Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

物探技术在布敦乌拉隐伏矿产勘查中的应用效果

内蒙古西乌珠穆沁旗布敦乌拉矿区多为第四系覆盖。在该矿区多金属勘查中,依据土壤地球化学圈定的矿化异常布置激电中梯测量,圈定1处激发极化率异常区,长度约890m,宽度约520m,位于已知断层F 1与推断断层F 2之间。采用可控源音频大地电磁测深剖面对该激电异常进行探测,解释视充电率多峰异常与反演电阻率高低跳跃对应区域为矿致异常区。通过钻探验证,发现银多金属矿床资源储量523.93万t,取得了良好的地质找矿效果。     

中尺度数值模式中湿过程参数化的敏感性试验

本文利用一个三维中尺度模式，分别采用两种不同的湿过程处理方案ＦＰＡ和ＮＣＡ模拟同一个洋面冷锋个例，以考察模拟结果对湿过程参数化方案的敏感性。两种方案的区别在于前一种使用了次网格湿对流参数化而后者没有。通过比较分别使用ＦＰＡ和ＮＣＡ方案所进行的模拟ＦＰＳ和ＮＣＳ，发现冷锋结构在两者间出现较大差异。从８５０ hPa等压面|Δθe|等值线图所显示的结构看，ＮＣＳ中冷锋呈现为一长一短两条锋带，而ＦＰＳ中冷锋仅呈现为一条锋带。在相应的冷锋横向垂直剖面中，ＮＣＳ中存在两个相邻的锋面垂直环流，而ＦＰＳ中只有一个。另外，ＮＣＳ中对流不稳定的冷锋区出现垂直运动过分发展的现象，而ＦＰＳ中不存在这个问题。通过与卫星云图比较，我们注意到，ＮＣＳ中尽管出现了垂直运动过分发展的现象，但相对于ＦＰＳ而言，其模拟的冷锋结构与实际较为接近，成功地模拟出云图上显示的双冷锋结构。ＮＣＳ中垂直运动过分发展的一个重要原因是模式中采用的静力平衡近似。ＦＰＳ中次网格对流参数化方案的使用，通过减少甚至消除对流不稳定度，一方面使垂直运动的过分发展倾向受到约束，另一方面也可能使在对流不稳定层结下的锋区环境中本应出现的中尺度结构失去了形成的机会。     

Marine mammals from northeast Atlantic: relationship between their trophic status as determined by delta13C and delta15N measurements and their trace metal concentrations

The relationship between trophic position through delta13C and delta15N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the Northeast Atlantic: striped dolphin Stenella coeruleoalba, common dolphin, Delphinus delphis, Atlantic white-sided dolphin Lagenorhynchus acutus, harbour porpoise Phocoena phocoena, white beaked-dolphin Lagenorhynchus albirostris, grey seal Halichoerus grypus stranded on French Channel and Irish coasts. White-beaked dolphins, harbour porpoises, white-sided dolphins, common and striped dolphins display the same relative and decreasing trophic position, as measured by delta15N values, along both the Irish and French channel coasts, reflecting conservative trophic habits between these two places. Hepatic and renal Cd concentrations were significantly correlated to muscle delta13C and delta15N values while Hg, Zn and Cu did not. These results suggest that Cd accumulation is partly linked to the diet while other factors such as age or body condition might explain Hg, Zn or Cu variability in marine mammals. Combined stable isotope and trace metal analyses appear to be useful tools for the study of marine mammal ecology.     

Types of rural out-migration in Turkey

An analysis of changes in migration patterns since World War II between inner Anatolia and eastern Anatolia in Turkey is presented. Four periods are distinguished, the exploratory period until 1950, seasonal migration until 1960, mass migration up to the present, and marriage migration from 1975 to the present. The linkages between previous and potential migrants are noted. (SUMMARY IN ENG)     

国际构造图:四十年的道路

本文回顾了国际构造图的创意及编制历史,揭示了该项工作对地质科学发展的重要性。它既反映了关于地球演化观念之变化,也反映了固定论地槽—造山带—地台说为活动论板块构造理论所取代的过程。 早在18世纪就有了编制地质图的最初尝试,但基本上是没有时代概念的岩性图。19世纪初,随着真正地质图的出现,地质学才真正成为科学。然而,普通地质图不能充分反映地壳构造     

大气和植被生态及土壤系统水文过程相互作用的一些研究

利用我国测站的降水资料、卫星测得的OLR和高云量资料、SST和137°E次表层海温资料以及NCEP/NCAR再分析资料,分析了东亚夏季风的爆发和北进的年际变化特征及其与热带西太平洋热状态的关系.分析结果表明:当春季热带西太平洋处于暖状态,菲律宾周围对流活动强,在这种情况下,南海上空对流层下层有气旋性距平环流,西太平洋副热带高压偏东,从而使得南海夏季风爆发早;并且,当夏季热带西太平洋也处于暖状态,菲律宾周围对流活动也很强,在这种情况下,西太平洋副热带高压北进时,在6月中旬和7月初存在明显的突跳,从而使得东亚季风雨带在6月中旬明显由华南北跳到江淮流域,并于7月初由江淮流域北跳到黄河流域、华北和东北地区.这将引起江淮流域和长江中、下游夏季风降水偏少,并往往发生干旱,而黄河流域、华北和东北地区的夏季降水正常或偏多.相反,当春季热带西太平洋处于冷状态,菲律宾周围对流活动弱,在这种情况下,南海上空对流层下层有反气旋性距平环流,西太平洋副热带高压偏西,从而使得南海夏季风爆发晚;并且,当夏季热带西太平洋也处于冷状态,菲律宾周围对流活动也很弱,在这种情况下,西太平洋副热带高压北进时,在6月中旬或7月初向北突跳并不明显,而是以渐进式向北移动,从而使得东亚季风雨带一直维持在长江流域和淮河流域.这将引起此两流域夏季风降水偏多,并往往发生洪涝,而黄河流域、华北和东北地区的夏季降水偏少,发生干旱.作者还从非线性多平衡态动力理论说明了菲律宾周围对流活动强弱对西太平洋副热带高压北进时以突跳或渐进式向北移动起到重要作用.