Effect of stocking density on water quality and (Growth,Body Composition and Plasma Cortisol Content) performance of pen-reared rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>)

The goal of the study was to examine the effect of stocking density on the water quality of culture area, as well as the growth, body composition and cortisol content of rainbow trout（Oncorhynchus mykiss）. Pen-reared trout were stocked in densities of 40, 60, 80 fish individuals m^-3(4.6, 6.6, 8.6 kg m^-3, SD1, SD2 and SD3 groups, respectively) for 300 days. Compared to the water from SD1 and SD2, that from SD3 exhibited significantly higher NH₄⁺-N content and COD（chemical-oxygen-demand）, and a significant reduction of dissolved oxygen in day 180(40.6 kg m^-3). Stocking density was significantly associated with body weight, standard length, VSI（viscerosomatic index）, CF（condition factor） and FC（food coefficient） in group SD3, particularly in day 240 and day 300(45 or 49.3 kg m^-3). Increased crude fat and decreased crude protein were displayed in high density group when the density reached to 36 kg m^-3. As a cumulative effect of density-related stress, VSI, CF, FC, moisture, and crude protein content varied over time in each density group（SD1, SD2, and SD3）. In summary, trout exhibited a better growth performance in low density(26.3 kg m^-3) than those reared in high densities(36 and 45 kg m^-3). The results indicate that rainbow trout（114.44 g ± 6.21 g, 19.69 cm ± 0.31 cm） initially stocked in 6.6 or 8.6 kg m^-3 should be lightened to less than 36 kg m^-3 after an intensive rearing for 240 days.     

Effect of benzo[a]pyrene on detoxification and the activity of antioxidant enzymes of marine microalgae

The objective of this study was to examine the effect of benzo[a]pyrene(Ba P) on the detoxification and antioxidant systems of two microalgae,Isochrysis zhanjiangensis and Platymonas subcordiformis.In our study,these two algae were exposed to Ba P for 4 days at three different concentrations including 0.5 μg~(L-1)(low),3 μg~(L-1)(mid) and 18 μg~(L-1)(high).The activity of detoxification enzymes,ethoxyresorufin O-deethylase(EROD) and glutathione S-transferase(GST) increased in P.subcordiformis in all Ba P-treated groups.In I.zhanjiangensis,the activity of these two enzymes increased at the beginning of exposure,and then decreased in the groups treated with mid-and high Ba P.The activity of antioxidant enzyme superoxide dismutase(SOD) increased in I.zhanjiangensis in all Ba P-treated groups,and then decreased in high Ba P-treated group,while no significant change was observed in P.subcordiformis.The activity of antioxidant enzyme catalase(CAT) increased in I.zhanjiangensis and P.subcordiformis in all Ba Ptreated groups.The content of malondialdehyde(MDA) in Isochrysis zhanjiangensis increased first,and then decreased in high Ba P-treated group,while no change occurred in P.subcordiformis.These results demonstrated that Ba P significantly influenced the activity of detoxifying and antioxidant enzymes in microalgae.The metabolic related enzymes(EROD,GST and CAT) may serve as sensitive biomarkers of measuring the contamination level of Ba P in marine water.     

Numerical study on inter-tidal transports in coastal seas

Inter-tidal(subtidal) transport processes in coastal sea depend on the residual motion, turbulent dispersion and relevant sources/sinks. In Feng et al.(2008), an updated Lagrangian inter-tidal transport equation, as well as new concept of Lagrangian in- ter-tidal concentration(LIC), has been proposed for a general nonlinear shallow water system. In the present study, the LIC is nu- merically applied for the first time to passive tracers in idealized settings and salinity in the Bohai Sea, China. Circulation and tracer motion in the three idealized model seas with different topography or coastline, termed as ‘flat-bottom', ‘stairs' and ‘cape' case, re- spectively, are simulated. The dependence of the LIC on initial tidal phase suggests that the nonlinearities in the stairs and cape cases are stronger than that in the flat-bottom case. Therefore, the ‘flat-bottom' case still meets the convectively weakly nonlinear condi- tion. For the Bohai Sea, the simulation results show that most parts of it still meet the weakly nonlinear condition. However, the de- pendence of the LIS(Lagrangian inter-tidal salinity) on initial tidal phase is significant around the southern headland of the Liaodong Peninsula and near the mouth of the Yellow River. The nonlinearity in the former region is mainly related to the complicated coast- lines, and that in the latter region is due to the presence of the estuarine salinity front.     

Growth performance of larval and juvenile manila clam (<Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>) from divergently selected individuals of a full-sib family

In this study, the method of divergent selection was employed to test the larval and juvenile growth performance within a full-sib family of Manila clam Ruditapes philippinarum. The 10% largest and 10% smallest clam individuals (on the basis of shell length) of a full-sib family were selected as parents for the fast and slow growing lines, respectively. The difference in shell length was significant among the three lines (fast, control, and slow) tested. The sequence of shell length were fast line > control line > slow line. The responses to selection, realized heritability, and genetic gain were 0.06%–0.81%, 0.04%–0.47% and 0.58%–18.89% in the fast direction, respectively; and were 0.14%–1.27%, 0.08%–0.73%, and 0.31%–49.03% in the slow direction, respectively. The results suggested that there was a large portion of additive genetic variance affecting the growth in the full-sib family. Selection in the fast direction within the full-sib family would greatly improve the growth of R. philippinarum.     

Characteristics of groundwater flow field after land creation engineering in the hilly and gully area of the Loess Plateau

In order to study the degree of influence and control mechanism to groundwater flow field caused by land creation engineering in the hilly and gully area of the Loess Plateau, based on the geological and engineering conditions of the first stage project of Yan’an new district in China, numerical simulation of groundwater flow is carried out by the Feflow and GIS technologies. From the simulation, punning measure relatively reduces infiltration recharge and artificial gravel drain increases groundwater seepage. The basic characteristics of groundwater flow field is controlled by the old and new topographies in the whole study area, and artificial gravel drain plays an auxiliary role in accelerating groundwater drawdown upstream and promotes groundwater rise downstream. According to differences of groundwater level and declining percentages of hydraulic gradient in the main and secondary gullies, dewatering of artificial gravel drain in the secondary gully is more effective than that in the main gully, which will yet play an important role in the future. The study results will make contributions to understand groundwater response to land creation engineering and will be beneficial to take necessary measures to prevent collapse of loess and failure of building foundation in the hilly and gully area of the Loess Plateau.     

基于无人船的大洋中尺度涡观测系统展望

中尺度涡在大洋中普遍存在,研究发现其能量比大尺度海洋环流的能量大一个量级,在海洋物质能量输运和全球气候变化中起着重要的作用。受观测条件限制,目前对中尺度涡的观测主要通过卫星高度计实现,只能从海面高度来推算中尺度涡大小、分布、强度及其伴随的水体和能量输送,而卫星高度计对中尺度涡垂直结构特征认识不足,也导致了对中尺度涡所引起的上层海洋能量、热量输送估计误差偏大。目前对中尺度涡三维结构观测认识不足,展望未来将会出现基于无人船平台的大洋中尺度涡三维结构自动观测系统,该平台将集成自动水下剖面观测功能等先进技术,以便观测中尺度涡的垂直结构特征及其时空变化特征,进而可对中尺度涡带来的物质和能量输送进行系统认识。