Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc

Seamounts are subsurface mountains in the ocean. Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions. Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts. We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc, in the tropical western Pacific Ocean by using high-throughput sequencing. Thaumarchaeota was dominant in the sediments of all sample stations. Community diversity and species richness were greatest at stations near the top of the seamount, and lowest at the deepest station. One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea. In summary, depth has an important influence on archaeal community structure, and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount. This study provides a foundation for future research on Archaea in seamounts.     

Microbial diversity in Huguangyan Maar Lake of China revealed by high-throughput sequencing

Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method. We found significant differences between the microbial community compositions of the water and the sediment. The sediment samples contained more diverse Bacteria and Archaea than did the water samples. Actinobacteria, Betaproteobacteria, Cyanobacteria, and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria, Anaerolineae, Nitrospira, and Dehalococcoidia were the major bacterial groups in the sediment. As for Archaea, Woesearchaeota (DHVEG-6), unclassified Archaea, and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water, whereas the Miscellaneous Crenarchaeotic Group, Thermoplasmata, and Methanomicrobia were significantly more abundant in the sediment. Interactions between Bacteria and Archaea were common in both the water column and the sediment. The concentrations of major nutrients (NO^3-, PO4^3-, SiO3^2- and NH4^+) shaped the microbial population structures in the water. At the higher phylogenetic levels including phylum and class, many of the dominant groups were those that were also abundant in other lakes;however, novel microbial populations (unclassified) were often seen at the lower phylogenetic levels. Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs.     

GNSS精密轨道产品不连续性分析及评估

按照目前的国际规范, 高精度GNSS (Global Navigation Satellite System)轨道产品一般以天为周期进行发布, 提供给用户使用. 连续使用多天的产品存在不同天间的跳变问题. 利用德国地学研究中心(GFZ)、欧洲定轨中心(COD)、欧空局(ESA)、美国喷气试验室(JPL)以及上海天文台(SHA)共5个GNSS分析中心2013---2017年的轨道产品, 分析了轨道跳变的特性. 计算结果表明: GFZ、COD、ESA、SHA和JPL的3维轨道跳变平均分别为7.79cm、1.51cm、7.77cm、11.75cm和2.51cm. 轨道跳变序列的周期特性分析表明: 序列存在90d、120d、340d左右的显著周期项, 对应于海潮对地球自转的影响, 其振幅为数毫米至1cm左右. 表明精密轨道确定需要进一步精化该项模型; GPS的跳变序列还存在与卫星星座相关的175d和352d左右的交点年显著周期项. 此外, 针对COD产品外推轨道的分析, 验证了地球反照辐射压和太阳光压模型等动力学模型对轨道的差异.     

异源多分辨率卫星影像匹配算法研究

以异源多分辨率遥感卫星影像为研究对象,对影像特征提取、特征描述、特征匹配及匹配提纯算法进行了较为深入的研究;并将不同的特征提取与匹配算法进行组合,通过试验对比得出了各种算法的适用性。在此基础上,设计了一套面向异源多分辨率卫星影像的匹配流程。     

粒子加速器中高精度丝线绝对位置测量技术研究

粒子加速器中采用振动线技术进行共架单元上多块磁铁的高精度预准直,需要将振动线代表的磁中心引出进行共架单元之间的相对准直。为了获取以振动线为代表的丝线高精度空间绝对位置,本文对采用近景摄影测量技术进行点、线位置求解的方法进行了研究。利用单相机多站位的方法进行拍摄,通过标定板提供的高精度控制点坐标,采用自标定光束法平差对相机进行标定,并提出将空间直线离散成空间2点进行直线整体精确求解。对标定板上由2点组成的模拟直线进行测量验证,并与标定板提供的理论值进行对比,结果显示空间直线位置获取精度优于5 μm。这表明了本文理论和方法的正确性,为进一步研究丝线高精度空间绝对位置测量打下了基础。     

不规则三角网数字水深模型缓冲面快速构建的滚动球加速优化算法

针对TIN_DDM缓冲面构建与应用中存在的数据类型特殊、算法效率与模型精度不匹配的问题,本文将滚动球模型应用扩展至TIN_DDM缓冲面的构建过程。在分析滚动球模型构建精度局限的基础上,建立了滚动球半径关联的滚动球模型整体精度控制方法;结合大数据量TIN_DDM缓冲面多次构建的应用效率需求,阐明了关键采样点与滚动球半径对TIN_DDM缓冲面构建效率的影响规律;设计了TIN_DDM缓冲面构建关键采样点的判定准则,建立了关键采样点与滚动球半径的数值关联关系;提出了一种基于滚动球加速优化模型的TIN_DDM缓冲面快速构建算法,算法时间复杂度为O(n)。试验结果表明:本文算法可实现任意缓冲半径条件下TIN_DDM缓冲面的多次快速构建,且算法精度控制在2σ内。     

