HPLC法同步检测鲤鱼、对虾中喹乙醇与MQCA残留

建立了一种高效液相色谱法同时检测鲤鱼、对虾肌肉组织中喹乙醇（OLA）及其代谢物3-甲基-喹啉-2-羧酸（MQCA）的残留。采用0.3 mol/L 盐酸提取鲤鱼、对虾中的残留物, C18 固相萃取柱净化, 氮气吹干及流动相定容后, Waters2695 高效液相色谱仪在320 nm（MQCA）和372 nm（QLA）双波...     

对北祁连山火山喷溢型贱金属硫化物矿床区域成矿的探讨

北祁连山加里东优地槽褶皱山系由厚层海相火山岩系和沉积建造组成。其内已经发现数十个火山岩型块状硫化物矿床,可划分为三种类型,即Cu(Fe)型(或红沟型)、Cu-Zn型(或蛇绿岩套型)及Cu-Pb-Zn型(或白艰厂型)。不同类型矿床分别形成于不同地质环境且趋向于在特定时-空范围产出:Cu(Fe)型矿床集中分布在南部达坂山成矿带,成矿与晚奥陶世双峰态细碧角斑岩系有关,矿床形成于弧间或弧后盆地环境;Cu-Zn型矿床分布在北部九个泉—错沟、猪嘴哑吧一银硐沟矿带,与早—中奥陶世蛇绿岩套有关,矿床形成于大洋扩张构造环境;Cu-Pb-Zn型矿床集中分布在祁连、白银成矿区,矿床产出与中寒武世中酸性石英角斑凝灰岩有关,形成于优地槽发展早期阶段裂谷岛弧环境。     

挪威某些铜镍矿石中单斜磁黄铁矿出溶体的退火和粗化

在挪威某些正岩浆铜镍矿床的矿石中，六万磁黄铁矿基质内的单斜磁黄铁矿叶片是高温时形成的磁黄铁矿固溶体在降温过程中出溶的产物。出溶叶片宽度和间距的加大、叶片的带状分布、楔形尖端、箱状扭曲和复杂叶片等结构，表明出溶产物在不高于单斜-六方转变温度（254℃）的条件下发生过显著的退火和粗化。重结晶的六方磁黄铁矿基质中所保存的单斜变种甚少，表明变质作用所引起的矿石重结晶，可使磁黄铁矿吸收其中的单斜出溶体而发生均匀化。     

Imposex and surface sediment speciation: a combined approach to evaluate organotin contamination in Guanabara Bay, Rio de Janeiro, Brazil

An integrated biological-chemical survey of organotin compounds was carried out in Guanabara Bay, the second most important Brazilian harbor complex. The biological survey revealed high levels of imposex in Stramonita haemastoma populations. Inside the bay values of relative penis length index from 42.7 to 107.6 and vas deferens sequence index from III to VI were found, while organisms collected outside the bay had values ranging from <0.1 to 35.2 and from 0 to II. None of the females sampled inside the bay were normal and imposex was found in all stations. Surface sediments in the bay are contaminated by tributyltin (10-522 ng/g d.w.) and triphenyltin (<3.9-39.4 ng/g d.w.) with greater concentrations close to shipyards and marinas. The observed predominance of parent compounds (TBT and TPT) is commonly found when recent inputs occur, but may also indicate slow degradation processes in the anoxic conditions of these sediments. To our knowledge, this is the first report of a combined imposex-sediment approach to evaluate organotin contamination in marine environments of South America, and also the first report of TPT detection in environmental matrices in this region.     

An easy, non-destructive, probabilistic method to evaluate the imposex response of gastropod populations

Endocrine disruption in marine populations is a growing concern. One of the best documented examples is imposex, the development of male characteristics in female gastropods, including the development of a penis and/or vas deferens, by exposure to organotins, mainly tributyltin. This syndrome has been an effective and widely used biomarker of organic tin contamination for almost 20 years. However, established imposex evaluation protocols sacrifice all the animals and repeated assessments can result in depletion of populations. To preserve animal lives as much as possible and to have a fast screening of areas subjected to organotin impacts, a non-destructive approach was tested. This approach uses reversible narcotization and a statistical criterion to evaluate the extent of imposex in the populations, instead of imposex indexes, which are based on evaluation of sacrificed, fixed animals. The data consist of two categories: males plus masculinized females in one category and normal females in another. The probability is then calculated that this sample could have been drawn from a normal population. This new method was tested in two contrasting areas of Brazil and with two different species, and the results compared to the conventional evaluation. Narcotization was completely reversed with a survival rate of 98%. Impacted sites could be identified with this technique in the same way as with the conventional one. The only limitation was that female sterility could not be verified. This disadvantage could well be compensated by the minimal mortality in the studied populations.     

海洋节肢动物细胞及组织培养研究进展

细胞培养是生物工程中重要手段和常用方法之一,已广泛应用于染色体分析、细胞代谢、基因表达、免疫、癌变机制、病毒培养、分离、鉴定和药物筛选等研究领域。作为生物学领域中的一项重要技术,动物组织和细胞培养已广泛应用于哺乳动物和鱼类的研究中,并取得了丰硕成果。但由于海洋节肢动物的