寒武系崮山阶三叶虫生物地层新认识

由于Diceratocephalus已在华北的若干地点找到,如莱芜九龙山、淄博黑石寨和泰安大汶口,Dicerato-cephalus三叶虫亚带可以提升为三叶虫带,这样崮山阶便包含了4个三叶虫带(自上而下)Diceratoceph alusarma-tus带、Drepanura premesnili带、Blackwelderia paronai带和Damesella paronai带。     

中国企业R&D国际化:时空格局与区位选择影响因素

基于中国企业海外R&D投资数据和零断尾负二项回归分析方法,探讨了1998—2015年中国企业R&D国际化的时空格局特征,并从东道国角度分析影响其区位选择的主要因素。结果表明：研究期内中国新建海外R&D机构数量快速扩张;海外R&D投资区域分布广泛,呈现分散和集聚并存的特征;以华为为例分析了典型中国企业海外R&D投资的空间布局特征,认为目前华为海外R&D投资呈现集中布局在欧洲,零散分布在亚洲、美洲、非洲的空间特征,主要是由其以全球技术升级和满足多样化的市场需求等R&D国际化的战略动机所决定。回归分析结果表明,中国企业对发达国家和发展中国家进行R&D投资的影响因素有共同点但也存在一定的差异：① 现阶段东道国的R&D需求不是影响中国企业对其进行R&D投资的主要因素;② 代表中国与东道国经济往来密切程度的关联要素是影响中国企业R&D投资的重要决策因素;③ 发达国家吸引中国企业对其进行R&D投资主要是因其创新能力强、R&D资源数量多,而政策及制度供给是影响中国企业对发展中国家进行R&D投资的主要因素;④ 地理距离对中国企业R&D“走出去”的阻碍作用主要体现在对发展中国家的R&D投资中。     

马氏珠母贝TRACP基因的克隆及组织表达

【目的】克隆马氏珠母贝(Pinctada martensii)抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRACP)基因,分析该基因在不同组织中的表达模式。【方法】用RACE技术克隆得马氏珠母贝TRACP基因(PmTRACP),用实时荧光定量PCR分析该基因在外套膜、闭壳肌、足、性腺、珍珠囊、肝胰腺和鳃中的表达。【结果与结论】PmTRACP基因长度为2 034 bp,开放式阅读框972 bp,编码323个氨基酸,5′UTR长度为27 bp,3′UTR长度为1 035 bp。预测PmTRACP分子质量约为36.39 ku,理论等电点为5.97。该基因含有一个钙调神经磷酸酶样磷酸酯酶结构域。PmTRACP与其他物种TRACP的同源性为48%～65%,与长牡蛎(Crassostrea gigas)TRACP的同源性最高,同时D³²、D⁷⁰、Y⁷³、N¹⁰⁸、H²⁰³、H²¹²、H²³⁷、H²³⁹等8个氨基酸活性位点和N¹¹⁴糖基化位点在不同物种TRACP中高度保守。PmTRACP与长牡蛎TRACP的亲缘关系最近。PmTRACP在马氏珠母贝各个组织均有表达,且在肝胰腺和珍珠囊中高表达。     

1997—2017年塔克拉玛干沙漠腹地降水特征

利用塔克拉玛干沙漠腹地塔中气象站1997—2017年逐日和逐时降水资料,分析塔克拉玛干沙漠腹地降水日变化特征、极端强降水特征及其天气背景。结果表明：1997—2017年研究区降水量呈增加趋势、降水日数呈减少趋势,大雨雨量和雨日明显增加,降水呈增强演变。降水多见于6月,最大雨强为8.4 mm·h^-1。降水量日变化呈多峰特征,降水量最大值出现在23：00,06：00是降水频次最多时刻。降水强度和降水频次对降水量作用不同,午后至前半夜强度大,频次少;而后半夜至清晨频次多,强度小。降水以短历时降水为主,其中1～3 h的短历时降水对总降水量贡献率高达61.76%。日降水和小时降水的99百分位强度阈值分别为15.3 mm·d^-1和6.0 mm·h^-1,大于90百分位极端降水量占总降水量贡献率近半。极端强降水天气发生在南疆盆地受北纬40°以南低槽、切变槽或弱的气旋式风场控制地区,南疆盆地提前增湿,民丰850 hPa比湿接近或超过10 g·kg^-1的背景下,降水连续性较差,多中小尺度引发局地短时降水。     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Research on the Optimization of Capital for the Governance of Bulk Coal in Beijing-Tianjin-Hebei Region

