海带中细胞激动素的纯化分离方法

海带样品于 2 0 0 1年采自青岛太平角海湾 ,用三种不同方法处理样品 ,通过对实验方法及条件的筛选 ,确立了提取、纯化和分离海带中细胞激动素的方法步骤和流程。结果表明 ,最佳方法的基本步骤为 :( 1 )用甲醇∶水∶醋酸 ( 70∶ 30∶ 3,V/V/V)从海带组织中提取出细胞激动素 ,再用PVPP柱去除提取液中的酚类和其他杂质 ;( 2 )将提取液通过连在一起的PVPP柱、DEAE柱和C1 8柱 ,以纯化样品并同时从自由基、核苷、核苷酸和葡糖苷型细胞激动素混合物中分离出核苷酸型细胞激动素 ;( 3)用接有氨基柱的正相液相色谱进一步将葡糖苷型细胞激动素分离出来 ;( 4 )用C1 8液相色谱柱分离其他各种细胞激动素     

A fuzzy approach to the lectotype optimization of offshore platforms

Lectotype optimization of offshore platforms is of particular importance in the concept design process. Lectotype optimization involves multiple objectives with uncertainty and so is a problem of multiple attribute decision making. To date, there have been few published works on this topic in the context of offshore engineering. This paper develops a framework and methodology for evaluation of offshore platform alternatives, where a fuzzy optimum model is proposed to integrate the influence of each objective in the criteria set, and a new weight-assessing method is developed to mimic the decision maker’s experience and preference based on complementary pairwise comparisons. A case study shows that the new framework and methodology is scientific, reasonable and easy to use in practice.     

生物胺对锯缘青蟹大颚器发育的调节作用

雌性锯缘青蟹(Scylla serrata)于实验的第1天、第5天、第10天，分别注射100μL剂量为1μg／g体质量的5-HT，OA和DA，第15天解剖。结果表明，5-HT对锯缘青蟹大颚器发育具有显的促进作用，OA和DA的作用不明显。经免疫细胞化学检测，大颚器内存在5-HT免疫阳性细胞，免疫阳性细胞多数为卵圆形，少数具有很长的胞突，该结果首次为5-HT参与大颚器的生理活动提供了形态学证据。     

A Unified Variational Principle of Fluid Mechanics and Application on Solitary Subdomain or Point

-According to basic equations of fluid mechanics, this paper presents a unified variational principle of fluid mechanics (UVPFM) by using the optimization method of weighted residuals (OMWR). The advantages are as follows, the establishment of the functional and the variational principle is easy, it can change various problems of fluid mechanics derived by basic equations into a unified optimization problem, and the solution is the optimum one in some sense. According to the OMWR for the solitary subdomain, this paper uses UVPFM onto any solitary subdomain and gives the solution of the hydrodynamics equation which is suitable only for that solitary subdomain. According to the OMWR for solitary point, this paper uses UVPFM to any solitary point and gives the solution of the hydrodynamics equation (point solution) which is suitable only for that solitary point. As the solution for the solitary subdomain or solitary point is developed independently, the compatibility with other subdomain or other points, do     

Prediction of Load Carrying Capacity of Corroded Reinforced Concrete Beam

A novel method for prediction of the load carrying capacity of a corroded reinforced concrete beam (CRCB) is presented in the paper. Nine reinforced concrete beams, which had been working in an aggressive environment for more than 10 years, were tested in the laboratory. Comprehensive tests, including flexural test, strength test for corroded concrete and rusty rebar, and pullout test for bond strength between concrete and rebar, were condueted. The flexural test results of CRCBs reveal that the distribution of surface cracks on the beams shows a fractal behavior. The relationship between the fractal dimensions and mechanical properties of CRCBs is then studied. A prediction model based on artificial neural network (ANN) is established by the use of the fractal dimension as the corrosion index, together with the basic intbrmation of the beam. The validity of the prediction model is demonstrated through the experimental data, and satisfactory resuits are achieved.     

南沙群岛海区冬夏季中、深层海流的计算

根据１９８８年夏季和１９８９年冬季南沙群岛海区两个航次的温、盐度调查资料，运用β螺旋方法对调查海区的海流进行了计算。计算结果表明，该海区表、中层环流相对比较一致，深层流动状态与表、中层的差异较大，其中的局地环流的季节变化不明显。此外，在北康暗沙与南薇滩之间，夏季中、深层和冬季深层都明显反映出存在着一个迄今未见报道的局地反气旋式环流。文章对上述结果与该海区已有的一些海流分析结果作了比较。     

台湾海峡海流结构及海水通量

主要根据台湾海峡的实测海流资料，以夏、冬为代表季节，分析了台湾海峡２—３个纬向断面的海流结构，计算出各断面的海水通量。结果表明：夏季，台湾海峡中、北部海域各层的海流一般偏Ｎ向流动，Ｎ向的海水净通量为３．３２×１０６ｍ３·ｓ－１；冬季，高温高盐的黑潮水和南海水由南向北经南部断面进入台湾海峡，其海水通量分别为１．６９×１０６ｍ３·ｓ－１和０．５９×１０６ｍ３·ｓ－１；而东海水由北向南通过北部断面进入台湾海峡，其海水通量为１．０２×１０６ｍ３·ｓ－１，其中，有０．４０×１０６ｍ３·ｓ－１的海水沿着福建和广东近岸流进南海，其余０．６２×１０６ｍ３·ｓ－１的海水在台湾海峡北部混合后随同黑潮水和南海水流入东海。总之，流经台湾海峡的Ｎ向海水净通量为１．７４×１０６ｍ３·ｓ－１。     

吡喹酮和克螨特复配驱虫制剂对一些水生饵料生物的毒性

由吡喹酮和克螨特组成的复配制剂对小球藻、轮虫、蚤状溞、水蚯蚓和卤虫的毒性实验结果显示：复配制剂对轮虫的半致死浓度(LC50)为13．9mg／dm^3(24h)和3．03mg／dm^3(48h)．安全浓度(SC)为0．0432mg／dm^3；对蚤状溞的LC50为345．14mg／dm^3(24h)和17．5mg／dm^3(48h)，SC为0．0135mg／dm^3；对水蚯蚓的LC50为636．80mg／dm^3(24h)和79．07mg／dm^3(48h)，SC为0．366mg／dm^3；对卤虫无节幼虫的LC50为201．84mg／dm^3(24h)和8．91mg／dm^3(48h)．SC为0．00519mg／dm^3,复配制剂对水环境生物的破坏作用小．     

北黄海长山群岛海域沉积环境初步研究

本文在获取１９８９－１９９３年黄海北部长山群岛海域沉积物大量数据基础上，借助沉积物粒度分析（Ｑ型），揭示出该海域具有在“岛屿效应”影响下的４种环境能量类型，分析了与其适应的环岛沉积环境及其分布特征。     

Influence of Kuroshio water on the distribution of dissolved oxygen in the northern East China Sea

On the basis of the four-cruise investigation data of the Kuroshio by the R/V "Xiangyanghong 09", this paper lays stress on the discussion of the relationship between the Kuroshio current systems, the distribution of dissolved oxygen in the northern East China Sea (ECS) and seasonal variations.