Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
The objective of this study is to efficiently extract detailed information about various man-made targets in oriented built-up areas using polarimetric synthetic aperture radar (POLSAR) images. This paper develops an improved approach for building detection by utilizing Two-Dimensional Time-Frequency (2-D TF) decomposition. This method performs outstandingly in distinguishing between man-made and natural targets based on the isotropic behaviors, frequency-sensitive responses, and scattering mechanisms of objects. The proposed method can preserve the spatial resolution and exploit the advantages of TF decomposition; specifically, the exact outlines of buildings can be effectively located, and more types of features (e.g., flat roofs, roads, and walls that are oblique to the radar illumination) can be distinguished from forests in complex built-up areas by 2-D TF decomposition. The coarser-resolution subaperture images that are produced in the azimuth direction, which correspond to different looking angles, are beneficial for detecting man-made structures with main scattering centers oriented at oblique angles with respect to the radar illumination. In the range direction, the obtained subaperture images, which correspond to various observation frequencies, can be helpful in distinguishing flat roofs and roads from forests. This method was successfully implemented to analyze both NASA/JPL L-band AIRSAR and L-band EMISAR data sets. The building detection results of the proposed method exhibit a significant improvement over those of other methods and reach an overall accuracy over 80%, with approximately 20% higher than the accuracies of K-means clustering and the entropy/alpha-Wishart classifier and approximately 10% higher than the accuracy of the support vector machine method. Moreover, building details can be precisely detected, obliquely oriented buildings can be identified, and the distinction between buildings and forests is significantly improved, as both visually and statistically indicated. This method is highly adaptable and has substantial application value. 相似文献
A Wind stress–Current Coupled System (WCCS) consisting of the HYbrid Coordinate Ocean Model (HYCOM) and an improved wind stress algorithm based on Donelan et al. [Donelan, W.M., Drennan, Katsaros, K.B., 1997. The air–sea momentum flux in mixed wind sea and swell conditions. J. Phys. Oceanogr. 27, 2087–2099] is developed by using the Earth System Modeling Framework (ESMF). The WCCS is applied to the global ocean to study the interactions between the wind stress and the ocean surface currents. In this study, the ocean surface current velocity is taken into consideration in the wind stress calculation and air–sea heat flux calculation. The wind stress that contains the effect of ocean surface current velocity will be used to force the HYCOM. The results indicate that the ocean surface velocity exerts an important influence on the wind stress, which, in turn, significantly affects the global ocean surface currents, air–sea heat fluxes, and the thickness of ocean surface boundary layer. Comparison with the TOGA TAO buoy data, the sea surface temperature from the wind–current coupled simulation showed noticeable improvement over the stand-alone HYCOM simulation. 相似文献