藻类分子生物技术两年评─—与藻类活性物质研究有关的生物技术

根据１９９２年６月－１９９４年６月国内外发表资料，评述海洋藻类活性物质研究的生物技术及最新成果。认为，海洋生物活性物质研究的重心正转向与海洋生物技术有关的研究。运用生物技术筛选、提取并生产藻类活性物质已经成为这一领域的主流。综合文献表明，藻类活性物质的生物技术正走向成熟，大量的活性物质被发现，光生物反应器将成为主要的下游生产技术，通过藻类基因工程生产药物已初现端倪。     

白洋淀重新蓄水后的轮虫

1989年对白洋淀重新蓄水后的轮虫作了采样调查,共采到轮虫123种,隶属于46属12科,并对鉴定到种的轮虫作了初步分析。     

Effects of variable winds on biological productivity on continental shelves in coastal upwelling systems

The production and distribution of biological material in wind-driven coastal upwelling systems are of global importance, yet they remain poorly understood. Production is frequently presumed to be proportional to upwelling rate, yet high winds can lead to advective losses from continental shelves, where many species at higher trophic levels reside. An idealized mixed-layer conveyor (MLC) model of biological production from constant upwelling winds demonstrated previously that the amount of new production available to shelf species increased with upwelling at low winds, but declined at high winds [Botsford, L.W., Lawrence, C.A., Dever, E.P., Hastings, A., Largier, J., 2003. Wind strength and biological productivity in upwelling systems: an idealized study. Fisheries Oceanography 12, 245–259]. Here we analyze the response of this model to time-varying winds for parameter values and observed winds from the Wind Events and Shelf Transport (WEST) study region. We compare this response to the conventional view that the results of upwelling are proportional to upwelled volume. Most new production per volume upwelled available to shelf species occurs following rapid increases in shelf transit time due to decreases in wind (i.e. relaxations). However, on synoptic, event time-scales shelf production is positively correlated with upwelling rate. This is primarily due to the effect of synchronous periods of low values in these time series, paradoxically due to wind relaxations. On inter-annual time-scales, computing model production from wind forcing from 20 previous years shows that these synchronous periods of low values have little effect on correlations between upwelling and production. Comparison of model production from 20 years of wind data over a range of shelf widths shows that upwelling rate will predict biological production well only in locations where cross-shelf transit times are greater than the time required for phytoplankton or zooplankton production. For stronger mean winds (narrower shelves), annual production falls below the peak of constant wind prediction [Botsford et al., 2003. Wind strength and biological productivity in upwelling systems: an idealized study. Fisheries Oceanography 12, 245–259], then as winds increase further (shelves become narrower) production does not decline as steeply as the constant wind prediction.     

用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分     

The impact of wave loads and pore-water pressure generation on initiation of sediment transport

The build-up of pore-water pressure by waves can lead to sediment liquefaction and subsequent transport by traction currents. This process was investigated by measuring pore-water pressures both in a field experiment and laboratory wave tank tests. Liquefaction was observed in the wave tank tests. The results suggest that sand is less susceptible than silts to wave-induced liquefaction because of the tendency to partially dissipate pore-water pressures. However, previous studies have determined that pore-water pressures must approach liquefaction before current velocities necessary to initiate transport are reduced. Once liquefaction has occurred more sediment can be transported.     

中国近海海区污损生物研究现状及展望

近海海区通常是指离岸3n mile以上或水深超过25m的水域(Relini et al.，1994)；海洋污损生物是海洋环境中栖息或附着在船舶和各种水下人工设施上，对人类经济活动产生不利影响的动物、植物和微生物的总称，它不仅是影响海洋设施安全与使用寿命的重要因素，也是人类开始接触海洋时所关注的问题。本文就污损生物对海洋结构物的影响和我国近海污损生物研究的进展状况、存在问题等方面进行了综合论述，并对未来几年内为研究方向和重点进行了探讨，以期为下一阶段研究工作提供一些具有参考价值的依据和建议。     

黄海海域陆相中生界地震反射特征及靶区优选

利用前人对黄海海域油气勘探成果，作者对北黄海盆地，南黄海盆地北部坳陷陆相中生界进行了研究。结果认为：黄海海域陆相中生界分布广、厚度大、具有良好的生、储、盖组合，是该区下步油气战略性调查的首选目标。并提出北黄海陆相中生界油气勘探应以东部凹陷为主，同时兼顾中部凹陷。南黄海北部坳陷近期油气勘探重点应选择北部凹陷和加大东北凹陷的研究力度。南黄海南部坳陷陆相中生界油气勘探重点放在南五和南七凹陷。     

现场海水中有机污染物光学测量方法研究

介绍了一种用光学方法对海水中的有机污染物（主要包括：叶绿素-a、黄色物质、石油）进行测量的光学系统，该系统可同时测量海水中多种有机污染物的种类和浓度。