中国对虾选育群体“黄海1号”和野生群体不同组织基因组DNA甲基化的MSAP分析

DNA甲基化在调节动物生长发育和组织分化中发挥了重要作用。本研究主要从酶切、预扩和选扩等方面优化了中国对虾DNA甲基化分析的MSAP技术,给出了适合中国对虾MSAP分析的最近反应程序和体系,并采用该技术分别对中国对虾选育群体"黄海1号"和野生群体的肌肉、鳃和血细胞三种组织基因组DNA的CCGG甲基化水平进行分析。研究结果表明,中国对虾野生群体肌肉、鳃和血细胞的DNA甲基化比例分别为23.1%、22.3%和19.7%,选育群体"黄海1号"肌肉、鳃和血液的甲基化比例分别为21.4%、19.6%和18.9%。鳃组织的DNA甲基化水平在两群体中差异极显著(P <0.01),肌肉间的甲基化水平差异显著(P <0.05),而血细胞中甲基化水平差异不显著(P >0.05)。中国对虾野生群体和"黄海1号"同一组织间的甲基化水平不同(P ≤ 0.05),而不同组织间的甲基化水平也各不相同(P ≤ 0.05)。DNA甲基化多态性分析表明,野生群体和选育群体"黄海1号"的鳃和血细胞组织的甲基化多态性比例变化明显,而肌肉组织甲基化水平较稳定,这些变化趋势与CCGG位点的甲基化和去甲基化有关。     

Experimental research on horizontal bearing behavior of caisson-pile composite foundationEI北大核心CSCD

In this study, four groups of different types of bridge foundation model are tested to research the horizontal bearing behavior of caisson-pile composite foundation in lab based on the Qiongzhou Strait bridge project. The Q-s curve and horizontal ultimate bearing capacity of these four groups of foundations in sandy soil layer are obtained. Pile bending moment and shear force of pile shaft are analyzed in detail. At the same time, the load sharing ratio of caisson and piles is discussed. The results show that the horizontal ultimate bearing capacities of a single caisson foundation are increased by 1.2 times, 1.6 times and 2 times respectively with adding skirts, steel pipe pile, or steel pipe pile and the skirt. The maximum bending moment point is in the middle of the pile shaft, i.e. at about 0.5 m under the mud surface. The horizontal load is borne mainly by the upper caisson foundation and soil layers above the middle upper part of pile. The maximum shear force is found at the joint of pile top of steel pipe and caisson pile, where the reinforcement measures should be taken in practical engineering. The research achievements could provide a better reference for design or construction of caisson-pile composite foundation.     

我国海洋声学探测技术竞争力分析

海洋声学探测技术用于海洋物理及海洋特定目标特性探测,是人类认识海洋、开发和利用海洋丰富资源必不可少的海洋高技术。文中全面介绍了海洋声学探测技术的技术结构以及致力于此技术研究的国内外主要研究机构,着重对比分析了国内外海洋声学探测技术的发展现状与趋势,并总结出我国该领域现存在的问题。结果表明我国海洋声学探测技术水平与国外相差10 a左右,未来需靠长期稳定支持、按需开展研究、加大基础工业投资以及提高材料与工艺水平等措施推进我国海洋声学技术的良性发展。     

半潜式起重平台系泊系统设计与优化研究

近年来,国内兴起了大力发展半潜式起重平台的势头,系泊系统的设计与优化是该类型平台开发中的关键问题之一。采用时域方法对半潜式起重平台系泊系统进行耦合动力分析,研究系泊系统主要参数对其动力响应特性的影响,并给出主要参数的设计优化流程,在此基础上,以能够满足安全校核且性能较优的系泊方案为目标,对该半潜式起重平台的系泊系统进行优化设计,并研究单根系泊缆发生破断对整个系泊系统的影响。结果表明:基于主要参数对系泊性能的影响规律,经优化设计得到的系泊方案,预留的安全欲度适中,能够保障半潜式起重平台在恶劣海况下的生存能力,同时具备较好的经济性。     

������ģ�͵IJ�����С���˹���

??????e????????????????????????????????e????????е??????????????????????????????????ж????????????????????????С????????????????????????????????????????????????????????????????????y???Ч????     

