Experimental study on prevention of acid mine drainage by silica coating of pyrite waste rocks with amorphous silica solution

Acid mine drainage (AMD) is a widespread environmental problem associated with working and abandoned mining operations. It results from the microbial oxidation of pyrite in the presence of water and air, affording an acidic solution that contains toxic metal ions. Pyrite microencapsulation, utilizing silica coating, is a novel approach for controlling AMD that has been shown to be very effective in controlling pyrite oxidation. The roles of the solution pH and silica concentration in the formation mechanism for the AMD-preventing coating were investigated. A silica coating can be formed from silica solution at pH 7, at which the amount of Fe eluted from pyrite into the solution is small. No coating was formed at other pH values, and the amounts of eluted Fe were larger than at pH 7, especially at pH 11. The silica coating forms from 2,500 to 5,000 mg/L silica solutions, but not from 0 or 1,000 mg/L silica solutions. The coating formation rate was slower in the 2,500 mg/L silica solution than in the 5,000 mg/L silica solution. The formation of silica coating on pyrite surfaces depends on three main steps: formation of Fe(OH)₃ on the surface of pyrite, reaction between Fe(OH)₃ and silicate in the solution on the pyrite surface, and growth of the silica layer on the first layer of silica. The best pH condition to enable these steps was around 7, and the silica coating formation rate can be controlled by the concentration of silica.     

Model analysis of radar echo split observed in an artificial cloud seeding experiment

An artificial cloud seeding experiment was performed over the Japan Sea in winter to show how massive seeding could be effective to mitigate heavy snowfall damage. The results showed that 20 min after cloud seeding, a portion of the radar echo beneath the seeding track was weakened to divide the radar echo into two parts. In order to analyze the results, a numerical simulation was conducted by using the Weather Research and Forecasting model verion 3.5.1. In this simulation, the seeding effects were represented as phenomena capable of changing rain particles by accreting cloud ice and snow to form graupel particles and by changing cloud liquid water to snow particles. The graupel particles fell rapidly, thus temporarily intensifying the rainfall, which subsequently decreased. Therefore, the weakened radar echo in the field experiment is deemed to have been caused by the increase in rapidly falling graupel particles.     

The inventory and chemical characterization of dissolved proteins in oceanic waters

One-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) were applied to separate protein molecules in dissolved organic matter (DOM) from oceanic waters. Results were: (1) The 2-DE distinguished a total of 412 protein spots in 10 samples from five water columns over the Pacific, although fewer than 30 proteins were resolved as bands from the identical samples by SDS-PAGE. (2) Major and ubiquitous protein bands (34 and 39 kDa proteins) on the SDS-PAGE gel were resolved into horizontally spread arrays (trains) of spots on the 2-DE gels, indicating that these bands were a mixture of protein species that have the same molecular weight (MW) but different isoelectric points (pIs). (3) Proteins that exhibited such electrophoretic patterns on the 2-DE gels were glycosylated with variable linkages between the sugar and polypeptide chains. (4) N-terminal amino-acid sequencing demonstrated that individual spots within each train of spots had identical N-terminal amino-acid sequences.The N-terminal amino-acid sequences of the 39 and 34 kDa glycoprotein spots in samples collected at different sites were also identical. Protein isoforms with the same amino-acid sequence but different glycosylation profiles, termed glycoforms, were often observed on the 2-DE gel. Thirty-one and 24 spots on the 2-DE gels were glycoforms of two glycoproteins with MWs of 39 and 34 kDa, respectively; they were one protein species. The glycoforms of the 39 kDa protein were identified as a low molecular weight alkaline phosphatase (L-AP) of Pseudomonas aeruginosa PAO1 by a homology search through five amino-acid sequence databases. The present and earlier work indicates that all identified source organisms of dissolved proteins belong to the Pseudomonas group. We propose the hypothesis that proteins associated with membrane vesicles liberated from a minor member of the bacterioplankton assemblage, the marine Pseudomonas group, are one of the important sources of dissolved proteins in oceanic waters. This hypothesis may apply to the source pathway and survival not only of proteins and also to the universally occurring bacterial peptidoglycan and lipopolysaccharide components in DOM.     

