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51.
52.
山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析 总被引:26,自引:7,他引:26
采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场,各20尾,取全血或肌肉组织,以酚-仿抽提方法提取基因组DNA,利用筛选获得的10对微卫星引物进行PCR扩增,反应产物经8%非变性聚丙烯酰胺凝胶电泳分离、EB显色,用ImageMasterl D Elite(Version 3.01)软件分析电泳结果,并计算了相应的遗传学参数。结果表明,在自然和养殖群体中,10个基因座位的等位基因平均数(α)分别为6.7和6.1,每个基因座位有效等位基因数(αe)分别为1.8—6.8和2.5—6.7,群体平均杂合度(H)分别为0.8120和0.7310;两个群体间的遗传相似性系数、遗传距离和基因分化系数为0.8558、0.1557和0.0558;自然群体内每个座位上的多态信息含量(PIC)为0.59—0.84、个体识别率(DP)为0.54—0.86、非父排除率(PPE)为0.41—0.72,其累积个体识别率和非父排除率均达到0.9999,表明所选座位属中高识别力的遗传标记,可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。 相似文献
53.
中太平洋铁锰结壳铅同位素研究 总被引:5,自引:0,他引:5
已有研究表明大洋中溶解的铅(Pb)来源于陆源物质,但是,对Pb进入大洋的途径争议很大。为此分析了取自中太平洋两块铁锰结壳样品的Pb同位素组成,获得了整个新生代的中太平洋Pb同位素演化历史。结果表明这两块结壳的Pb同位素随时间的演化曲线与中北太平洋沉积物岩心LL44-GPC3中风成碎屑的Pb同位素演化曲线相似。证实该区深水中的天然溶解铅主要来自风成粉尘,并且50Ma之前中太平洋中溶解Pb同位素组成主要取决于源自美洲的风成粉尘的输入,40Ma之后主要取决于源自亚洲的风成粉尘的输入。 相似文献
54.
An offshore vessel with a dynamic positioning system (DP system) needs fast response to produce thrust to counteract the environmental forces acting on it for the purpose of maintaining its position and heading as close as possible to the working position. Therefore, quick and effective modulation of the thrust is the problem to determine the thrust and the rotation angle of the thruster devices of the ship. This paper presents an effective optimum control for a thruster system, using the sequential quadratic method to achieve economical and effective modulation of the thrust and the direction of the thruster. An optimum control study of a 2280 tons DP coring vessel with five rotary azimuth thruster marine positioning is studied in detail, which can quickly and exactly estimate the thrusts and angles of direction of all the thrusters. The results can provide a valuable thruster system for a dynamically positioned vessel. 相似文献
55.
A new set of equations of motion for wave propagation in water with varying depth is derived in this study. The equations expressed by the velocity potentials and the wave surface elevations include first-order non-linearity of waves and have the same dispersion characteristic to the extended Boussinesq equations. Compared to the extended Boussinesq equations, the equations have only two unknown scalars and do not contain spatial derivatives with an order higher than 2. The wave equations are solved by a finite element method. Fourth-order predictor–corrector method is applied in the time integration and a damping layer is applied at the open boundary for absorbing the outgoing waves. The model is applied to several examples of wave propagation in variable water depth. The computational results are compared with experimental data and other numerical results available in literature. The comparison demonstrates that the new form of the equations is capable of calculating wave transformation from relative deep water to shallow water. 相似文献
56.
This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr. 相似文献
57.
58.
On low-pass digital filters in oceanography 总被引:1,自引:0,他引:1
-Two types of filters are widely used to remove semidirunal and diurnal tidal signals and other high frequency noises in oceanography. The first type of filters uses moving average with weights in time domain, and can be easily operated. Some data will be lost at each end of the time series, especially for the low low-pass filters. The second type of filters uses the discrete Fourier transform filter (DFTF) which operates in the frequency domain, and there are no data loss at the ends for the forward transform. However, owing to the Gibbs phenomenon and the discrete sampling (Nyquist effect) , ringing appears in the inverse transformed data, which is especially serious at each end. Thus some data at the ends are also discarded. The present study tries to find out what causes the ringing and then to seek for methods to overcome the ringing. We have found that there are two kinds of ringings, one is the Gibbs phenomenon, as defined before. The other is the "Nyquist"ringing due to sampling Nyquist critical 相似文献
59.
基于拉格朗日余流及其输运过程的一种三维空间弱非线性理论,进一步假定Si在河口的无因次化学转移项量级为k^2,给出了Si的长期输运方程。对于一种二维模型河口,数值求解了零阶天文潮,欧拉余流、斯托克斯漂移、拉格朗日余流和盐度的分布;分别计算了平均逼留时间为13,30,60,180d等所对应的Si浓度分布;给出了Si-s相关图,讨论了河口中Si的保守性问题。 相似文献
60.