Land-use-mediated <Emphasis Type="Italic">Escherichia coli</Emphasis> concentrations in a contemporary Appalachian watershed

A high-spatial resolution study design was used to investigate the relationship between land use practices, stream physicochemistry, hydroclimate, and stream Escherichia (E) coli concentrations in a mixed-land-use watershed in the Appalachian region. Stream samples were collected daily from six monitoring sites and analyzed for total E. coli counts using an enzyme metabolism indicator method. Statistical comparison of E. coli concentration time series showed significant (p?<?0.05) differences between study sites. Although highest average E. coli concentrations were observed at two agricultural sites (534 and 582 colony-forming counts (CFU) per 100 mL, respectively), highest total loadings were observed within the receiving stream, with values increasing downstream (2?×?10¹² and 4.2?×?10¹² study total CFU for bracketed upstream and downstream sites, respectively). No single physical variable displayed a significant correlation (p?<?0.05) with observed E. coli concentration at every site. However, sites displayed different patterns of significant correlations (p?<?0.05) between E. coli concentration and both physicochemical (e.g. pH, dissolved oxygen saturation) and hydroclimate variables (e.g. streamflow and precipitation). Percent agricultural land cover was the only land use category that showed significant (p?<?0.04) correlation with study average E. coli concentrations, thereby emphasizing the importance of land use practices to stream pathogen regimes. Results validate the analytical method and provide high-resolution, detailed, quantitative characterizations of stream E. coli regimes, thereby supplying land and water resource managers with science-based information to advance management decisions and improve public health.     

Adaptation behavior in the face of global climate change: Survey responses from experts and decision makers serving the Florida Keys

We conduct a survey to elicit responses from experts and decision makers serving the Florida Keys regarding vulnerability to global climate change. Study findings reveal deep concern among federal, state and local experts and decision makers about adverse impacts at the local level. A large majority of respondents recognize the increasing likelihood of dynamic, potentially irreversible, socioeconomic and ecological repercussions for the Florida Keys. However, very few experts and decision makers report that their respective agencies have developed formal adaptation plans. Respondents identify significant institutional and social barriers to adaptation and convey their support for a host of strategic measures to facilitate adaptation on an urgent basis. The implications of our findings are discussed in the context of enhancing adaptive capacity and resilience in the Florida Keys and beyond. Information generated from this study can provide functional guidance for improving decision-support systems and promoting adaptation policies.     

Stochastic analysis of flux and head moments in a heterogeneous aquifer system

This study investigates the behavior of flux and head in a strongly heterogeneous three-dimensional aquifer system. The analyses relied on data from 520 slug tests together with 38,000 one-foot core intervals lithological data from the site of the General Separations Area in central Savannah River Site, South Carolina, USA. The skewness in the hydraulic conductivity histograms supported the geologic information for the top two aquifers, but revealed stronger clay content, than was reported for the bottom aquifer. The log-normal distribution model described adequately the hydraulic conductivity measurements for all three aquifers although, other distributions described equally well the bottom aquifer measurements. No apparent anisotropy on the horizontal plane was found for the three aquifers, but ratios of horizontal to vertical correlation lengths between 33 and 75 indicated a strong stratification at the site. Three-dimensional Monte Carlo stochastic simulations utilized a grid with larger elements than the support volume of measurements, but of sub-REV (representative elementary volume) dimensions. This necessitated, on one hand, the use of upscaled hydraulic conductivity expressions, but on the other hand did not allow for the use of anisotropic effective hydraulic conductivity expressions (Sarris and Paleologos in J Stoch Environ Res Risk Assess 18: 188–197, 2004). Flux mean and standard deviations components were evaluated on three vertical cross-sections. The mean and variance of the horizontal flux component normal to a no-flow boundary tended to zero at approximately two to three integral scales from that boundary. Close to a prescribed head boundary both the mean and variance of the horizontal flux component normal to the boundary increased from a stable value attained at a distance of about five integral scales from that boundary. The velocity field 〈q_x〉 was found to be mildly anisotropic in the top two aquifers, becoming highly anisotropic in the bottom aquifer; 〈q_y〉 was anisotropic in all three aquifers with directions of high continuity normal to those of the 〈q_x〉 field; finally, 〈q_z〉 was highly anisotropic in all three aquifers, with higher continuity along the east–west direction. The mean head field was found to be continuous, despite the high heterogeneity of the underlying hydraulic conductivity field. Directions of high continuity were in alignment with field boundaries and mean flow direction. Conditioning did not influence significantly the expected value of the flux terms, with more pronounced being the effect on the standard deviation of the flux vector components. Conditioning reduced the standard deviations of the horizontal flux components by as much as 50% in the bottom aquifer. Variability in the head cross-sections was affected only marginally, with an average 10% reduction in the respective standard deviation. Finally, the location of the conditioning data did not appear to have a significant effect on the surrounding area, with uniform reduction in standard deviations.     

