Total Synthesis of Marine Cyclic Enol-Phosphotriester Salinipostin Compounds

Due to their structural diversity and variety of biological activities, marine natural products have been the subject of extensive study. These compounds, especially phospholipid polycyclic aromatic hydrocarbons, have a wide range of pharmacological applications, including embedded DNA and central nervous system, anti-tumor, anti-virus, anti-parasite, anti-bacterial, and antithrombotic effects. Unfortunately, the insufficient drug sources have limited the development of these compounds. In this study, we isolated salinpostin compounds from a fermentation solution of marine-derived Salinospora sp., which has a common bicyclic enol-phosphotriester core framework, as well as potent and selective antimalarial activities against P. falciparum with EC_(50)=50 nmolL~(-1). The chemical synthesis of these compounds in greater quantities is necessary for their use in bioactivity studies. Thus we explored a short route with high yields and mild reaction conditions, which can generate combinatorial libraries for drug discovery and lead optimization. We developed a new total synthesis method for six cyclic enol-phosphotriester salinipotin compounds and their diastereomers. For the total synthesis of cyclipostin P, we prepared cyclic enol-phosphotriester salinipostin compounds in 10 steps from a readily accessible starting material, 1,3-dihydroxyacetone, and obtained an overall yield of 1.29%. We fully characterized these compounds by proton nuclear magnetic resonance(~1H-NMR), carbon-13 NMR(~(13)C-NMR), and high-resolution mass spectrometry(HRMS) analyses, and found they coincide absolutely with the same compounds reported previously.     

重庆红池坝地电场变化分析

利用磁静日时序叠加方法和FFT频谱分析方法对红池坝地电场观测资料进行逐月处理,并与巫山建坪台地磁观测资料进行对比,分析红池坝地电场静日变化特征;计算红池坝台站磁暴期间产生的感应电场,与地电场观测数据对比,分析地电暴的特征。结果表明,静日随着月份的变化,相位发生变化,与巫山建坪地磁Sq变化一致;1月、2月、11月、12月的地电场日变幅明显小于其他月份;地电场显著周期成分与磁静日地磁场相同,并且通过周期成分的逐月对比分析得到,地电场与地磁D分量的不同周期成分的频谱值随时间的变化基本一致;某一方向的地电暴与该垂直方向的磁暴和该地区的电性结构有关;地电暴观测值与地磁感应电流计算值呈线性关系;地电暴变化与K值呈指数关系。     

峪耳崖金矿的成矿时代裂变径迹研究

通过峪耳崖金矿含矿岩体中的锆石、磷灰石裂变径迹分析，揭示了自中生代燕山期以来峪耳崖地区经历了频繁、多期次的热液活动，且与峪耳崖金矿的形成密切相关。锆石、磷灰石的裂变径迹年龄显示，金矿成矿时代主要为115～200Ma，成矿下限为82Ma。其中，115～190Ma是主要的成矿时期。成矿流体作用遍及整个岩体；流体的运移受到构造制约，具有NE—SW延伸的趋势；成矿流体具深部来源以及在地表水的参与下成矿。峪耳崖金矿的形成，与当时整个中国东部的动力学背景有关。     

海冰爬坡和堆积行为的试验研究

对于浅海区采油平台等设施常采用斜坡式人工岛形式,虽然在一定程度上起到降低海冰对平台的作用,但是海冰沿着斜坡上爬至平台并形成堆积,同样也会对平台设施造成破坏,因此有必要对海冰堆积爬坡的关键参数进行研究。通过在冰排前进方向放置混凝土实体结构物模拟海冰堆积和爬坡过程,分析了模型冰爬坡和下滑角度、最大堆积高度等关键参数以及模型冰断裂长度的统计特性及其与变形模量的关系,试验结果表明水位越高,冰排越容易发生堆积爬坡。破碎的模型冰在结构物前堆积后,形成爬坡角和下滑角。爬坡角随着堆积体的增大而增大,下滑角则逐渐减小。堆积冰高度一定时,高度不再增加,在来冰方向会形成新的堆积。这种现象为浅海区海上结构物的防冰减灾提供了新思路。     

The relationship between soft-bottom macrobenthic communities and environmental variables off Ningjin, eastern Shandong Peninsula

Macrobenthic infauna and associated environmental factors influencing the benthic community in the eastern coastal region of Shandong Peninsula were analyzed in four seasonal surveys from January 2007 to October 2007 (30 stations in winter, 20 stations in other three seasons), in order to understand the community structure and the factors unfluencing the benthic distribution. PRIMER 6.0 and SPSS 15.0 software packages were adopted to analyze the environmental and macrobenthic data. The results show that there were 260 macrobenthic species in total collected from the research region. The composition of species is:Polychaeta (94 species), Crustacea (75), Mollusca (56) and Echinoderm (12), among which, only 23 species were common species in the cruises of every season. The dominant species varied from season to season; however, the polychaete species Paralacydonia paradoxa Fauvel and Echinoderm species Amphioplus japonicus (Matsumoto) were always present year-round. The abundance and biomass of the macrobenthos in the research region were variable from season to season. The results of CLUSTER and MDS analysis show that the similarities of macrobenthic structures between the stations were low; most of the similarities were at about 30% of similarity value, only two stations were up to 70%. In accordance with the similarity values of the macrobenthic structures, we divided the 20 stations into five groups by arbitrary similarity level of 30%. The ABC curve indicates that the marcofauna communities in the research region had not been disturbed massively, except two stations, SB1 and SB3. Ecologically, benthos were controlled by a combination of factors such as salinity, phytoplankton, zooplankton, SiO₃-Si and temperature, and no single factor could be considered as an ecological master factor.     