Vertical transport of organic materials in the northern North Pacific as determined by sediment trap experiment

Sediment traps were deployed at 5 depths of 100 through 5,250 m to collect suspended sediments in the northern North Pacific (47°51.1'N; 176°20.6'E, 5,300 m deep) in the summer of 1978. Fatty acid composition was determined in the samples of phytoplankton, particulate matter, trap sediment and bottom sediment.Fatty acid composition of the trap sediments revealed no significant vertical trend throughout the water column from depths of 100 to 5,250 m, and were also similar to those of the phytoplankton and the particulate matter from the euphotic layer. However, a marked difference in the fatty acid composition was observed between the trap sediments and the particulate matter from deep waters. Therefore, it can be concluded that the source of fatty acids in the trap sediments is the particulate matter from the euphotic layer but not from deep waters.Unsaturated fatty acids highly susceptible to biological agents were rather abundant in the trap sediments as well as in the phytoplankton and particulate matter from the euphotic layer, however no unsaturated fatty acid was found in the particulate matter from deep waters. From these findings, it is clear that the particulate matter of the euphotic layer is transported to deep waters very rapidly. As the sinking rate of fecal pellets produced by zooplankton is in the range of ten to hundreds of meters a day, fecal pellets are assumed to be the most likely carrier of rapid-transport of organic matter including fatty acids from the euphotic layer to deep waters.     

Stability and P–V–T equation of state of KAlSi3O8-hollandite determined by in situ X-ray observations and implications for dynamics of subducted continental crust material

In situ X-ray diffraction measurements of KAlSi₃O₈-hollandite (K-hollandite) were performed at pressures of 15–27 GPa and temperatures of 300–1,800 K using a Kawai-type apparatus. Unit-cell volumes obtained at various pressure and temperature conditions in a series of measurements were fitted to the high-temperature Birch-Murnaghan equation of state and a complete set of thermoelastic parameters was obtained with an assumed K′_300,0=4. The determined parameters are V _300,0=237.6(2) Å³, K _300,0=183(3) GPa, (?K _T,0/?T) _P =?0.033(2) GPa K^?1, a ₀=3.32(5)×10^?5 K^?1, and b ₀=1.09(1)×10^?8 K^?2, where a ₀ and b ₀ are coefficients describing the zero-pressure thermal expansion: α _T,0 = a ₀ + b ₀ T. We observed broadening and splitting of diffraction peaks of K-hollandite at pressures of 20–23 GPa and temperatures of 300–1,000 K. We attribute this to the phase transitions from hollandite to hollandite II that is an unquenchable high-pressure phase recently found. We determined the phase boundary to be P (GPa)=16.6 + 0.007 T (K). Using the equation of state parameters of K-hollandite determined in the present study, we calculated a density profile of a hypothetical continental crust (HCC), which consists only of K-hollandite, majorite garnet, and stishovite with 1:1:1 ratio in volume. Density of HCC is higher than the surrounding mantle by about 0.2 g cm^?3 in the mantle transition zone while this relation is reversed below 660-km depth and HCC becomes less dense than the surrounding mantle by about 0.15 g cm^?3 in the uppermost lower mantle. Thus the 660-km seismic discontinuity can be a barrier to prevent the transportation of subducted continental crust materials to the lower mantle and the subducted continental crust may reside at the bottom of the mantle transition zone.     

Distribution of fossil seawater and its role in Neogene sedimentary rock landslides in Niigata,eastern marginal region of the Japan Sea

The Neogene marine sedimentary rock area in the eastern marginal region of the Japan Sea is an area with some of the highest landslide densities in Japan. Some of the landslides in this area have been known to involve saline groundwater, which can be the cause of these landslides. In order to demonstrate the relationships between landslides and saline water, topographic, geological, groundwater, and electromagnetic surveys were performed in the eastern marginal region of the Japan Sea. Many landslides and gravitational slope deformations with linear depressions and small scarps were recognized in the study area. The resistivity profile obtained by an electromagnetic survey suggests that there is a wide zonal distribution of saline water with salt concentrations equivalent to seawater at depths of 50–100 m or more and that the groundwater shallower than 50 m has an electrical conductivity of less than 100 mS/m. The shallow resistive groundwater is inferred to be meteoric water that replaced the saline groundwater, which likely weakened the bedrock, resulting in landslides. A ridge of competent tuff overlying mudstone has many linear depressions from gravitational slope deformation and low‐resistivity water to a depth of 600 m, which suggests that the mudstone was weakened by water replacement and deformed under the tuff caprock. The saline groundwater is inferred to be fossil seawater trapped in pores during sediment deposition, which is brought near the ground surface along with rocks by tectonic movement in the hills. Thus, the saline water and its fresh water replacement are among the important basic causes of the landslides. The oil well data obtained in the eastern marginal region of the Japan Sea suggest that such saline water replacement has occurred widely and that replacement is likely one of the predispositions for the frequent landslides there.     

