Mechanisms of uranium mineralization by the yeast Saccharomyces cerevisiae

We determined the association of uranium in yeast cells S. cerevisiae grown in medium containing high (1 g · L^-1) or low (0.2 g · L^-1) concentrations of phosphate after exposure for 96 h to a 4 × 10^-4 mol · L^-1 U(VI) solution at pH 3.2 or 4.7. The analysis was made using a field emission scanning electron microscope equipped with energy dispersive spectroscopy (FESEM-EDS), transmission electron microscopy (TEM), and visible diffuse reflectance spectrometry. Cells grown in the high-phosphate medium rapidly accumulated U(VI) from solution at pH 3.2 over the first 24 h, followed by a slow uptake until 96 h, whereas in cells grown in low-phosphate medium, U(VI) accumulation reached a steady state within 24 h. FESEM-EDS analyses revealed the formation of a U(VI)-bearing precipitate on the yeast cells grown in high-phosphate medium after only 48 h exposure; no precipitate was detected on cells grown in low-phosphate medium up to 96 h. These results suggest that sorption onto the cell surfaces was the dominant process initially. Analysis of the U(VI)-bearing precipitates by all three methods demonstrated the presence of H-autunite, HUO₂PO₄ · 4H₂O. Thermodynamic calculations suggest that the chemical compositions of the solutions containing yeast grown in high-phosphate medium were undersaturated with respect to H-autunite, but were supersaturated with ten times more U(VI) and P than were actually observed. Apparently, the sorbed U(VI) on the cell surfaces reacts with P released from the yeast to form H-autunite by local saturation. The U(VI) uptake by yeast cells grown in high phosphate medium at pH 4.7, along with the thermodynamic calculation, indicated that more H-autunite is precipitated in neutral pH solution than in acid solution. Thus, U(VI)-phosphate mineralization on the cells of microorganisms should be taken into account for predicting U(VI) mobility in the environment.     

Neogene geological history of the Tohoku Island Arc system

The Pacific-type orogeny in the Tohoku Island Arc is discussed using marine geological and geophysical data from both Pacific and Japan Sea along the Tohoku region. The Tohoku Arc is divided into three belts; inner volcanic and sedimentary belt, intermediate uplifted belt and outer sedimentary trench belt. Thick Neogene sediments which are distinguished in several layers by continuous seismic reflection profiling occur on both sides of the intermediate belt. The dominant structural trend of the Neogene layers is approximately parallel to the coast line and to the axis of the Japan Trench and has a extension of approximately 100 km in each unit on the Pacific side. The trench slope break is an uplifted zone of Neogene layers. The structural trend of the upper continental slope and outer shelf is relative uplift of the landward side. Tilted block movement toward the west is the dominant structural trend on the Japan Sea side. Structural trends which can be seen in both the inner and outer belts may suggest horizontal compressional stress of east to west. Orogenesis and tectogenesis in the Tohoku Arc has been active since early Miocene or latest Oligocene. It may be implied that the Japan Trench was not present during Late Cretaceous to Paleogene, as is suggested by the volcanism of the Tohoku Arc. The basic framework of the present structure was formed during late Miocene to early Pliocene in both the inner and outer belts. Structural movements were reactivated during late Pleistocene.     

Primary migration theory of petroleum and its application to petroleum exploration

Current concepts of oil and gas generation by thermal decomposition of kerogen are reviewed. Primary oil migration mechanisms requiring large quantities of water to serve as a carrier for the movement of oil from source rock to reservoir are discussed. Previous investigators regarded the expulsion of interlayer water from montmorillonite and montmorillonite-illite mixed-layer minerals by transformation during diagenesis as the most important source of the carrier water.The process of diagenesis is subdivided into three stages based on the results of experimental compaction studies on montmorillonite clay, on studies of the expulsion mechanism of interlayer and interstitial waters, and on the observed changes in pores and mineral grains in argillaceous sediments during these stages. As a result, we concluded that the migration of oil chiefly occurs during the late compaction stage when the sediment's porosity ranges from 30 to 10%.This conclusion implies that a large amount of oil can move during the period when the active generation of oil corresponds with the primary migration of carrier water. For instance, in the oil-bearing Miocene sediments of the Akita area of northern Japan, the oil generation temperature ranged from 100 to 150°C and the average migration depth was between 1300 and 2600 m. Therefore, the possibility of large oil pool formation will be high in the basin where the paleo-geothermal gradient is about 5.0°C/100 m. In fact, it is expected that the paleo-geothermal gradients in the vicinities of large oil fields will be very close to this value.Prediction of the type and amount of hydrocarbons in the exploration area will be possible by an examination of the paleo-geothermal gradient in the area and by a study of the relation between absolute porosity and burial depth of argillaceous rocks.     

