海带磷酸甘露糖变位酶(PMM)基因的克隆与表达分析

磷酸甘露糖变位酶(PMM)是褐藻胶和岩藻聚糖合成过程中的关键酶之一。本研究利用c DNA末端快速克隆(RACE)技术,获得2条海带PMM基因(Sjpmm1,Sjpmm2)序列。其中,Sjpmm1的开放阅读框(ORF)长759 bp,其编码的Sj PMM1为卤酸脱卤酶(HAD)超家族成员,含252个氨基酸,分子量约为28.51 k Da;而Sjpmm2的ORF长1866 bp,其编码的Sj PMM2属于磷酸己糖变位酶超家族的成员,含621个氨基酸,分子量约为66.49 k Da。海带PMM的二级结构均以?-螺旋为主。进化分析表明,Sjpmm1来自于原始真核生物,而Sjpmm2来源于质体的第一次内共生作用。实时定量PCR分析发现,海带受到高温或低温胁迫时,Sjpmm1和Sjpmm2转录水平上升,以合成岩藻聚糖抵抗环境影响。此外,利用p MAL-c5X载体对Sj PMM1进行体外表达,得到高浓度的可溶性融合蛋白,为后续的Sj PMM功能分析提供基础。     

基于Neuman井流理论确定玄武岩潜水含水层渗透系数的应用——以吉林靖宇煤矿为例

全面分析研究区玄武岩潜水含水层水文地质条件的基础上,对稳定流和非稳定理论确定潜水含水层渗透系数的优缺点进行了对比,认为非稳定井流公式确定玄武岩潜水含水层渗透系数是合理的。Aquifer Test软件基于Neuman井流理论配线法求参原理,运用计算机完成了自动求参,避免了人工配线的不足。该文根据吉林靖宇煤矿研究区玄武岩潜水含水层非稳定流抽水试验,分别用Aquifer Test软件和稳定井流公式进行了求参,并对比分析得出:玄武岩含水层弹性释水作用影响时间较长,利用Neuman潜水井流公式求参可靠,且更逼近现实;用Aquifer Test软件实现了自动求参,具有操作简单规范、可比性好的优点。     

Geochemical and Petrological Studies on the Early Carboniferous Sidingheishan Mafic–Ultramafic Iintrusion in the Southern Margin of the Central Asian Orogenic Belt, NW China

The Sidingheishan mafic-ultramafic intrusion is located in the eastern part of the Northern Tianshan Mountain, along the southern margin of the Central Asian Orogenic Belt in northern Xinjiang autonomous region of China. The Sidingheishan intrusion is mainly composed of wehrlite, olivine websterite, olivine gabbro, gabbro and hornblende gabbro. At least two pulses of magma were involved in the formation of the intrusion. The first pulse of magma produced an olivine-free unit and the second pulse produced an olivine-bearing unit. The magmas intruded the Devonian granites and granodiorites.An age of 351.4±5.8 Ma(Early Carboniferous) for the Sidingheishan intrusion has been determined by U-Pb SHRIMP analysis of zircon grains separated from the olivine gabbro unit. A U-Pb age of 359.2±6.4 Ma from the gabbro unit has been obtained by LA-ICP-MS. Olivine of the Sidingheishan intrusion reaches 82.52 mole% Fo and 1414 ppm Ni. On the basis of olivine-liquid equilibria, it has been calculated that the MgO and FeO included in the parental magma of a wehrlite sample were approximately10.43 wt% and 13.14 wt%, respectively. The Sidingheishan intrusive rocks are characterized by moderate enrichments in Th and Sm, slight enrichments in light REE, and depletions in Nb, Ta, Zr and Hf. The ε_(Nd)(t) values in the rock units vary from +6.70 to +9.64, and initial ~(87)Sr/~(86)Sr ratios range between 0.7035 and0.7042. Initial ~(206)Pb/~(204)Pb, ~(207)Pb/~(204)Pb and ~(208)Pb/~(204)Pb values fall in the ranges of 17.23-17.91,15.45-15.54 and 37.54-38.09 respectively. These characteristics are collectively similar to the Heishan intrusion and the Early Carboniferous subduction related volcanic rocks in the Santanghu Basin, North Tianshan and Beishan area. The low(La/Gd)_(PM) values between 0.26 and 1.77 indicate that the magma of the Sidingheishan intrusion was most likely derived from a depleted spinel-peridotite mantle.(Th/Nb)_(PM)ratios from 0.59 to 20.25 indicate contamination of the parental magma in the upper crust.Crystallization modeling methods suggest that the parental magma of the Sidingheishan intrusion was generated by flush melting of the asthenosphere and subsequently there was about 10 vol%contamination from a granitic melt. This was followed by about 5 vol% assimilation of upper crustal rocks. Thus, the high-Mg basaltic parental magma of Sidingheishan intrusion is interpreted to have formed from partial melting of the asthenosphere during the break-off of a subducted slab.     

