Adenine oxidation by pyrite-generated hydroxyl radicals

Cellular exposure to particulate matter with concomitant formation of reactive oxygen species (ROS) and oxidization of biomolecules may lead to negative health outcomes. Evaluating the particle-induced formation of ROS and the oxidation products from reaction of ROS with biomolecules is useful for gaining a mechanistic understanding of particle-induced oxidative stress. Aqueous suspensions of pyrite particles have been shown to form hydroxyl radicals and degrade nucleic acids. Reactions between pyrite-induced hydroxyl radicals and nucleic acid bases, however, remain to be determined. Here, we compared the oxidation of adenine by Fenton-generated (i.e., ferrous iron and hydrogen peroxide) hydroxyl radicals to adenine oxidation by hydroxyl radicals generated in pyrite aqueous suspensions. Results show that adenine oxidizes in the presence of pyrite (without the addition of hydrogen peroxide) and that the rate of oxidation is dependent on the pyrite loading. Adenine oxidation was prevented by addition of either catalase or ethanol to the pyrite/adenine suspensions, which implies that hydrogen peroxide and hydroxyl radicals are causing the adenine oxidation. The adenine oxidation products, 8-oxoadenine and 2-hydroxyadenine, were the same whether hydroxyl radicals were generated by Fenton or pyrite-initiated reactions. Although nucleic acid bases are unlikely to be directly exposed to pyrite particles, the formation of ROS in the vicinity of cells may lead to oxidative stress.     

Apatite as an indicator mineral for mineral exploration: trace-element compositions and their relationship to host rock type

Over 700 apatite grains from a range of rock types have been analysed by laser-ablation microprobe ICPMS for 28 trace elements, to investigate the potential usefulness of apatite as an indicator mineral in mineral exploration. Apatites derived from different rock types have distinctive absolute and relative abundances of many trace elements (including rare-earth elements (REE), Sr, Y, Mn, Th), and chondrite-normalised trace-element patterns. The slope of chondrite-normalised REE patterns varies systematically from ultramafic through mafic/intermediate to highly fractionated granitoid rock types. (Ce/Yb)_cn is very high in apatites from carbonatites and mantle-derived lherzolites (over 100 and over 200, respectively), while (Ce/Yb)_cn values in apatites from granitic pegmatites are generally less than 1, reflecting both HREE enrichment and LREE depletion. Within a large suite of apatites from granitoid rocks, chemical composition is closely related to both the degree of fractionation and the oxidation state of the magma, two important parameters in determining the mineral potential of the magmatic system. Apatite can accept high levels of transition and chalcophile elements and As, making it feasible to recognise apatite associated with specific types of mineralisation. Multivariate statistical analysis has provided a user-friendly scheme to distinguish apatites from different rock types, based on contents of Sr, Y, Mn and total REE, the degree of LREE enrichment and the size of the Eu anomaly. The scheme can be used for the recognition of apatites from specific rock types or styles of mineralisation, so that the provenance of apatite grains in heavy mineral concentrates can be determined and used in geochemical exploration.     

Uranium content and radiogenic ages of hypersthene,bronzite, amphoterite and carbonaceous chondrites

U was measured by fission track analysis in 115 samples of hypersthene, bronzite, amphoterite and carbonaceous chondrites. On a weight basis the average values for the Cl carbonaceous and bronzite chondrites are similar to the “classic” value of 11 ppb, but the hypersthenes and amphoterites are ~50 per cent higher. Each class shows a well-determined peak in the U abundance distribution, allowing the calculation of radiogenic ages and comparison with other elements of interest.     

Timing of magma extraction during the Campanian Ignimbrite eruption (Campi Flegrei Caldera)