Occurrence of PSP-producing dinoflagellate Alexandrium tamiyavanichii in Bingo-Nada, the central coastal water of the Seto Inland Sea, Hiroshima Prefecture, Japan

During surveillance of the distribution of the paralytic shellfish poison (PSP)-producing dinoflagellate in 2003, 2004 and 2005 along the coastlines of the Seto Inland Sea, Hiroshima Prefecture, Japan, some species of toxic phytoplankton were isolated from the eastern coasts, Bingo-Nada, the central regions of the Seto Inland Sea. It was rather unexpectedly revealed from the basis of the morphological characteristics that they were unambiguously identified as Alexandrium tamiyavanichii and Alexandrium catenella. Two strains (ATY041106, ATY051018) of A. tamiyavanichii showed a specific toxicity of 38.7 x 10(-6) and 111.5 x 10(-6)MU/cell, respectively. These values seemed to be several times or much higher than that of A. catenella (AC030816, AC040614), having a specific toxicity of 4.5 x 10(-6) and 4.1 x 10(-6)MU/cell, respectively, isolated in the same area. From the results of HPLC-furuorometric analysis, it revealed that the toxins in ATY041106 exist almost exclusively as beta-epimers (C2, GTX3, GTX4), which accounted for 72.7 mol%. The toxin profiles of this strain are featured by the presence of a large amount of GTX3 (59.1 mol%) and a small amount (20.6%) of C1 and 2 in comparison with the PSP compositions of A. tamarense, which is isolated as the main responsible species in Hiroshima Bay, a western part of coastal sea in Hiroshima Prefecture. On the other hand, it revealed that the toxin profiles of two strains (AC030816, AC040614) of A. catenella exist almost exclusively as beta-epimers (C2, GTX3, GTX4), which accounted for 81.8 and 56.5 mol%, as the same manner. The toxin profiles of these two strains are featured by the presence of a large amount of C2 (80.5 and 46.3 mol%) in comparison with the PSP compositions of A. tamiyavanichii. To our knowledge, this is the first record to show the distribution and harmful influence of A. tamiyavanichii and A. catenella in Bingo-Nada in Hiroshima Prefecture. Though contamination of bivalves with these PSP-producing planktons in this area has not occurred yet so far, attention should be paid to this species as well as the other causative dinoflagellate from the stand point of public health and food hygiene.     

Subduction erosion and accretion in the Solomon Sea region

The Solomon Sea region is an area of intense tectonic activity characterized by structural complexity, a high level of seismicity and volcanism, and rapid evolution of plate boundaries. There is little accretion in the eastern New Britain Trench. Accretion gradually increases westward with thick accretion in the western New Britain Trench and in the Trobriand Subduction System. The thick accretion in the western part of the New Britain Trench may be a result of collision from the north of Finisterre-Huon block with New Guinea mainland. The present boundary of the collision is along the Ram-Markham fault. Deformation structures and present day seismicity suggest that the northern block is under compression.

Accretion has occurred in the sediment filled trenches in the Solomon Sea. The scale of the accretionary wedge depends on the amount of trench-fill sediment available. It is unlikely that there is no sediment supply to the eastern part of the New Britain Trench where no accretion is observed and subduction erosion may be occurring. There are two possible mechanisms for subduction erosion of sediment; either a rapid rate of subduction relative to the supply of sediment inhibiting sediment accumulation in the trench; or horizontal tensional force superimposed on both the forearc and backarc regions of the arc. Seafloor spreading in both the Manus and Woodlark basins is fan-like with nearby poles in the western margins of the basins. This may be a reflection of a horizontally compressional field in the western part and a tensional field in the eastern part of the Solomon Sea. Therefore it is possible to conclude that the consumption of sediment in the eastern New Britain Trench is related to the horizontal tensional field superimposed on both the forearc and backarc regions of the subduction system.

Imbricated thrust and overthrust faults in the western New Britain Trench and Trobriand Trough are not linear over long distance, but form wavy patterns in blocks with unit distance of approximately 10 km.     