Comparative Study of Hydrogen and Carbon Isotopic Composition of Gases Generated from the Pyrolysis of a Peat under Saltwater and Freshwater Conditions

To understand the influence of the diagenetic water medium on the isotopic compositions of thermogenic coalbed gas, both hydrous and anhydrous closed-system pyrolyses were performed at temperatures of 250°C to 650°C on an herbaceous marsh peat. Compared to the results of anhydrous pyrolysis, the hydrocarbon gases generated from hydrous pyrolyses have very different hydrogen isotopic compositions. However, the carbon isotopic compositions of the hydrocarbon gases became only slightly heavier in hydrous pyrolysis, compared to that from anhydrous pyrolysis. With the progress of thermal evolution from peat to a more advanced thermal maturity of vitrinite reflectance values (Ro) of 5.5% during the pyrolysis, the difference in the average δD value increased from 52‰ to 64‰ between the hydrous pyrolysis with saltwater and anhydrous pyrolysis and increased from 18‰ to 29‰ between the hydrous pyrolysis with freshwater and anhydrous pyrolysis, respectively. The difference in the average δ13C value was only 1‰–2‰ between the hydrous and anhydrous pyrolysis. The relationships between the δD values of the generated hydrocarbon gases and Ro values as well as among δD values of the hydrocarbon gas species are established. The close relationships among these parameters suggest that the water medium had a significant effect on the hydrogen isotopic composition and a minimal effect on the carbon isotopic composition of the hydrocarbon gases. The results of these pyrolyses may provide information for the understanding of the genesis of coalbed gas from herbaceous marsh material with the participation of different diagenetic water media.     

基于稳态碎岩方式的PDC钻头设计分析研究

自从20世纪80年代PDC复合片研发成功以来,PDC钻头由于其钻进性能强和耐磨性能好,在世界许多国家的石油钻井和地质勘探中得到了广泛应用,取得了很好的效果。但是,如何进一步提高其在钻探工程中的技术经济指标是一个很迫切的问题。俄罗斯博士Скоромных В. В.教授等对此进行多年研究,提出了稳态破碎岩石方式的思想,即在轴载和扭矩切力形成的合力R与切削面成垂直角度条件下,切入深度h不变时形成的一种碎岩方式。并在此基础上提出了一些设计PDC钻头方面的建议。     

黑龙江省塔河县宝兴沟金矿床中生代侵入岩及其对成矿作用的制约

宝兴沟金矿床为近年来在漠河前陆盆地南缘新发现的大型金矿床。区内发育石英闪长岩、闪长玢岩、花岗细晶岩等侵入岩,矿体主要产于石英闪长岩、闪长玢岩内部及其与二十二站组砂岩的内外接触带中。地球化学研究表明,石英闪长岩具高钾、低钛、富碱特征,花岗细晶岩具高硅、高钾、低钛、富碱特征,二者均为高钾钙碱性系列岩石,富集大离子亲石元素Rb、Ba、Sr等,亏损高场强元素Nb、Ta、Ti和重稀土元素Yb、Y,但二者稀土元素特征和配分曲线差异较大,不具成因联系。矿石与石英闪长岩、闪长玢岩具有相似的微量元素、稀土元素组成和稀土元素配分曲线,表明石英闪长岩、闪长玢岩为成矿提供了热液和成矿物质等,与成矿具有密切的成因联系。LA-ICP-MS锆石U-Pb同位素测年结果显示,石英闪长岩成岩时间为118.13~121.77 Ma,限定了宝兴沟金矿床的形成时间不早于118Ma,为早白垩世,形成于太平洋板块俯冲后的伸展环境。漠河前陆盆地除中-晚侏罗世侵入岩外,早白垩世中酸性侵入岩对区域成矿也具有重要的作用,是今后找矿的重要方向之一。     