社区生活圈公共服务设施配置的空间分异分析——以大连市沙河口区为例

城市公共服务设施的配置水平直接影响到居民的生活品质。论文基于高德地图获取的地图数据,使用城市网络分析工具(Urban Network Analysis Toolbox, UNA)对大连市沙河口区内的6类公共服务设施分布进行空间分异分析,并对区内90个社区的居民出行可达设施的数量及类别进行测度,最后对各个社区进行总体评价。结果表明：① 大连市沙河口区公共服务设施空间分布不均,北多南少,呈现出“一主多次”的“廾”字形的结构,主要分布在西安路商业区周边和各个街道的次级商业区;② 居民可达设施数量空间差异大,北多南少,各街道均有1或2个区域可达设施数量较多;③ 居民可达设施类型数量存在一定差异,70%以上的住宅楼中的居民可以获得全部6类公共服务设施;④ 各社区居民可达设施总体处于差异较小的均衡状态,但仍有个别社区存在内部差异较大的现象。设施的分布、地物的阻隔和社区的封闭程度对居民获取各类公共服务设施影响较大,建议构建“设施完善、便捷可达”的15 min社区生活圈。     

Formation of Li Salts from Natural Brines during Isothermal Evaporation at 25℃

Li brines are the primary resources for Li salt industries. Evaporation is necessary to concentrate Li due to its low level of concentration in raw brines. The salt sequences during the evaporation of Li brines,especially the behavior of Li salts,represent key data for solar technologies. However,chemists cannot use any phase diagram to estimate Li salt sequences during evaporation at 25℃ . The thermodynamic model proposed by us in2003 represents the only tool for the prediction of equilibrium conditions during the evaporation of solutions containing Li~+,Na~+,K~+,Mg~(2+)/Cl~-,SO_4~(2-),and-H_2 O components at 25℃ . In this paper,the predicted salt sequences of 20 brines are reported. The results indicate that( 1) the first crystallized Li salt during evaporation of Li brine varies in brine composition;( 2) lithium sulfate is crystallized in many cases initially for brines of magnesium sulfate subtype,while Db4( Li_2 SO_4·K_2 SO_4) or Db3( 2 Li_2 SO_4·Na_2 SO_4·K_2 SO_4) appears first for sodium sulfate and magnesium sulfatesubtypes with lower Mg/Li composition,and the final eutectic point is H ~+ LiC ~+ Lc ~+ Ls ~+ Car;( 3) the final eutectic point is H ~+ LiC ~+ Lc ~+ Car for brines of chloride type; and( 4) Li content corresponding to the first crystallized Li salt is in the range of 0. 43%-1%. These findings enhance our knowledge of Li chemistry and provide insights into solar pond technology of the Li-brine process.     

基于电化学法的铬盐清洁生产工艺研究

现有铬盐生产技术阻碍了铬盐行业的清洁化发展,研发新型铬盐生产技术实属必要。中国科学院青海盐湖研究所自行研发出一套以电化学法为基础的铬盐清洁生产工艺,涵盖三条大工艺和四种母系铬盐产品。包括氢氧化钠溶液中电化学氧化铬铁制备铬酸钠联产三氧化二铬和三氧化二铁工艺,离子膜电解法从铬酸钠合成重铬酸钠工艺,以及离子膜电解法从重铬酸钠合成铬酸酐工艺。相比现有铬盐生产技术,该技术的优势主要体现在以下五点:温和化、去渣化、高值化、自动化和高效化。目前相关研发工作业已走出实验室,走过产业化中间试验,正向产业化示范线迈进。     

条斑紫菜酵母双杂交文库的构建及PyMAPK5互作蛋白的筛选

为进一步研究条斑紫菜促分裂原活化激酶家族PyMAPK5的下游互作蛋白,理解其生物学功能,本研究通过酵母双杂交的方法进行其相互作用蛋白的筛选。提取不同温度和失水逆境胁迫下的RNA,利用Invitrogen体系构建条斑紫菜酵母双杂交cDNA文库,其库容为1.44×107CFU,重组率为91.8%。以pGBKT7-PyMAPK5为诱饵蛋白载体,利用共转化方法,从文库中筛选得到26个与PyMAPK5互作的候选蛋白。候选蛋白集中在光系统II相关蛋白、捕光蛋白、微管蛋白、ATP酶、GTP结合蛋白及假设蛋白等。微管蛋白、捕光蛋白、光系统II蛋白一对一验证结果为阳性,表明在酵母体内存在互作。本研究为阐明条斑紫菜PyMAPK5与其互作蛋白的关系及解析PyMAPK5下游作用机制奠定了基础。