A core drilled within the northern part of the city of Napoli has offered the unique opportunity to observe in one single sequence the superposition of the four pyroclastic flow units emplaced during the Campanian Ignimbrite (CI) eruption. Such a stratigraphic succession has never been encountered before in natural or in man made exposures. Therefore the CI sequence was reconstructed only on the basis of stratigraphic correlations and compositional data (in literature). The occurrence of four superposed CI flows, together with all the data available (in literature) allowed us to better constrain the chemical stratigraphy of the deposit and the compositional structure of the CI magma chamber. The CI magma chamber includes two cogenetic magma layers, separated by a compositional gap. The upper magma layer was contaminated by interaction with radiogenic fluids. The two magma layers were extruded either individually or simultaneously during the course of the eruption. In the latter case they produced a hybrid magma. But no evidence of input of new geochemically and isotopically distinct magma batches just prior or during the eruption has been found. Comparison with the exposed CI deposits has permitted reconstruction of variable eruption phases and related magma withdrawal and caldera collapse episodes. The eruption was likely to have began with phreatomagmatic explosions followed by the formation of a sustained plinian eruption column fed by the simultaneous extraction from both magma layers. Towards the end of this phase the upward migration of the fragmentation surface and the decrease in magma eruption rate and/or activation of fractures formed an unstable pulsating column that was fed only by the most-evolved magma layer. This plinian phase was followed by the collapse of the eruption column and the beginning of caldera formation. At this stage expanded pyroclastic flows fed by the upper magma layer in the chamber generated. During the following major caldera collapse episode, the maximum mass discharge rate was reached and both magma layers were tapped, generating expanded pyroclastic flows. Towards the end of the eruption, only the deeper and less differentiated magma layer was tapped producing more concentrated pyroclastic flows that traveled short distances.     

Carbon-14 production compared to oxygen isotope records from Camp Century,Greenland and Devon Island,Canada

Carbon-14 production rate variations that are not explainable by geomagnetic changes are thought to be in antiphase with solar activity and as such should be in antiphase with paleotemperature records or proxy temperature histories such as those obtainable from oxygen isotope analyses of ice cores. Oxygen isotope records from Camp Century, Greenland and Devon Island Ice Cap are in phase with each other over thousands of years and in antiphase to the ¹⁴C production rate residuals.     

Coronagraphic observations of an enhanced coronal region: I,Fexii and Nixv emission line data

Nine coronal emission lines representing five stages of Fe ionization and one stage of Ni were observed in an enhanced coronal region. The data from these observations are presented along with a density model of the enhanced region obtained from the FeXIII and NiXV emission line ratios as a function of position angle. The electron densities obtained from FeXIII lines range from N _e = 10⁸ to 10⁹ cm^–3, and are slightly lower for NiXV line data. Estimates of the variation of temperature over the enhanced region are inferred from the observed line intensities.     

Possible effect of subsurface inhomogeneities on the lunar microwave spectrum

Inhomogeneities beneath the lunar surface could alter the average microwave emission spectrum of the Moon in a fashion generally consistent with observations, even in the absence of an average heat flux or density gradients with depth. The lunar subsurface was modeled as an inhomogeneous lossy dielectric with three-dimensional refractive index fluctuations characterized by independent horizontal and vertical correlation lengths. The model suggests that attempts to infer the physical properties of the Moon from the lunar microwave spectrum could be significantly inaccurate if subsurface scattering were neglected.     

Single- and two-phase fluid flow properties of cataclastic fault rocks in porous sandstone

Understanding the impact of faults on fluid flow in the subsurface is important for the extraction of oil, gas and groundwater as well as the geological storage of waste products. We address two problems present in current industry-standard workflows for fault seal analysis that may lead to fault rocks not being represented adequately in computational fluid flow models. Firstly, fluid flow properties of fault rocks are often measured only for small-scale faults with throws not exceeding a few centimetres. Large seismic-scale faults (throws >20 m) are likely to act as baffles or conduits to flow but they are seldom recovered from subsurface cores and consequently fault rock data for them is sparse. Secondly, experimental two-phase fluid flow data is lacking for fault rocks and, consequently, uncertainties exist when modelling flow across faults in the presence of two or more immiscible phases. We present a data set encompassing both single- and two-phase fluid flow properties of fault and host rocks from the 90-Fathom fault and its damage zone at Cullercoats Bay, NE England. Measurements were made on low-throw single and zones of deformation bands as well as on slip-surface cataclasites present along the ～120 m throw main fault. Samples were analysed using SEM and X-ray tomography prior to petrophysical measurements. We show that single deformation bands, deformation band zones and slip-surface cataclasites exhibit dissimilar single- and two-phase fluid flow properties. This is due to grain-size reduction being more pronounced in slip-surface cataclasites and changes in microstructure being fault-parallel for deformation bands but mostly fault-perpendicular for slip-surface cataclasites. A trend of fault rocks with low absolute permeabilities exhibiting lower relative permeabilities than more permeable rocks at the same capillary pressure is evident.