站式全景遥感影像展示方法与应用技术研究

虚拟漫游技术已成功应用到社会各个领域,传统漫游技术如VRML(Virtual Reality Modeling Language)、Quick Time、PTGui(Panorama Tools)等存在集成难度大、建模时间长、投入成本高等问题。本文基于全景相机以及Pano2vr、Dynamic-Photo HDR、Photoshop等软硬件环境,研发搭建了一整套旅游景区全景影像采集与展示平台。该平台具有良好的沉浸性、多视角浏览、实现方法简单、搭建快速等优点,为旅游景点、酒店、历史文化名胜等场景的360度实景展示提供了有效地工程化解决途径。     

城市创伤中心空间选址的层次分析模型

针对城市创伤中心空间选址问题,建立包含四大类、八小类影响因素的层次分析模型,邀请不同领域的专家对构造出的判断矩阵综合打分,得出各因素的相应权重后运用到深圳市创伤中心空间选址的实例研究上。模型中所涉及的空间数据均基于网络分析,更贴合实际情况。同时,考虑到深圳市创伤中心的依托医院相对集中这一特殊情况,通过聚类分析划分区域后再使用层次分析模型选址。优化后的结果将改善深圳市创伤医疗救治资源配置、提高创伤救治效率,为创伤救治机构规划部门提供参考意见。     

Effects of waterborne Cu and Cd on anti-oxidative response,lipid peroxidation and heavy metals accumulation in abalone Haliotis discus hannai ino

The aim of this study was to compare the effects of waterborne copper(Cu) and cadmium(Cd) on survival, anti-oxidative response, lipid peroxidation and metal accumulation in abalone Haliotis discus hannai. Experimental animals(initial weight: 7.49 g ± 0.01 g) were exposed to graded concentrations of waterborne Cu(0.02, 0.04, 0.06, 0.08 mg L-1) or Cd(0.025, 0.05, 0.25, 0.5mg L-1) for 28 days, respectively. Activities of the anti-oxidative enzymes(catalase, CAT; superoxide dismutase, SOD; glutathione peroxidases, GPx; glutathione S-transferase, GST), contents of the reduced glutathione(GSH) and malondiadehyde(MDA) in the hepatopancreas, and metal accumulation in hepatopancreas and muscles were analyzed after 0, 1, 3, 6, 10, 15, 21, 28 days of metal exposure, respectively. Results showed that 0.04 mg L-1, 0.06 mg L-1 and 0.08 mg L-1 Cu caused 100% death of abalone on the 21 st, 10 th and 6th day, respectively. However, no dead abalone was found during the 28-day waterborne Cd exposure at all experimental concentrations. Generally, activities of SOD and GST in hepatopancreas under all Cu concentrations followed a decrease trend as the exposure time prolonged. However, these activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Activities of CAT in all Cu exposure treatments were higher than those in the control. These activities were firstly increased and then decreased to the control level and increased again during Cd exposure. Contents of MDA in hepatopancreas in all Cu treatments significantly increased first and then decreased to the control level. However, the MDA contents in hepatopancreas were not significantly changed during the 28-day Cd exposure. The metals accumulation in both hepatopancreas and muscles of abalone significantly increased with the increase of waterborne metals concentration and exposure time. These results indicated that H. discus hannai has a positive anti-oxidative defense against Cu or Cd. In conclusion, anti-oxidative mechanism in abalone to resist waterborne Cu did not follow the same pattern as that for waterborne Cd.     

小牛煤矿晚二叠世含煤地层沉积环境与聚煤规律

小牛煤矿位于贵州省内水城矿区的格目底向斜东段北翼,含煤地层为晚二叠世龙潭组,含煤性好。通过对小牛煤矿含煤地层及其含煤性的研究,确定了研究区的沉积环境是海陆过渡相中的三角洲沉积类型,并初步建立了沉积模式,龙潭早期和晚期均有较好的聚煤作用发生并叠加,是聚煤的最佳场所,含煤性最好。     

东营凹陷八面河北部斜坡带构造特征及形成机制

研究了东营凹陷八面河北部斜坡带的断裂特征、构造演化特征及其形成机制,明确了该区的断裂展布特点和断层活动性,弄清了北部斜坡带的演化规律,同时分析该区的构造变形特征。结果表明：中生代以后,八面河地区存在2种独立的构造变形系统：一是板块边缘相互作用力;二是到后期由于板块的持续俯冲,地幔底辟作用在岩石圈底面产生的牵引力。八面河北部地区在这2种构造力的综合作用下,不同时期表现出不同的构造变形特征。