Indications of P450 monooxygenase activities in beluga (Delphinapterus leucas) and narwhal (Monodon monoceros) from patterns of PCB, PCDD and PCDF accumulation

Selective accumulation of PCDDs, PCDFs and PCB congeners was investigated in beluga (Delphinapterus leucas), narwhal (Monodon monoceros) and several species at the same trophic level in two areas of Canada. There was a much higher relative abundance of meta-para-unsubstituted PCBs in the cetaceans compared with other species. This suggests that activity of cytochrome P450 monooxygenase enzymes causing CYP2B-type metabolism was relatively low in beluga and narwhal. Lower relative levels of 2,3,7,8-TCDD occurred in beluga from both areas, and selective reduction of toxic non-ortho PCBs occurred in the highly PCB-contaminated St Lawrence beluga. These compounds are potent inducers of cytochrome P450 CYP 1A-type enzymes, suggesting that such an enzyme with the capability of metabolizing TCDD-like substrates is present in beluga and narwhal. SIMCA principal components analysis of PCB congener patterns showed that the two cetaceans were in a statistically significant different class than the other species. Variables (congeners) with the greatest separation power between classes were mainly those defined by the selective metabolism rules.     

Category, narrative, and value in the governance of small-scale fisheries

Since the 1970s, small-scale fisheries have had an important place in fisheries social science and in fisheries management. While there has been substantial discussion of what constitutes the category of small-scale fisheries, its considerable ambiguity is nevertheless often passed over. This paper argues that while the category of scale fisheries can be best understood in terms of scale, the underlying reason for the power of the category lies in the values of social justice and ecological sustainability that it has come to represent in response to dominant high modern narratives of change. Fisheries governance may better be served by prioritising these values rather than by making a fetish out of small-scale fisheries.     

Isolation of differentially expressed genes from contaminant exposed European flounder by suppressive, subtractive hybridisation

Commercially farmed European flounder (Platichthys flesus) were treated with benzo[a]pyrene or cadmium under controlled laboratory conditions. In addition, animals were collected from the polluted Tyne and relatively clean Alde estuaries in the UK. Potential differentially expressed genes were isolated from liver tissue by means of suppressive, subtractive hybridisation. A total of 284 different mRNAs were identified as having the potential for being differentially expressed, of which 84 could be tentatively identified by homology with database sequences. These clones will form part of a flounder cDNA array for validation and future applications, with full identification being pursued for those expressed sequence tags for which differential expression can be confirmed by array technology.     

Endocrine disruption in a marine amphipod? Field observations of intersexuality and de-masculinisation

Intersexuality has been found in both males and females of the marine/estuarine amphipod, Echinogammarus marinus, at polluted and reference sites in East Scotland. Polluted sites had significantly more intersex specimens than reference sites, however the cause of intersexuality is unclear. Discriminant analysis of morphometric data showed that normal male specimens from the most polluted site resembled pooled intersex males, suggesting that subtle endocrine disruption (ED) maybe occurring in these otherwise apparently normal males. The main discriminating character was gnathopod size, recognised to be under androgenic gland control. The association of distinctive morphometry with intersexuality may provide a new approach to biomarkers of ED in crustaceans. The opportunities for other novel biomarkers, for example biochemical or behavioural markers, may also be explored through study of intersex animals.     

A Review of Some Environmental Issues Affecting Marine Mining

This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested.     

Applied social area analysis: Defining and evaluating areas for urban neighbourhood councils

Urban neighbourhood councils, neither statutorily established nor widely initiated in England, are seen to possess a number of advantages over existing forms of representative participation in relation to the equitable distribution of resources. In Portsmouth a community area perception survey and principal components analysis of census data were undertaken to produce synthesised ‘best fit’ social sub-areas upon which to base neighbourhood councils. These areas were then analysed in terms of their relevance and appropriateness, terms specifically defined, along four scale dimensions. An original contention that for maximum effectiveness neighbourhood councils should be established to cover all urban residential areas was confounded by the manifest irrelevance of the neighbourhood council concept in certain socio-spatial contexts.     

Correlating specific conductivity with total hardness in limestone and dolomite karst waters

Under field conditions modern digital conductivity meters give standardized, rapid and reproducible measurements. Here we investigate the accuracy of their estimates of the composition of karst waters, as total hardness (TH, as mg/L CaCO₃) for limestone and dolomite. These are the fundamental measures of process in carbonate karst geomorphology. PHREEQC theoretical curves for the dissolution of pure calcite/aragonite and dolomite in water at 25 °C are compared with water analyses from karst studies worldwide. Other principal ions encountered are sulphates, nitrates and chlorides (the ‘SNC’ group). From carbonate karsts, 2309 spring, well and stream samples were divided into uncontaminated (SNC < 10%), moderately contaminated (10 < SNC < 20%), and contaminated (SNC > 20%) classes. Where specific conductivity (SpC) is less than 600 µS/cm, a clear statistical distinction can be drawn between waters having little contamination and substantially contaminated waters with SNC > 20%. As sometimes claimed in manufacturers' literature, in ‘clean’ limestone waters TH is close to ¹/2SpC, with a standard error of 2–3 mg/L. The slope of the best‐fit line for 1949 samples covering all SNC classes where SpC < 600 µS/cm is 1·86, very close to the 1·88 obtained for clean limestone waters; however, the value of the intercept is ten times higher. The regression line for clean limestone waters where SpC > 600 µS/cm helps to distinguish polluted waters from clean waters with possible endogenic sources of CO₂. In the range 250 < SpC < 600 µS/cm, dolomite waters can be readily distinguished from limestone waters. Copyright © 2005 John Wiley & Sons, Ltd.