Application of the non-seismic geophysical method in the deep geological structure study of Benxi-Huanren area

Based upon 1:200,000 regional gravity and aeromagnetic data, gravity and aeromagnetic slice maps were obtained through fast Fourier transformation (FFT) upward continuation. A comparison between gravity and aeromagnetic slice maps at different depths combined with regional geological data and Magneto-Telluric sounding reveals the deep geological structure of Benxi-Huanren area and provides important information for study of the deep geological evolution process of Benxi-Huanren area. Yanshanian granitic pluton is widely distributed in this area and tends to be continuous toward depth. Significant metallogenic areas, such as Dataigou and Nanfen areas, lie above the buried granitic pluton. The Paleoproterozoic strata distribution area and the Archean strata distribution area have similar characteristics in terms of gravity anomaly. The iron-bearing formation in Anshan area is Archean supracrustal rock which was not engulfed by Archean granite. The thickness of Liaoji paleo-rift can be up to 10 km, whose lower part was intruded by Yanshanian granites. The basement of the Liaoji rift was remelted during granite intrusion in Yanshanian period.     

便携式高频地波雷达海流探测长周期工作性能试验

2013年1月29日至3月15日在台湾海峡西南部海域进行了OSMAR-S100便携式高频地波雷达与浮标观测海流数据的长周期对比试验,验证了雷达系统在探测海流方面的准确性、可靠性和稳定性.通过实测海流与雷达矢量流的复相关分析,选定3 m层的海流为对比代表层.试验期间实测流速为0.0~120.0 cm/s,雷达海流有效探测区内的矢量流流速、流向的观测误差较小,能够满足实时监测海洋表层流的需要,高精度区流速、流向的均方根误差分别为9.1 cm/s和24.8°,边缘区的均方根误差为13.3~24.8 cm/s和39.4°~39.6°,与国内外达到业务化运行要求的同类产品实际观测精度相当.     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

Impact factors identification of spatial heterogeneity of herbaceous plant diversity on five southern islands of Miaodao Archipelago in North China

Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands.     

华南埃迪卡拉(震旦)系顶部地层划分及与寒武系界线FAD分子的选择

"雪球事件"以来,早期生命呈现多层次、大规模的加速演化,到寒武纪前夕,生命大爆发达到高潮;在生命剧烈演替的同时,地球环境标志也发生着响应,碳同位素剧烈漂移,并且与各个生命演化节点相对应。但是在划分最重要的地质界线,埃迪卡拉(震旦)系——寒武系界线及界线层型和点位("金钉子",GSSP)确立的问题上迄今仍存在着较大的分歧,以遗迹化石Treptichnus pedum作为该GSSP的标志化石,因其跨系、跨阶产出,生物延限长,因而在全球的生物地层划分对比中(特别在西伯利亚和中国华南地区)一直质疑声不断,而且以遗迹化石作为标志化石本身也具有一定的缺陷。中国华南地区两类古老的宏体化石——矿化管状的Cloudina和条带状的Shaanxilithes在全球范围内可以很好的对比,并且这两种实体化石的出现都对应着碳同位素的明显正漂移。本文通过研究华南震旦系顶部化石带的地层分布和地理分布,综合分析了其时空分布特点和系统演化意义,深入探讨了Cloudina和Shaanxilithes作为寒武系底界标志化石所具备的潜力,对埃迪卡拉(震旦)纪—寒武纪地层界线划分和层型选择提出了更为理想的标准。     

Tectono–Thermal Evolution, Hydrocarbon Filling and Accumulation Phases of the Hari Sag, in the Yingen–Ejinaqi Basin, Inner Mongolia, Northern China

This work restored the erosion thickness of the top surface of each Cretaceous formations penetrated by the typical well in the Hari sag, and simulated the subsidence burial history of this well with software BasinMod. It is firstly pointed out that the tectonic subsidence evolution of the Hari sag since the Cretaceous can be divided into four phases: initial subsidence phase, rapid subsidence phase,uplift and erosion phase, and stable slow subsidence phase. A detailed reconstruction of the tectonothermal evolution and hydrocarbon generation histories of typical well was undertaken using the EASY R_0% model, which is constrained by vitrinite reflectance(R_0) and homogenization temperatures of fluid inclusions. In the rapid subsidence phase, the peak period of hydrocarbon generation was reached at c.a.105.59 Ma with the increasing thermal evolution degree. A concomitant rapid increase in paleotemperatures occurred and reached a maximum geothermal gradient of about 43-45℃/km. The main hydrocarbon generation period ensued around 105.59-80.00 Ma and the greatest buried depth of the Hari sag was reached at c.a. 80.00 Ma, when the maximum paleo-temperature was over 180℃.Subsequently, the sag entered an uplift and erosion phase followed by a stable slow subsidence phase during which the temperature gradient, thermal evolution, and hydrocarbon generation decreased gradually. The hydrocarbon accumulation period was discussed based on homogenization temperatures of inclusions and it is believed that two periods of rapid hydrocarbon accumulation events occurred during the Cretaceous rapid subsidence phase. The first accumulation period observed in the Bayingebi Formation(K_1 b) occurred primarily around 105.59-103.50 Ma with temperatures of 125-150℃. The second accumulation period observed in the Suhongtu Formation(K_1 s) occurred primarily around84.00-80.00 Ma with temperatures of 120-130℃. The second is the major accumulation period, and the accumulation mainly occurred in the Late Cretaceous. The hydrocarbon accumulation process was comprehensively controlled by tectono-thermal evolution and hydrocarbon generation history. During the rapid subsidence phase, the paleo temperature and geothermal gradient increased rapidly and resulted in increasing thermal evolution extending into the peak period of hydrocarbon generation,which is the key reason for hydrocarbon filling and accumulation.