Sea-level change and paleogeographic reconstructions,southern Vancouver Island,British Columbia,Canada

Forty-eight new and previously published radiocarbon ages constrain deglacial and postglacial sea levels on southern Vancouver Island, British Columbia. Sea level fell rapidly from its high stand of about +75 m elevation just before 14 000 cal BP (12 000 radiocarbon yrs BP) to below the present shoreline by 13 200 cal BP (11 400 radiocarbon years BP). The sea fell below its present level 1000 years later in the central Strait of Georgia and 2000 years later in the northern Strait of Georgia, reflecting regional differences in ice sheet retreat and downwasting. Direct observations only constrain the low stand to be below ?11 m and above ?40 m. Analysis of the crustal isostatic depression with equations utilizing exponential decay functions appropriate to the Cascadia subduction zone, however, places the low stand at ?30 ± 5 m at about 11 200 cal BP (9800 BP). The inferred low stand for southern Vancouver Island, when compared to the sea-level curve previously derived for the central Strait of Georgia to the northwest, generates differential isostatic depression that is consistent with the expected crustal response between the two regions. Morphologic and sub-bottom features previously interpreted to indicate a low stand of ?50 to ?65 m are re-evaluated and found to be consistent with a low stand of ?30 ± 5 m. Submarine banks in eastern Juan de Fuca Strait were emergent at the time of the low stand, but marine passages persisted between southern Vancouver Island and the mainland. The crustal uplift presently occurring in response to the Late Pleistocene collapse of the southwestern sector of the Cordilleran Ice Sheet amounts to about 0.1 mm/yr. The small glacial isostatic adjustment rate is a consequence of low-viscosity mantle in this tectonically active region.     

Optical spectra of supernova remnant candidates in the Sculptor Group galaxy NGC 300

We present moderate-resolution (<5 Å) long-slit optical spectra of 51 nebular objects in the nearby Sculptor Group galaxy NGC 300 obtained with the 2.3 meter Advanced Technology Telescope at Siding Spring Observatory, Australia. Adopting the criterion of [S?ii]_Total:Hα≥0.4 to confirm supernova remnants (SNRs) from optical spectra, we find that of 28 objects previously proposed as SNRs from optical observations, 22 meet this criterion with six showing [S?ii]_Total:Hα of less than 0.4. Of 27 objects suggested as SNRs from radio data, four are associated with the 28 previously proposed SNRs. Of these four, three (included in the 22 above) meet the criterion. In all, 22 of the 51 nebular objects meet the [S ii]_Total:Hα criterion as SNRs while the nature of the remaining 29 objects remains undetermined by these observations.     

Opening of the red sea with two poles of rotation

Previous studies have shown that the Red Sea was formed by two stages of sea-floor spreading, with a quiescent period in between. We suggest that these two phases have occurred in different directions. The shape of the central trough indicates that the present-day motion is almost E-W, whereas the total opening, deduced from the shape of the coastlines, is NE-SW. If the axial trough has opened in an E-W direction, the earlier stage of opening was in a direction which made the Dead Sea Rift fall along a small circle to the pole of early opening, and hence suggests that the Dead Sea Rift was a transform fault during this early stage. The later movement gives almost pure extension along the Dead Sea Rift, and this should be seen by normal faulting. Available first-motion studies are not precise enough to confirm or deny this hypothesis.     

Vegetation controls on channel stability in the Bell River,Eastern Cape,South Africa

Channel instability has occurred in the Bell River in the form of meander cutoffs, a number of which have occurred since 1952. Increased sediment loading from widespread gully erosion in the catchment has been proposed as the trigger for this instability. Willow species of the Salix family, in particular S. caprea, have been planted along the banks in an effort to prevent further channel shifting. This study reports the results of an investigation into the effect of vegetation on channel form and stability over a 17 km stretch of channel. Results indicate that riparian vegetation has significant effects on channel form which have implications for channel stability. Riparian vegetation increases bank stability and reduces channel cross-sectional area, thereby inducing stability at flows less than bankfull. Evidence indicates that narrow stable stretches are associated with relatively high levels of riparian vegetation. Wider, unstable channels are associated with relatively less riparian vegetation. The effectiveness of riparian vegetation relative to bank sediments was investigated. A dense growth of willows was found to have an equivalent effect to banks with a silt-clay ratio of about 70 per cent. The channel narrowing induced by vegetation may contribute to channel shifting at high flows. The reduced channel capacity is thought to result in more frequent overbank flooding which may ultimately lead to channel avulsion. Thus where increased sediment loading is pushing the channel towards instability, vegetation may be effective in imparting local stability, but it is unable to prevent long-term channel shifts, and may rather help to push the system towards more frequent avulsions. Copyright © 1999 John Wiley & Sons, Ltd.     

Conjugation of 4-hydroxynonenal by largemouth bass (Micropterus salmoides) glutathione S-transferases

The glutathione S-transferases (GST) are a major group of conjugative enzymes involved in the detoxification of electrophilic compounds and products of oxidative stress. We have previously described the kinetics of hepatic GST conjugation in largemouth bass using a variety of synthetic GST reference substrates. In the present study, we investigated the ability of largemouth bass hepatic GSTs to conjugate 4-hydroxynon-2-enal (4HNE), a mutagenic and cytotoxic alpha-beta-unsaturated aldehyde produced during oxidative injury. Hepatic cytosolic fractions from largemouth bass rapidly catalyzed GSH-dependent 4HNE conjugation, with the rate of GST-4HNE conjugation in bass liver exceeding those of several other mammalian and aquatic species. No apparent sex-related differences in GST-4HNE activity were observed among adult bass. SDS-PAGE and Western blotting analysis of GSH affinity-purified bass liver cytosolic GST revealed the presence of two major GST subunits of approximately 30 and 27 KDa that exhibited slight cross-reactivity when probed with a rat alpha class GST antibody, but not to rat mu, pi or theta class GST. The rapid conjugation of 4HNE by hepatic GST suggests an important role for GSTs in protecting against peroxidation of polyunsaturated fatty acids in bass liver.