Beijing, Tianjin and Hebei each contributed to the comprehensive governance of bulk coal to treat bulk coal pollution in a mutually beneficial way in 2017. The cooperative game theory is used in this paper to study the environmental benefits and cost effectiveness brought about by this comprehensive governance strategy, primarily focusing on the issue of how to maximize the environmental benefits by choosing an appropriate strategy since the benefits to Beijing, Tianjin and Hebei are closely related. Therefore, the linear optimization, game theory and Shapley value method in the cooperative game model are used to find the ways to minimize the total governance cost of bulk coal in the three areas. In addition, the issues of how to carry out rational distribution and transfer of governance capital among the three places are explored according to the actual amounts of consumption of bulk coal, the influence of the coal burning on the PM_2.5 and the actual cost of coal governance in Beijing, Tianjin and Hebei in 2017. The results show that the governance task in Hebei Province is the most onerous, and requires more investment than the other two cities. Thus, it requires the support from other two cities, with the amount of increased capital required of about 600 million Yuan. At the same time, the cost saved after optimization in Tianjin is calculated to be the largest, which thus can be adjusted appropriately and allocated to Hebei for the governance of bulk coal. The model constructed in this paper can not only be used to solve the issues related to bulk coal consumption in Beijing, Tianjin and Hebei, but also to carry out the effective distribution of capital, by which a win-win scenario among the three places can be achieved.     

中国半干旱草原施氮梯度下的土壤激发效应（英文）

为了探究施氮对土壤有机质(SOM)的激发效应,本研究在施氮梯度样地(0、4和16 g N m^–2 yr^–1)上进行了¹³C标记葡萄糖的原位添加实验,并对土壤CO_2排放量和磷脂脂肪酸(PLFA)含量进行了测定。研究发现施氮降低了土壤CO_2排放、土壤PLFA含量以及土壤真菌细菌比。在0 g N m^–2 yr^–1样地上葡萄糖添加导致的正向激发效应最强,同时4 g N m^–2 yr^–1样地释放的葡萄糖来源的碳最多。因此,施氮减少了土壤中SOM转化产生的CO_2,微生物碳的来源由SOM转变为添加的易分解碳。本研究采样早期土壤微生物生物量和群落结构稳定,表明该草原存在"表观激发效应",因此未来研究应着重对微生物功能的多样性进行探讨。     

Soil Respiration Dynamics and Influencing Factors in Typical Steppe of Inner Mongolia under Long-term Nitrogen Addition

We investigated soil respiration (Rs) dynamics and influencing factors under different nitrogen (N) addition levels (0, 2, 4, 8, 16, 32 g m^-2 yr^-1) on typical grassland plots in Inner Mongolia. We measured soil respiration, temperature, moisture and nutrients. We found that N addition did not change dynamic characteristics of Rs; daily and seasonal dynamics followed a single peak curve. N addition reduced Rs during the growing season. Rs under N2, N4, N8, N16 and N32 treatments decreased by 24.00%, 21.93%, 23.49%, 30.78% and 28.20% in the growing season, respectively, compared to the N0 treatment. However, Rs in the non-growing season was not different across treatments. Rs was significantly positively correlated with soil temperature and moisture and these two factors accounted for 72%-97% and 74%-82% of variation in Rs, respectively. The soil respiration temperature sensitivity (Q₁₀) was between 2.27 and 4.16 and N addition reduced Q₁₀ except in the N8 treatment.     

基于高通量测序方法分析荣成月湖潮间带趋磁细菌多样性

荣成月湖是一个典型天然潟湖,潮间带沉积物中存在多种形态趋磁细菌。通过Roche454高通量测序平台,对沉积物样品(B_S)和磁收样品(B_M)进行16SrRNA基因高通量测序,认识荣成潮间带沉积物的细菌群落结构,并了解趋磁细菌多样性及系统进化地位。研究结果表明沉积物样品中主要的细菌类群为δ-变形菌纲,占总细菌数的26.4%,其次是γ-变形菌纲和α-变形菌纲;而磁收样品中细菌多样性和种类明显降低,以α-变形菌纲占绝对优势,相对比例达72.6%。在磁收样品和沉积物样品分别发现了1 612条和186条reads与趋磁细菌相关,分别占细菌总数的5.76%和0.85%,磁收样品中趋磁细菌数是沉积物样品的6.8倍。对两个样品中获得的趋磁细菌序列进行系统进化分析,发现这些序列多数属于变形菌门的α-变形菌纲,以趋磁球菌占绝对优势,少数属于δ-变形菌纲,与多细胞趋磁原核生物亲缘关系最近。海洋趋磁螺菌属仅在B_M样品中检出,趋磁弧菌属在B_M样品优势度高于B_S样品,而多细胞趋磁原核生物和趋磁螺菌属在B_S样品中优势度更高。通过分析样品间的差异OTUs,认为荣成潮间带沉积物中可能存在大量未知的趋磁细菌新类群。研究结果为下一步培养和开发趋磁细菌这一功能菌群,发现趋磁细菌新类群及趋磁细菌生态功能提供了基础资料。