军曹鱼不同组织5种同工酶的研究

采用聚丙烯酰胺垂直凝胶电泳法对军曹鱼的几种组织 (脑、心肌、肾、肌肉、性腺、肝、血液、胰、脾 )的 5种同工酶 (LDH、MDH、ME、POD和EST)进行了分析 ,结果表明 :5种同工酶在军曹鱼中都具有明显的组织特异性 ,这些特异性与其组织特定生理功能相关。与其他硬骨鱼类相比 ,军曹鱼的酶谱简单得多 ,这可能与其种属多样性相关。     

Estimation of land production and its response to cultivated land conversion in North China Plain

Food safety and its related influencing factors in China are the hot research topics currently, and cultivated land conversion is one of the significant factors influencing food safety in China. Taking the North China Plain as the study area, this paper examines the changes of cultivated land area using satellite images, estimates land productivity from 1985 to 2005 using the model of Estimation System for Land Productivity (ESLP), and analyzes the impact of cultivated land conversion on the land production. Compared with the grain yield data from statistical yearbooks, the results indicate that ESLP model is an effective tool for estimating land productivity. Land productivity in the North China Plain showed a slight decreasing trend from 1985 to 2005, spatially, increased from the north to the south gradually, and the net changes varied in different areas. Cultivated land area recorded a marginal decrease of 8.0 × 10⁵ ha, mainly converted to other land uses. Cultivated land conversion had more significant negative impacts on land production than land productivity did. Land production decreased by about 6.48 × 10⁶ t caused by cultivated land conversion between 1985 and 2005, accounting for 91.9% of the total land production reduction. Although the land productivity increased in Anhui and Jiangsu provinces, it can not offset the overall adverse effects caused by cultivated land conversion. Therefore, there are significant meanings to control the cultivated land conversion and improve the land productivity for ensuring the land production in the North China Plain.     

Preface: Value and dynamics of salt lakes in a changing world

正Thestatementthattheworld’secosystemsare rapidly deteriorating due to human intervention and global warming is nowadays commonplace. Some o the ecosystems most heavily impacted are inland sal lakes. The salt lakes are among the most valuable and fascinating ecosystems on Earth, and their study has both basic scientifi c interest as well as applied aspects     

Molecular cloning of the heat shock protein 20 gene from Paphia textile and its expression in response to heat shock

P. textile is an important aquaculture species in China and is mainly distributed in Fujian, Guangdong, and Guangxi Provinces. In this study, an HSP20 c DNA designated Pt HSP20 was cloned from P. textile. The full-length c DNA of Pt HSP20 is 1 090 bp long and contains a 5′ untranslated region(UTR) of 93 bp, a 3′ UTR of 475 bp, and an open reading frame(ORF) of 522 bp. The Pt HSP20 c DNA encodes 173 amino acid residues and has a molecular mass of 20.22 k Da and an isoelectric point of 6.2. Its predicted amino acid sequence shows that Pt HSP20 contains a typical α-crystallin domain(residues 77–171) and three polyadenylation signal-sequences at the C-terminus. According to an amino acid sequence alignment, Pt HSP20 shows moderate homology to other mollusk s HSPs. Pt HSP20 m RNA was present in all of the test tissues including the heart, digestive gland, adductor muscle, gonad, gill, and mantle, with the highest concentration found in the gonad. Under the stress of high temperature, the expression of Pt HSP20 m RNA was down-regulated in all of the tissues except the adductor muscle and gonad.     

利用双阳台水管观测资料检测地球自由振荡

基于双阳台水管倾斜仪的数字化观测资料,利用功率谱密度估计方法,在没有对资料进行去固体潮处理的情况下,准确获得了2004年12月26日苏门答腊大地震激发的0S3～0S31基频球型自由振荡。并与PREM模型的理论自由振荡周期进行了对比,发现实测振荡周期与PREM预测的振荡周期相吻合,除0S3、0S4振型的观测周期和PREM模型理论周期的相对误差大于0.3%外,其他振型的观测周期和PREM模型理论周期的相对误差大都集中在0.1%左右。同时还检测到了5个谐频球型振荡和8个环型振荡。     

野外型断层活动连续监测系统

介绍野外型断层活动连续监测系统的组成、布设及主要技术指标。     

基于MapX和VC++的地理信息监控系统的设计与实现

在VC++开发环境下,利用GIS开发工具Mapinfo的MapX组件,设计地理信息监控系统。通过电瓶车信息采集系统客户端软件的实际运用证明了这种方法的实用性,实现了传输数据、地图控制、数据管理等主要功能。     

