Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.     

Initiation of primary cell culture from amphioxus <Emphasis Type="Italic">Branchiostoma belcheri tsingtauense</Emphasis>

Amphioxus, a cephalochordate, is an important model fish for studies in evolution and comparative biology. A successful cell culture from amphioxus tissues in vitro would help understanding some basic issues. To determine the optimal culture conditions for proliferation of amphioxus cells, primary cultures were initiated from buccal cirri, tail, gill, gut and metapleural fold of amphioxus Branchiostoma belcheri tsingtauense. The media tested were L-15, F-12, M 199, MEM, DMEM, PRMI 1640 and LDF, each was supplemented with 20% fetal bovine serum. The optimal conditions include tail tissue cultured in L-15 or F-12 with supplement of 20% FBS and 1.5% NaCl at about 25°C. Supported by Doctoral Initial Fund of Ludong University (No.43304)     

巨野煤田彭庄煤矿首采区减少煤损方法简析

山东鲁能菏泽煤电开发有限公司彭庄煤矿位于巨野煤田北部,是巨野煤田7个生产和在建煤矿（包括梁宝寺、彭庄、郭屯、赵楼、龙崮、万福、郓城等煤矿）的第一对投产的矿井。2006年8月开始试生产,2007年3月正式投产。彭庄煤矿2007年底核实保有资源量为12698．9万t,     

东营市矿产资源开发利用现状与保护措施

以东营市矿产资源总体规划为指导,进行矿产资源开发利用与保护措施研究,在开发利用现状的基础上分析当前存在的矿产资源后备基地紧缺、综合利用水平不高以及过度开采等问题,进而提出促进矿产资源开发利用与保护的措施,以便更好地为东营市经济建设和社会发展服务。     

雄性半滑舌鳎雄激素受体基因的克隆与表达分析

利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.     

全球板块运动背景场及ITRF2005VEL的建立

简要分析了最新的国际地球参考框架ITRF2005实现的基本情况及其相对于ITRF2000的改进,以ITRF2005为参考框架建立了新的全球板块运动模型ITRF2005VEL。建模时,首先根据测站的分布和速度场的精度对测站进行初步剔除,然后采用相似变换的方法对全球ITRF2005测站的速度场数据再进行第2次筛选。根据筛选结果利用最小二乘方法建立了全球板块运动背景场及其运动模型,给出了全球11个主要块体的运动参数。此结果与其他学者建立的模型相比,总体上有很好的一致性,但在个别块体上也有所差异。     

全极化SAR数据反演桥面高度

根据高分辨率SAR图像上建筑区的影像特征, 提出了基于灰度共生矩阵(gray-level cooccurrence Matrix, GLCM)纹理分析的建筑区提取方法, 该方法由初步定位和边界调整2个步骤组成, 均遵循特征计算、基于Bhattacharyya距离的特征选择和KNN分类流程, 所不同的是2个步骤中分别采用了逐块和逐点计算纹理特征的方式以兼顾纹理分析的效率和准确性。文中对不同SAR传感器获取的图像进行了实验。实验结果表明, 选用具有最大Bhattacharyya距离值的3或4个特征可以获得较好的初步定位结果, 建筑区的检测率超过80%, 虚警率低于10%;随着边界调整的进行, 检测到的建筑区边界逐渐接近于真实边界。实验结果验证了该算法的有效性。     

3DGIS在公路设计工程中的应用

为了优化公路设计方法,探索了3DGIS技术在公路设计工程方面的应用。从公路设计中的道路选线优化、新设计道路的三维可视化以及土石方工程量的计算等几个方面研究了数字高程模型（DEM）与ArcGIS的三维分析模块在公路设计中的应用。再现了带状数字地面模型,为道路的平面选线工作提供了准确快速的坡度、坡向分析方法,通过行车模拟对道路设计效果进行评价。3DGIS技术辅助公路设计,使得公路设计方案更为直观、科学。     

Interaction of linear waves with an array of infinitely long horizontal circular cylinders in a two-layer fluid of infinite depth

The linear water wave scattering and radiation by an array of infinitely long horizontal circular cylinders in a two-layer fluid of infinite depth is investigated by use of the multipole expansion method. The diffracted and radiated potentials are expressed as a linear combination of infinite multipoles placed at the centre of each cylinder with unknown coefficients to be determined by the cylinder boundary conditions. Analytical expressions for wave forces, hydrodynamic coefficients, reflection and transmission coefficients and energies are derived. Comparisons are made between the present analytical results and those obtained by the boundary element method, and some examples are presented to illustrate the hydrodynamic behavior of multiple horizontal circular cylinders in a two-layer fluid. It is found that for two submerged circular cylinders the influence of the fluid density ratio on internal-mode wave forces is more appreciable than surface-mode wave forces, and the periodic oscillations of hydrodynamic results occur with the increase of the distance between two cylinders; for four submerged circular cylinders the influence of adding two cylinders on the wave forces of the former cylinders is small in low and high wave frequencies, but the influence is appreciable in intermediate wave frequencies.     

Crystallographic and NMR studies on sterols from green alga Chaetomorpha basiretorsa Setchell

Chemical investigation of the ethanol extract of the marine green alga Chaetomorpha basiretorsa Setchell led to the isolation of a new sterol stigmast-4,28-dien-3α,6β-diol 1 in addition to the five known sterols of β-lawsaritol 2, saringosterol 3, 24-hydroperoxy-24-vinyl - cholesterol 4, β-stigmasterol 5, 29-hydroxystigmasta-5, 24 （28） -dien-3β-ol 6. Compounds were isolated by normal phase silica gel and Sephadex LH - 20 gel colum chromatography, reverse phase HPLC and recrystalization. Their structures were elucidated by spectroscopic methods including MS, IR 1D/2D NMR and X-ray analysis. Cytotoxicity of compounds was screened by using the standard MTF method. All these compounds were isolated from the green alga Chaetomorpha basiretorsa Setchell for the first time and they were inactive （50% inhibitory concentration was greater than 10 μg/cm^3） against KB, Bel -7402, PC - 3M, Ketr 3 and MCF-7 cell lines.