Cloning and expression of metallothionein mutant alpha-KKS-alpha in Anabaena sp. PCC 7120

The mouse metallothionein (mMT) mutant alpha-KKS-alpha has a higher capacity for binding heavy metals than wild type mMT. The mMT mutant alpha-KKS-alpha gene was placed under the control of the strong promoter PpbsA to generate the intermediate vector pRL-alpha-KKS-alpha. pRLalpha-KKS-alpha was then linked with the plasmid pDC-08 to construct shuttle expression vector pDC-alphaKKS-alpha. This expression vector was transformed into Anabaena sp. PCC 7120 using triparental conjugative transfer. After antibiotic selection (ampicillin and kanamycin), transgenic Anabaena was identified by PCR and Western blotting. The expression level of the mMT mutation alpha-KKS-alpha reached 7.4 mg/g dry cells weight, as detected by ELISA, and heavy metal resistance of the transgenic Anabaena was significantly improved.     

瞬变电磁法数据提取纯异常方法技术

在瞬变电磁法数据处理中,借鉴小波分析的"自适应性"和"数学显微镜性质"的特点,提出了特殊的TEM数据二维小波变换处理方法和流程,并编制了可视化处理软件,其核心是利用计算出的异常Lipschiz指数α的正负变化区分正常场和异常场,从而有效地从TEM数据中提取纯异常。选取某铜镍矿上实测的TEM资料进行了处理,获得了令人满意的效果。     

给711雷达增加高显方位指示电路

在雷达观测中,对各种回波进行照相是一项重要的基础工作。由于711雷达没有自动照相装置,所以照相程序比较麻烦。为了简化操作和使所获得的资料准确及时,我们进行了一些改革。例如,在显示器的面板上,安装有照明的钟表和透明有机玻璃板,使照相时的日期、时间,天线仰角、衰减分贝数等要素,都能拍摄在胶片上。从而免去了照相登记簿和回波照     

B-1型冰川测厚雷达试验装置

用无线电回波探测冰川厚度的方法在许多国家早已应用。1979年5月我国也首次使 用国产KDL-A型矿井地质雷达,在祁连山羊龙河冰川上获得了清晰的冰川底面回 波。之后,我所根据高山冷性冰川的特点,进行改制,做成了B-1型冰川测厚雷达 试验装置。     

Patterns of inorganic phosphate uptake in Cassiopea xamachana: a bioindicator species

Nutrient levels in the nearshore waters of the Florida Keys have increased over the past few decades concomitant with a decline in the health of Florida's reef system. Phosphorus is a particular concern in the Florida Keys as it may be the limiting nutrient in nearshore waters. We demonstrate that the upside-down jellyfish, Cassiopea xamachana, decreases its rate of phosphate uptake following exposure to elevated levels of dissolved inorganic phosphate. We also show that this subsequent suppression of uptake rates persists for some time following exposure to elevated phosphates. Using these attributes, we experimentally investigated the use of C. xamachana as a bioindicator for dissolved inorganic phosphates in seawater. Our results show that these animals reveal comparative differences in environmental phosphates despite traditional testing methods yielding no detectable phosphates. We propose that C. xamachana is a bioindicator useful for integrating relevant information about phosphate availability in low nutrient environments.     

基于振动台试验的抗滑桩加固斜坡桥基研究

基于工程实践中研究斜坡桥基抗震加固的需要,设计并完成前后排抗滑桩加固滑坡桥基的振动台模型试验。通过加载不同频率、加速度峰值的正弦波,分析振动时桥墩基桩、抗滑桩的受力变形规律,探讨滑坡破坏发展过程和动力响应特性。试验结果显示,前后排抗滑桩均应与桥基保持合理距离,有利于桥墩基础的受力变形;受桥梁上部结构的动力影响,桥墩基桩应变沿桩深衰减,衰减速度与土体抗力相关;当后排抗滑桩开裂后,桩身应变骤降,桩后土压力出现卸荷效应,但抗滑桩仍有潜在的承载能力,同时滑坡从稳定性最差的区域开始破坏,逐渐产生频段耦合效应,在后排抗滑桩达到承载极限前,频段耦合效应显著,达到承载极限后,卸荷效应显著。