日本鬼鲉不同组织的8 种同工酶谱

采用垂直聚丙烯酰胺平板电泳技术,对象山港野生日本鬼鲉(Inimicus japonicus)的9种组织器官的8种同工酶进行研究,并分析了其酶谱表型。结果表明,琥珀酸脱氢酶(Succinatedehydrogenase,简称SuDH)在所有组织中均存在,组织间差异较小;乳酸脱氢酶(Lactic dehydrogenase,简称LDH)、苹果酸脱氢酶((Malate dehydrogenase,简称MDH)、苹果酸酶(Malic enzyme,简称ME)、酯酶(Esterase,简称EST)、醇脱氢酶(Alcoholdehydrogenase,简称ADH)、过氧化物酶(Peroxidase,简称POD)、超氧化物歧化酶(Superoxide dismutase,简称SOD)则具有明显的组织特异性,且这些酶在表型、分布和活性上均表现出高度的组织特异性。     

真江蓠杭州湾海域栽培试验及生态因子对藻体生长的影响

在杭州湾上海金山海域进行了真江蓠(Gracilaria verrucosa)栽培试验并在实验室内研究了温度、盐度和光照强度对真江蓠生长的影响。结果表明,温度、盐度和光照强度对真江蓠的生长影响极显著(P0.01)。真江蓠生长适宜温度为20~30℃;适宜盐度为15~25;适宜光照强度在90μmol/(m2.s)左右。温度、盐度和光照强度对真江蓠生长存在显著的交互效应(P0.05),三者的最佳组合为温度25℃、盐度20和光照强度90μmol/(m2.s)。海区栽培实验表明真江蓠可在杭州湾海域正常生长,全年平均特定生长率(RSG)为9.42%/d。     

胶州湾海域营养状况研究

根据2000～2009年胶州湾海水监测资料,选用单项指标分析和富营养化指数法,对胶州湾海域以及主要河口区营养水平进行评价。结果表明,胶州湾水质较差,无机氮严重超标,N/P值为62.7,呈现高N低P状况,整个海域呈中度-重度富营养状态。主要河口海域无机氮(IN)严重超标,富营养化程度高,其中墨水河口为严重富营养,楼山河口为重度富营养。     

海平面上升对河北黄骅台风风暴潮漫滩影响的数值研究

基于河北省黄骅地区的风暴潮漫滩灾害风险评估模型,利用成熟的业务化台风风暴潮数值预报模式、100m分辨率的风暴潮漫滩数值模式,以及该地区的海平面上升和地面沉降结果数据,对台风登陆地点、路径、方向进行科学组合,利用各种组合参数条件进行数值计算,得到了995、985、975、965hPa四种台风强度下,海平面上升对风暴潮漫滩的影响。在相对海平面上升50cm和100cm情况下,四种台风强度的风暴潮平均最大漫滩面积分别增加了约35%和86%;台风强度中等,即985或975hPa时,风暴潮最大漫滩面积的增加更为显著。研究表明,海平面上升对该地区的漫滩影响非常明显,有效防范风暴潮灾害的同时,在气候变化的大背景下,应进一步研究和提出应对海平面上升的长效防治措施。     

青蛤POD组织差异及温度骤升和窒息胁迫对青蛤POD的影响

研究了青蛤肝胰腺、鳃瓣、斧足、外套膜组织中过氧化物酶(POD)组织差异以及温度骤升和窒息胁迫对青蛤POD酶活的影响。结果表明:水温16℃和温度骤升至32℃环境下青蛤POD酶活性均存在不同程度的组织差异性,且以肝胰腺中的POD活性最高,而斧足最低。水温16℃环境下青蛤的肝胰腺、斧足、外套膜和鳃瓣这4个组织之间的POD酶活力除外套膜和鳃瓣无显著差异外,其余均存在明显的组织差异(P0.05),其中肝胰腺的POD酶活最高1.68±0.08U-mg-1,其次为鳃瓣、外套膜,而斧足中的POD活力最低0.19±0.02U-mg-1。同时温度骤升胁迫对青蛤的POD也有显著的影响,在瞬时32℃高温下5h时肝胰腺的POD酶活达到最大值3.85±0.06U-mg-1,11h时出现第二峰值3.47±0.22U-mg-1,15h后POD值达到处理前水平并逐渐趋于稳定;在窒息环境胁迫下肝胰腺的POD酶活在15h内变化无显著变化,与对照组水平接近,15h-17h酶活显著升高,17h达到最大值2.98±0.54U-mg-1,之后又恢复到对照组水平。