Morphology and molecular characterization of the epiphytic dinoflagellate Prorocentrum cf. rhathymum in temperate waters off Jeju Island, Korea

Prorocentrum spp. are planktonic and/or benthic species. Benthic Prorocentrum species are of primary concern to scientists and the public because some of them are toxic. We established clonal cultures of 3 strains of Prorocentrum species that were collected from the thalli of a macroalga in the coastal waters off Jeju Island, located at the southern end of Korea. The Korean strains of P. cf. rhathymum, which are morphologically almost identical to the Virgin Island strain of P. rhathymum, were different from P. mexicanum because the former dinoflagellate has one simple collar-like spine in the periflagellar area, while the latter dinoflagellate has a 2- or 3-horned spine. In addition, the sequences of the small subunit (SSU) rDNA of the Korean strains were identical to those of the Malaysian and Floridian strains of P. rhathymum, while the sequences of the large subunit (LSU) rDNA of the Korean strains were 0.1–0.9% different from those of the Iranian and Malaysian strains of P. rhathymum. In phylogenetic trees based on the SSU rDNA sequences, the Korean strains of P. rhathymum formed a clade with the Malaysian and Floridian strains of P. rhathymum and the Vietnamese and Polynesian strains of P. mexicanum. However, in phylogenetic trees based on the LSU rDNA sequences, the Korean strains of P. rhathymum formed a clade with the Iranian strain of P. rhathymum and the Spanish and Mexican strains of P. mexicanum. Therefore, the molecular characterization of the Korean strains does not allow us to clearly classify them as P. rhathymum, nor P. mexicanum, although their morphology has so far been reported to be closer to that of P. rhathymum than P. mexicanum and thus we designated them as P. cf. rhathymum.     

Hydrothermal fluid-controlled remagnetization of sedimentary rocks in Korea: Tectonic importance of pervasive Tertiary remagnetization

In order to better understand the tectonic environment of the Korean Peninsula since Cretaceous, a paleomagnetic study was carried out on the Jinan Basin located in southwestern Korea. From the demagnetization of 597 samples, we found that remanence is carried by pyrrhotite and/or magnetite (black siltstone) and hematite (red siltstone), which is a common magnetic mineral assemblage of the rocks remagnetized in Early Tertiary in Korea. All the rock units recorded identical ancient geomagnetic field directions. In particular, paleodirections were better clustered in geographic coordinates, indicating a secondary origin. The presence of abundant silicates as inclusions within Fe-oxides and Fe-sulfides confirms the authigenic origin of the magnetic carriers, precipitated by fluid mediated chemical processes. Compilation of the Korean Cretaceous and Tertiary poles indicates that the Jinan Basin was remagnetized in the Early Tertiary age. In addition, comparison of the Korean Cretaceous and Tertiary poles with those from adjacent major terrains including China and Siberia reveals that a dominant feature of tectonic motions in Korea was a vertical-axis rotation. Sense of vertical-axis rotation was time-dependent, with clear distinction between clockwise rotation in Cretaceous and counter-clockwise rotation in Early Tertiary. Such differences in the mode of vertical axis rotation might be caused by the activities of basin-bounding strike–slip faults in Korea, possibly driven by the changes of subduction regime of the Kula-Pacific plate in Early Tertiary.     

An experimental study of Cl and S distribution between sodalite and fluid

S and Cl distribution between sodalite and fluid was experimentally studied at temperatures of 300–800°C and pressure of 0.5–3 kbar. It is demonstrated that S is preferably distributed into fluid in equilibrium with sodalite of the composition X _S ^Sod > 0.05 throughout the whole temperature range. The distribution of S in the sodalite-fluid system is nonideal. An equation (derived from experimental data) is presented for evaluating the S mole fraction in fluid from the composition of sodalite at a known temperature. The S mole fractions in the fluid are evaluated for sodalite assemblages from nepheline syenites of the Lovozero Massif as being within the range of 0.036–0.23. The S mole fraction in the fluid is proved to increase with increasing mineral formation temperatures.     

Bed morphological changes around a finite patch of vegetation

For the appropriate management and restoration of rivers, isolated vegetation is often a practical means for improving stream habitat and ecology. The effect of a finite vegetation patch on flow and bed morphology in an open channel was investigated using laboratory experiments. The patch containing emergent and submerged vegetation was modeled using circular cylinders and located mid‐channel along a side wall. Several configurations of the patch and submergence ratio (i.e. water depth to the height of vegetation), and two flow conditions (i.e. below and above the sediment motion threshold) were considered. For flows below the sediment motion threshold, erosion occurred primarily on the opposite side of the patch and near the leading edge of the patch. The degree of scouring depth observed in both these regions was affected by the submergence ratio and it increased with the non‐dimensional flow blockage (i.e. the product of the patch density and width). In contrast, for flows above the sediment motion threshold, sediment accumulated within and around the patch due to a reduction in bed shear stress, which was strongly influenced by the flow blockage and the obstruction ratio (i.e. the ratio of patch width to channel width). The eroded area observed within the patch was consistent with the interior adjustment region where the deceleration and diversion of flow occurred through the patch. As the flow blockage increased or as the obstruction ratio decreased, the deposition rate within and behind the patch decreased. Furthermore, the deposition rate increased with an increase in the ratio of flow rate through the patch to total flow rate regardless of the submergence ratio. Copyright © 2014 John Wiley & Sons, Ltd.     

