DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants.

Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 micrograms/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development.     

Transfer of60Co from midwater squid to sperm whales

Sperm whales are notable squid-eaters. They feed mainly on medium to large-sized cephalopods at midwater levels and defecate near the surface. This suggests the existence of an upward transport of⁶⁰Co by sperm whales from the mesopelagic zone (150–1,200 m). To elucidate this squid-whale route for this artificial radionuclide,⁶⁰Co content was determined in squid and in predator whales captured by commercial whaling. In the Cephalopoda livers⁶⁰Co levels of 30–500 mBq kg^–1 wet were found and in the viscera of Odontoceti (toothed whales) 15–40 mBq kg^–1 wet. About 0.3% of⁸⁰Co ingested was estimated to be retained in a 23-year-old male sperm whale. In the livers of Bryde's whales,⁶⁰Co levels of 40–80 mBq kg^–1 wet were detected, but not in euphausiids and sardines, their possible prey. The level of Co in sperm whales was nearly the same as in Bryde's whales. Specific radioactivity⁶⁰Co/⁵⁹Co in mBq µg^–1 was several times higher in sperm whale (1.1–1.6) than in cephalopods (0.19–0.77). Eating prey with a high content of⁶⁰Co in the 1960's may have contributed to the present body burden in sperm whales with a long-life span. However, the origin of⁶⁰Co in Bryde's whales is unknown.     

南海囊糠虾亚科的初步研究

囊糠虾亚科Gastrosaccinae 包括的种数并不很多，但有些种数量相当大，是经济鱼虾的天然饵料，在海洋食物链中占较重要的地位。例如黄海的漂浮囊糠虾Gastrosaccus pelagicus Ii 数量很大，普遍发现于底层鱼类胃含物中；此种及其他一些量大的种又可用作养殖鱼虾的饵料。这一亚科迄今已知共7属69种。本文根据中国科学院海洋研究所1958-60年在南海北部所采的底层浮游生物标本和中国科学院南海海洋研究所1974-81年采自西沙群岛、中沙群岛的标本写成，共发现4属12种，其中6种为中国近海的首次记录。     

三峡工程对长江口区无脊椎动物资源影响的预测

长江大量径流给其河口及邻近海域带来丰富的营养物质,维持了长江河口生态系统的巨大生产力,并使之成为多种重要经济鱼虾类的索饵和产卵场。长江口生态环境的变化,对我国最大、最重要的浙江舟山渔场和江苏吕四渔场的渔业资源有举足轻重的影响。三峡工程的兴建将使长江入海径流量及其季节分配有很大改变,也不可避免地影响到河口和近海的海洋环境、经济动物的组成及资源状况。为研究长江口无脊椎动物资源结构及其分布特征,以及预测三峡工程对自然生态环境的影响,于1985年9月至1986年8月进行了无脊椎动物资源底拖网试捕调查。本文系统整理调查所得资料,结合历史资料进行分析研究。     

Pb2+对青蛤的急性毒性及对其血淋巴液中免疫相关酶活性的影响

采用静态急性毒性实验研究了重金属Pb²⁺对青蛤(Cyclina sinensis)的生物急性毒性效应,测定了在96 h Pb²⁺半致死浓度的1/10(TC组)和1/100(SC组)两个浓度胁迫下,血淋巴液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和溶菌酶(LSZ)活性的变化。结果显示:Pb²⁺的96 h LC50为7.938 mg/L; SC组ACP活性表现为诱导-抑制趋势,除4 d外均与对照组差异显著(P<0.05), TC组为抑制趋势,为3个组中的最低,与对照组差异显著(P<0.05);实验组SC组和TC组的AKP、LSZ活性均表现为前期为诱导中后期受抑制的趋势,与对照组差异显著(P<0.05),且TC组活性始终低于SC组,表现出Pb²⁺的胁迫浓度越高酶活性受到的抑制作用越大。以上结果表明,重金属Pb²⁺对青蛤的毒性级别为高毒级,能造成青蛤免疫相关酶的活性受到抑制,影响青蛤的免疫能力,而且这种抑制作用随着环境中Pb²⁺浓度增加而增加...     

An experimental study on non-linear progressive wave-induced dynamic stresses in seabed

In this study, the dynamic stresses within the seabed induced by non-linear progressive waves were explored through a series of hydraulic model tests on a movable bed within a wave flume. By comparing Stokes’ 2nd-order wave theory with the theory of wave-induced dynamic stresses within the seabed as proposed by Yamamoto et al. [1978. On the response of a poro-elastic bed to water waves. Journal of Fluid Mechanics 87 (1), 193–206.] and Hsu and Jeng [1994. Wane-induced soil response in an unsaturated anisotropic seabed of finite thickness. International Journal for Numerical and Analytical Methods in Geomechanics 18, 785–807], the experimental results show that the pressure on the seabed surface, the pore water pressure within the seabed as well as the vertical and the horizontal stresses are all smaller than their theoretical values. If we were to obtain the characteristics of seabed soil, the analytical solution of Hsu and Jeng [1994. Wane-induced soil response in an unsaturated anisotropic seabed of finite thickness. International Journal for Numerical and Analytical Methods in Geomechanics 18, 785–807] might agree to the simulation of the wave-induced effective stresses and shear stress in the sandy seabed. A different phase shift exists among all the three soil stresses. Their influences on the three dynamic stresses within seabed soil are important for seabed stability, and can be used in the verification of numerical models. In the whole, the non-linear progressive waves and the naturally deposited seabed are found to have a strong interaction, and the behavior of the induced dynamic stresses within the seabed is very complicated, and should be investigated integrally.     

Reconstruction of sea surface dimethylsulfide in the North Pacific during 1970s to 2000s

We proposed an empirical equation of sea surface dimethylsulfide (DMS, nM) using sea surface temperature (SST, K), sea surface nitrate (SSN, μM) and latitude (L, °N) to reconstruct the sea surface flux of DMS over the North Pacific between 25°N and 55°N: ln DMS = 0.06346 · SST − 0.1210 · SSN − 14.11 · cos(L) − 6.278 (R² = 0.63, p < 0.0001). Applying our algorithm to climatological hydrographic data in the North Pacific, we reconstructed the climatological distributions of DMS and its flux between 25 °N and 55 °N. DMS generally increased eastward and northward, and DMS in the northeastern region became to 2–5 times as large as that in the southwestern region. DMS in the later half of the year was 2–4 times as large as that in the first half of the year. Moreover, applying our algorithm to hydrographic time series datasets in the western North Pacific from 1971 to 2000, we found that DMS in the last three decades has shown linear increasing trends of 0.03 ± 0.01 nM year^− 1 in the subpolar region, and 0.01 ± 0.001 nM year^− 1 in the subtropical region, indicating that the annual flux of DMS from sea to air has increased by 1.9–4.8 μmol m^− 2 year^− 1. The linear increase was consistent with the annual rate of increase of 1% of the climatological averaged flux in the western North Pacific in the last three decades.