季节性低温胁迫下陇东黄土高原油污土壤环境因子对耐冷混合菌场地生态修复的响应

为了揭示季节性低温胁迫下陇东黄土高原油污土壤环境因子对耐冷混合菌场地生态修复的响应机制,利用自主筛选构建的耐冷石油降解混合菌在甘肃省庆阳市庆城县马岭镇长庆油田陇东油泥处理站开展了为期7个月的场地修复实验,采用常规方法测定了不同季节土壤理化特性、酶活性和微生物群落特性等环境指标。结果表明：（1）在季节性低温胁迫下（9-11月）M2组（耐冷混合菌处理组）月平均降解率明显增加（P<0.01）,JZJ+M1组（金盏菊联合常温混合菌处理组）和M2组累计TPH降解率分别为15.37%±3.51%和28.64%±4.12%。（2）M2组土壤脱氢酶和多酚氧化酶在低温季节（LT）活性最高,且温度和处理存在显著交互作用（P<0.01）。在土壤营养元素方面,无论何种处理方式（JZJ+M1和M2）二者含量均为RT（常温季节）高于LT（P<0.05）,同时明显高于CK组（P<0.05）。（3）M2组土壤微生物群落Shannon-Wiener指数和均匀度指数在LT高于RT（P<0.05）。（4）NMDS和Pearson相关性分析结果显示,M2组在低温季节具有较高TPH降解率主要与土壤多酚氧化酶、脱氢酶、单月TPH降解率（μ2）和Shannon-Wiener指数有关,且均呈极显著正相关关系。通过分析环境因子的季节响应,优化场地修复在低温环境的降解条件、加速低温期石油烃降解速率,以期为陇东地区低温耐冷混合菌场地生态修复技术的推广应用提供参考和基础数据资料。     

某水电站某泥石流沟综合治理概述

某水电站某泥石流沟内常年流水,属季节性排洪沟谷,水动力类型为暴雨,沟内松散物源丰富,具有泥石流发生条件。在上游区以拦挡泥石流为主,通过修建拦挡措施,减少形成泥石流的固体物质,在下游区以防水、边坡防护及引流等措施,通过修建溢流坝截断上游水流对沟内堆渣体渗流影响;通过修建排洪渠及排洪洞引流;通过削坡、修坡进行边坡维护加固。确保了导流洞出口及上下行交通洞进出口的施工期安全及运行安全。     

GPS RTK在异形建筑物放样中的应用

针对异形建筑物传统施工放样过程操作复杂、效率低下的问题,该文提出了采用GPS RTK进行异性建筑物施工中的放样工作。以天津市文化中心商业项目为例,利用GPS RTK技术分别对建筑物的主轴线和异形次轴线进行了放样工作,并与全站仪在放样过程和数据精度两个方面进行了对比分析,实现了异型建筑物快速、高效的施工放样。实验结果表明,GPS RTK技术不仅在放样过程中操作简单,节约资金,而且放样精度满足工程要求,相对全站仪来说具有很大优势,有助于提高复杂异形建筑物的施工放样效率。     

新型同径分层止水工艺在地热探采深井的应用

原平大营地热探采结合深井完钻井深3088 m,为探明494~3088 m变质岩地层的水文地质参数和水源补给情况,基于岩心和测井资料划分4个抽水试验段,且试验后需要恢复目的含水层。根据“一孔同径分层止水”原则,设计了管内止水托盘止水、管外止水伞止水的新型同径分层止水工艺。该工艺简化了井身结构,避免了管外投粘土球、注水泥等堵塞含水层的情况。现场实施表明,该工艺止水效果完全满足水文地质抽水试验要求,可为同类工程提供经验和借鉴。