Numerical flow simulation of flush type intake duct of waterjet

Waterjet propulsion system is widely used to thrust high speed marine vessels in excess of 30–35 knots by virtue of high propulsive efficiency, good maneuverability, and less vibration. Since, however, approximately 7–9% of the total power is lost in intake duct due to flow separation, nonuniformity, etc., detail understanding of flow phenomena occurring within intake duct is essential to reduce the power loss. The present work solved 3D incompressible RANS equations on multiblocked grid system of the flush type intake duct of waterjet. The numerical results of surface pressure distributions, velocity vectors, and streamlines were compared with experiments and good agreements were obtained for three jet velocity ratios. Strong suction flow through the inlet was shown and the vortex induced by the separation along the corner of the side wall was clearly shown. Flow separation on the lip was also observed. The location of stagnation point on the lip was well predicted, in accordance with PIV measurement.     

Assessment of acoustic iterative inverse method for bubble sizing to experimental data

Comparative study was carried out for an acoustic iterative inverse method to estimate bubble size distributions in water. Conventional bubble sizing methods consider only sound attenuation for sizing. Choi and Yoon [IEEE, 26(1), 125–130 (2001)] reported an acoustic iterative inverse method, which extracts the sound speed component from the measured sound attenuation. It can more accurately estimate the bubble size distributions in water than do the conventional methods. The estimation results of acoustic iterative inverse method were compared with other experimental data. The experimental data show good agreement with the estimation from the acoustic iterative inverse method. This iterative technique can be utilized for bubble sizing in the ocean.     

Effects of BPA and DES on longchin goby (Chasmichthys dolichognathus) in vitro during the oocyte maturation

This study was performed in order to assess whether bisphenol (BPA) and diethylstilbestrol (DES) had agonistic or antagonistic effects on oocyte maturation using marine fish. We tested the effects of these chemicals on in vitro maturation, germinal vesicle breakdown (GVBD), assay using oocytes from the longchin goby, Chasmichthys dolichognathus. During the maturation process, low concentrations of BPA and DES triggered GVBD depending on the stage of oocyte development; BPA at 0.044 nM and DES at 0.037, 0.37, and 3.73 nM induced GVBD in 0.82-0.88 mm diameter oocytes (germinal vesicle located near the center of oocytes). In 0.76-0.80 mm diameter oocytes (fully vitellogenic oocytes), BPA induced GVBD at relatively higher concentrations (4.38, 43.8, and 438 nM). In 0.86-0.90 mm diameter oocytes, BPA and DES had no observable effect on GVBD at the concentrations tested. Oocytes with diameters between 0.82 and 0.88 mm appeared to be more sensitive to these chemicals. Moreover, our results showed that BPA and DES did not inhibit GVBD.     

Update of the precision geoid determination in Korea

From the late 1990s, many studies on local geoid construction have been made in South Korea. However, the precision of the previous geoid has remained about 15 cm due to distribution and quality problems of gravity and GPS/levelling data. Since 2007, new land gravity data and GPS/levelling data have been obtained through many projects such as the Korean Land Spatilaization, Unified Control Point and Gravity survey on the Benchmark. The newly obtained data are regularly distributed to a certain degree and show much better improvement in their quality. In addition, an airborne gravity survey was conducted in 2008 to cover the Korean peninsula (South Korea only). Therefore, it is expected that the precision of the geoid could be improved. In this study, the new South Korean gravimetric geoid and hybrid geoid are presented based on land, airborne, ship‐borne, altimeter gravity data, geopotential model and topographic data. As for the methodology, the general remove‐restore approach was applied with the best chosen parameters in order to produce a precise local geoid. The global geopotential model EGM08 was used to remove the low‐frequency components using degree and order up to 360 and the short wavelength part of the gravity signal was dealt with by using the Shuttle Radar Topography Mission data. The parameters determined empirically in this study include for Stokes’ integral 0.5° and for Wong‐Gore kernel 110–120°, respectively and 10 km for both the Bjerhammar sphere depth and attenuation factor. The final gravimetric geoid in South Korea ranges from 20–31 m with a precision of 5.45 cm overall compared to 1096 GPS/levelling data. In addition, the South Korean hybrid geoid produces 3.46 cm and 3.92 cm for degrees of fitness and precision, respectively and a better statistic of 2.37 cm for plain and urban areas was achieved. The gravimetric and hybrid geoids are expected to improve further when the refined land gravity data are included in the near future.     

Fossil diatom assemblages as paleoecological indicators of paleo-water environmental change in the Ulleung Basin,East Sea,Republic of Korea

The fossil diatom assemblage record from two sediment cores obtained from the Ulleung Basin, East Sea, Republic of Korea, revealed changes in the diatom assemblage zones in PG1 and PD3 core samples. The two sediment cores were δC¹⁴ dated and approximately represented the late Pleistocene–Holocene. The analysis of age zones in the PG1 core and PD3 core was assessed based on the frequency of variations, and occurrences of biostratigraphical fossil diatom species. During the Last Glacial Maximum (LGM), the sea level was lower than that at present and the Ulleung Basin became isolated from the Pacific Ocean. As a result, there would have been a limited Tsushima Warm Current (TWC) influence, and salinity would have decreased resulting in increased freshwater and coastal diatoms. The distribution pattern of diatoms presented in the cores was associated with changes in water temperature and salinity and the adding of terrigenous material brought about by the input of freshwater. Changes in the abundance of a tychopelagic diatom, Paralia sulcata, reflected the effect of the water currents. Diatom temperature (Td) values and the ratio of centric/pennate diatoms provided evidence of limited influences of the TWC and freshwater inflow. It is thought that all assemblage zones were influenced by the TWC, which had an important effect on the distribution and composition of fossil diatoms.