Diurnal variation of CO2 concentration, Δ14C and δ13C in an urban forest: estimate of the anthropogenic and biogenic CO2 contributions

Diurnal variation in the atmospheric CO₂ concentration and the carbon isotopic composition (Δ¹⁴C and δ¹³C) was measured in a forest in an urban area on 9 February 1999. The carbon isotope approach used in the present study differentiated between the quantitative contributions from anthropogenic and biogenic CO₂ sources in the urban atmosphere. The anthropogenic (fossil fuel) and biogenic (soil respiration) contributions was estimated, and they ranged from 1 to 16% and from 2 to 8% of the total atmospheric CO₂. The diurnal variation of the anthropogenic CO₂ was the major cause of the total atmospheric CO₂ variation, while the biogenic CO₂ remained relatively constant throughout the day. Estimating the contribution of soil respired CO₂ provided the mean residence time of soil respired CO₂ within the forest atmosphere.     

Effects of flow dimension in faulted or fractured rock on natural reductions of inflow during excavation: a case study of the Horonobe Underground Research Laboratory site,Japan

Major inflows of groundwater can occur during excavation in faulted or fractured rock masses, even if pre-excavation grouting is applied; postexcavation grouting may then be required to reduce these inflows. However, the diffusion equation for fluid pressure suggests that inflows may reduce naturally by 50–90% or more within days or weeks when the dimension of the flow in faults or fractures that feed the inflow (flow dimension) is close to 1, but inflow reduction is minimal when the flow dimension is close to 3. Therefore, if the flow dimension is close to 1, the natural reduction in inflow may obviate countermeasures. Nevertheless, this natural reduction being dependent on flow dimension is seldom considered explicitly when planning excavations or countermeasures. To verify the applicability of the relationship between natural changes of inflow and the flow dimension, this study measured changes in inflow at six locations at the Horonobe site, Japan, where major inflows occurred during excavations of tunnels or shafts in faulted or fractured siliceous rocks. The flow dimension at each location was assessed using pre-excavation packer tests in surface-based boreholes. The results confirm that changes in the inflows during the days and weeks immediately after their commencement depended consistently on the assessed flow dimension. Natural reductions in inflow during excavation are predictable based on the flow dimension, which can be estimated using pre-excavation borehole investigations or from the initial changes in inflow during the first several days. This approach may be helpful for improving the efficiency of excavations.

     

Carbon cycle and climate change during the Cretaceous inferred from a biogeochemical carbon cycle model

The carbon cycle and climate change during the Cretaceous are reconstructed by using a carbon cycle model, and discussed. The model takes into account the effects of the enhanced magma eruption and organic carbon burial rates, both of which characterize the carbon cycle during the Cretaceous. The result for the CO₂ variation is roughly consistent with the pattern of paleoclimate change inferred from the geological record. The CO₂ level during the mid-Cretaceous is estimated to be 4–5 times the present atmospheric level, corresponding to a surface temperature of 20–21°C. The warm, equable Cretaceous resulted from the effects of tectonic forcing such as enhanced CO₂ degassing, although the enhanced organic carbon burial has a tendency to decrease the CO₂ level. The organic carbon burial rate during the Cretaceous is generally larger than those for the Cenozoic, and is characterized by three major peaks (~ 1.5–1.8 times the present-day value) corresponding to the major oceanic anoxic events. In the case for the extensive mantle plume degassing, although the CO₂ levels are only 10% higher than those for the standard case during 120–100 Ma, the causes for the enhanced CO₂ levels would be quite different. If the globally averaged surface temperature had increased due to paleogeographic forcing effects, the greenhouse effect of CO₂ (and thus the CO₂ level) should be lower than the values estimated for the standard case. If the CO₂ levels are similar to, but the surface temperature is higher than, those for the standard case, either the parameter β (an influence of the Himalayas–Tibetan Plateau on the global weathering today) may be unreasonably large or the dependence of the silicate weathering rate on the CO₂ partial pressure and the surface temperature should be much weaker than those previously proposed.     

Age estimation of the Solomon Sea based on heat flow data

Several heat flow measurements were made during the NAT83 cruise in the central part of the Solomon Sea Basin. The average value of 87 mW/m² (2.08 HFU) calculated from these and other data indicates that the age of the Solomon Sea Basin may range from 24 to 44 Ma. This is supported by the water depth, of approximately 4,500 m, versus age relationship. There is a possibility that the Solomon Sea Basin is not a back-arc basin associated with an arc but was formerly a relatively large oceanic plate. The agreement in age from both heat flow and water depth data favors the latter hypothesis.