Probing molecular and isotopic features of meteoritic insoluble organic matter by solid‐state nuclear magnetic resonance spectroscopy with sub‐milligram sample

We recorded one‐ and two‐dimensional solid‐state nuclear magnetic resonance spectra of meteoric insoluble organic matter with unprecedented sensitivity and resolution permitting us to reveal unambiguous spectroscopic fingerprints relevant to its molecular and isotopic features. Two‐dimensional ¹H‐¹H and ¹H‐¹³C correlation experiments have unveiled the spatial proximity between aliphatic and aromatic groups proving a highly branched character of a rigid macromolecular network composed of short aliphatic chains linking together small aromatic units. One‐dimensional ²H and two‐dimensional ¹H‐²H correlation spectroscopy delivered evidence of significant reduction in the deuterium enrichment of aromatic species relevant to interstellar processes, proto‐planetary disk chemistry, and to determining the origin of the meteoritic insoluble organic matter. The experimental approach developed in this work opens new perspectives for systematic and nondestructive analysis at the molecular level of meteoritic insoluble organic matter even with a very small amount of sample from some particularly rare chondrites.     

Field-based description of near-surface crustal deformation in a high-strain shear zone: A case study in southern Kyushu,Japan

This study investigated geological evidence for near-surface crustal deformation in a high-strain shear zone that has been geodetically identified but which is not associated with obvious tectonic landforms. Fieldwork was conducted in the east–west-trending southern Kyushu high-strain shear zone (SKHZ), Japan, focusing mainly on occurrences of fracture zones, which are defined by a visible fracture density of >1 per 10 cm² and are commonly associated with cataclasite, fault breccia, and gouge. The area in which east–west-trending fracture zones are dominant is restricted to the east–west-trending, ~2-km-wide aftershock area of the 1997 Northwestern Kagoshima Earthquakes. Analysis of slip data from minor faults using the multiple inverse method, irrespective of whether the faults are in fracture zones, reveals that the area where the calculated main stress field is consistent with the current stress field estimated from focal-mechanism solutions of microearthquakes is restricted to the east–west-trending aftershock area. This finding for the SKHZ contrasts with the case of the Niigata–Kobe Tectonic Zone, which is a major strain-concentration zone with many exposed active faults in central Japan and for which the stress field estimated using fault-slip data is considered to be uniform and coincides with the current stress field. The cumulative amount of displacement estimated from the areal density of fracture zones in the SKHZ study area is smaller than that estimated from geodetically measured strain rates. Investigations based on slip data from minor faults and fracture-zone occurrence could help to identify concealed faults that are too small to generate tectonic landforms but which are sufficiently large to trigger major earthquakes.     

Determination of the phase boundary between ilmenite and perovskite in MgSiO3 by in situ X-ray diffraction and quench experiments

Determination of the phase boundary between ilmenite and perovskite structures in MgSiO₃ has been made at pressures between 18 and 24 GPa and temperatures up to 2000 °C by in situ X-ray diffraction measurements using synchrotron radiation and quench experiments. It was difficult to precisely define the phase boundary by the present in situ X-ray observations, because the grain growth of ilmenite hindered the estimation of relative abundances of these phases. Moreover, the slow reaction kinetics between these two phases made it difficult to determine the phase boundary by changing pressure and temperature conditions during in situ X-ray diffraction measurements. Nevertheless, the phase boundary was well constrained by quench method with a pressure calibration based on the spinel-postspinel boundary of Mg₂SiO₄ determined by in situ X-ray experiments. This yielded the ilmenite-perovskite phase boundary of P (GPa) = 25.0 (±0.2) – 0.003 T (°C) for a temperature range of 1200–1800 °C, which is generally consistent with the results of the present in situ X-ray diffraction measurements within the uncertainty of ∼±0.5 GPa. The phase boundary thus determined between ilmenite and perovskite phases in MgSiO₃ is slightly (∼0.5 GPa) lower than that of the spinel-postspinel transformation in Mg₂SiO₄. Received: 19 May 1999 / Accepted: 21 March 2000