investigated both bipolar and quadrupolar photospheric regions. In this mixed boundary problem the polarity of the field component orthogonal to the prominence is mainly fixed by the imposed height of the line current. For bipolar regions above (respectively below) a critical height the configuration is inverse (respectively normal). For quadrupolar regions the polarity is reversed if we refer the prominence polarity to the closest photospheric polarities. We introduce the polarity of the component parallel to the prominence axis with reference to a sheared arcade. Increasing the shear with fixed boundary conditions can increase or decrease the mass supported depending on the configuration.