海洋微生物学的主要任务

海——微生物栖息的特殊环境。特殊的盐类成分,低温,高压,低浓度的有机物质,植物及动物区系的稀少等组成了微生物生命活动的广阔海洋区域一些主要生态特征。 海洋中最初的微生物学研究,早在上一世纪就已进行了,但足它们局限于较狭小的任务——     

Responses of a Mediterranean soft bottom community to short-term (1993-1996) hydrological changes in the Rhone river

The polychaete fauna of muddy bottoms off the Rhone delta (NW Mediterranean) was seasonally sampled at two sites at 70 m depth, from 1993 to 1996. During this period, five severe flood events occurred. A clustering analysis (distance coefficient of Whittaker) and three way fixed factor ANOVAs (site x season x year) showed strong year-to-year changes in species density and community structure, changes that masked seasonal variations. Total density increased by a factor of 3 and density of most species significantly increased following the first flooding event. Changes in the community structure were due to the successive dominance of a few species. The opportunistic species, such as Cossura sp., Mediomastus sp. and Polycirrus sp., exhibited peaks in density 1-3 months after the flood. These peaks were followed by a drastic decline. For species with a long life span, such as Sternaspis scutata, a slower but continuous increase in density was observed which was maintained for several months. The density fluctuations of these species exhibited a good correlation with river flow with time lags of 1-2 years. The successional dynamics observed are explained according to the geographical origin of the floods and the biology and feeding ecology of species.     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

几种无色硅藻的生理特性及其细胞微细结构的研究

海洋中绝大多数硅藻细胞都有叶绿体,且能利用各种无机盐进行光合作用,称为光合硅藻。但也有不少硅藻尚能利用各种有机物质进行异养生活,据报道约50种左右。其中少数几种完全缺少光合色素,不能进行光合作用,只能利用有机碳或有机氮进行专性异养生活。它们主要生活于沿岸潮间带,特别附生于各种多细胞海藻体上。这类单细胞硅藻,种类不多,但为世界广分布的种类。到目前为止,对无色硅藻的研究报道还很少。本文第一作者在分离培养一种无色硅藻Nitzschia alba Lewin and Lewin作     

青岛—石臼所沿岸潮流特征分析

本文对青岛—石臼所沿岸潮流特征进行了分析。实际观测资料表明,这一带潮流具有典型的往复流性质,并有较明显的不对称性和较大的垂直变化。文中指出涨落潮流不对称性是由于余流和分潮流影响的结果。讨论了不对称性随余流和分潮流的变化和潮流垂直梯度的特征。不对称和垂直梯度对泥沙运动和污染扩散的影响在文中也作了阐述。     

潮流旋转中的倒转现象的探讨

潮流的变化性较潮位要复杂得多。尽管人们多年来一直对它进行观测和研究，但潮流中的许多现象至今仍未得到正确的解释。潮流旋转中的“倒转现象”就是其中之一。所谓倒转现象，就是潮流的旋转方向在一段时间里逆着原来的旋转方向(顺时针或逆时针方向)而旋转，然后再恢复到原来的旋转方向。这种现象在海洋里是经常发生的。     

日平均海面与潮差的关系及其初步动力学解释

文中对若干个站位上的日平均海面和平均潮差进行了谱分析,发现它们的半月变化成分具有明显的线性关系,在指出可能异致这种线性关系的几种原因的同时,用辐射应力的概念并引进摩擦对其作了初步的动力学分析,表明起因于非线性效应的辐射应力亦是导致日平均潮差半月变化的可能原因之一。