LHRH-A和hCG对南方鲶血清促性腺激素水平的影响

运用放射免疫分析法测定了促黄体释放激素类似物 (LHRH A)和人绒毛膜促性腺激素(hCG)对南方鲶血清促性腺激素 (GTH)水平的影响 ,结果表明 :LHRH A和hCG都能提高南方鲶一龄幼鱼血清GTH水平 ,hCG与LHRH A结合注射后血清GTH水平显著高于单独注射hCG或LHRH A后的血清GTH水平。LHRH A和hCG结合注射可诱导性成熟南方鲶排卵 ,同时排卵过程中伴随着血清GTH水平的急剧上升 ,在排卵后迅速下降 ;注射同样剂量的药物而未排卵的血清GTH水平虽有升高 ,但升高的幅度不及排卵鱼。这种血清GTH水平的急剧增高对诱导南方鲶卵母细胞最后成熟和排卵具有重要作用。     

Study on application of polyenzyme method to offal of Harengula zunasi

The new polyenzyme method for making gravy fromHarengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50°C, alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, and flavorase concentration of 2.0%, for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3% of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5%, total and amino nitrogen being respectively 1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy fromHarengula zunasi offal. In addition, inorganic elements, the phosphorus content is the highest. This project was aided financially by the Guangdong Science and Technology Office (No. 2KM06002S).     

Vegetation cover variation in the Qilian Mountains and its response to climate change in 2000–2011

An understanding 0f variati0ns in vegetati0n c0ver in resp0nse t0 climate change is critical f0r predicting and managing future terrestrial ec0system dynamics. Because scientists anticipate that m0untain ec0systems will be m0re sensitive t0 future climate change c0mpared t0 0thers, 0ur 0bjectives were t0 investigate the impacts 0f climate change 0n variati0n in vegetati0n c0ver in the Qilian M0untains （QLM）, China, between 2000 and 2011. T0 acc0mplish this, we used linear regressi0n techniques 0n 250-m MODIS N0rmalized Difference Vegetati0n Index （NDVI） datasets and mete0r0l0gical rec0rds t0 determine spati0temp0ral variability in vegetati0n c0ver and climatic fact0rs （i.e. temperature and precipitati0n）. Our results sh0wed that temperatures and precipitati0n have increased in this regi0n during 0ur study peri0d. In additi0n, we f0und that gr0wing seas0n mean NDVI was mainly distributed in the vertical z0ne fr0m 2,700 m t0 3,600 m in elevati0n. In the study regi0n, we 0bserved significant p0sitive and negative trends in vegetati0n c0ver in 26.71% and 2.27% 0f the vegetated areas. C0rrelati0n analyses indicated that rising precipitati0n fr0m May t0 August was resp0nsible f0r increased vegetati0n c0ver in areas with p0sitive trends in gr0wing seas0n mean NDVI. H0wever, there was n0 similar significant c0rrelati0n between gr0wing seas0n mean NDVI and precipitati0n in regi0ns where vegetati0n c0ver declined thr0ugh0ut 0ur study peri0d. Using spatial statistics, we f0und that veeetati0n c0ver freauentlvdeclined in areas within the 2,500-3,100 m vertical z0ne, where it has steep sl0pe, and is 0n the sunny side 0f m0untains. Here, the p0sitive influences 0f increasing precipitati0n c0uld n0t 0ffset the drier c0nditi0ns that 0ccurred thr0ugh warming trends. In c0ntrast, in higher elevati0n z0nes （3,900-4,500 m） 0n the shaded side 0f the m0untains, rising temperatures and increasing precipitati0n impr0ved c0nditi0ns f0r vegetati0n gr0wth. Increased precipitati0n als0 facilitated vegetati0n gr0wth in areas experiencing warming trends at l0wer elevati0ns （2,000-2,400 m） and 0n l0wer sl0pes where water was m0re easily c0nserved. We suggest that spatial differences in variati0n in vegetati0n as the result 0f climate change depend 0n l0cal m0isture and thermal c0nditi0ns, which are mainly c0ntr0lled by t0p0graphy （e.g. elevati0n, aspect, and sl0pe）, and 0ther fact0rs, such as l0cal hydr0l0gy.