东海三叶原甲藻的形态特征及其ITS序列分析

报道了首次分离于东海海域的三叶原甲藻(Prorocentrum triestinum Schiller)藻株号(LAMB100721),通过利用光学显微镜、荧光显微镜、扫描电镜及分子生物学方法,对其形态特征、显微结构和分子系统进化进行了详尽描述和鉴定。细胞长卵形或披针形,后端细长且尖,前端圆,最宽部位于细胞中央。顶刺长而显,三角状。壳面光滑,无刺或突起物。刺丝胞孔稀疏而不规则地分布于壳面边缘。叶绿体无具体形状,分布于整个细胞中,细胞核球形,位于中下部。老化的细胞可见细胞边缘的间接带,且间接带表面光滑。细胞长为19~25 μm,平均值为(22.6±0.9)μm;宽为11~16 μm,平均值为(13.2±1.1) μm。所测目标藻株的rDNA ITS序列长度为569 bp,其中GC含量为47.6%。三叶原甲藻系赤潮种,为东海原甲藻春季大规模赤潮的伴随种。加强有害赤潮的预防和监测工作是减少危害的有效途径,而对赤潮原因种的准确识别和鉴定则是基础和关键。     

南海南部次表层叶绿素a质量浓度最大值及其影响因子*

文章分析了2013年南海南部4个季节航次的叶绿素a (Chl a)调查数据, 结果显示: 150m以浅水柱Chl a质量浓度均值分别为早春0.14mg•m^-3、初夏0.12mg•m^-3、初秋0.18mg•m^-3、初冬0.16mg•m^-3。早春和初夏偏低的原因与早春风速小, 初夏水温高, 不利于水体的垂直混合, 限制了深层海水中丰富的营养盐向上层水体补充有关。4个季节中海水次表层Chl a质量浓度最大值层(SCML)均出现在50m和75m, 这两个水层的Chl a质量浓度差异小, 季节变化不大, 平均值变化范围分别为0.24~0.26mg•m^-3和0.22~0.26mg•m^-3。受混合层深度和温跃层上界深度的共同影响, 50m水层Chl a质量浓度主要受制于深层富营养盐海水的向上补充, 75m水层Chl a质量浓度受水温的影响明显。     

便携式高频地波雷达海流探测长周期工作性能试验

2013年1月29日至3月15日在台湾海峡西南部海域进行了OSMAR-S100便携式高频地波雷达与浮标观测海流数据的长周期对比试验,验证了雷达系统在探测海流方面的准确性、可靠性和稳定性.通过实测海流与雷达矢量流的复相关分析,选定3 m层的海流为对比代表层.试验期间实测流速为0.0~120.0 cm/s,雷达海流有效探测区内的矢量流流速、流向的观测误差较小,能够满足实时监测海洋表层流的需要,高精度区流速、流向的均方根误差分别为9.1 cm/s和24.8°,边缘区的均方根误差为13.3~24.8 cm/s和39.4°~39.6°,与国内外达到业务化运行要求的同类产品实际观测精度相当.     

Heterosis and heritability estimates for the survival of the Pacific white shrimp (Litopenaeus vannamei) under the commercial scale ponds

The aim of the present study is to detect the potential of the base population from diallel crosses of eight introduced strains of the Pacific white shrimp(Litopenaeus vannamei) for improving the yield. Heterosis and heritability were estimated for pond survival at commercial farm conditions for the base population that included 207 full-sib families from a nested mating design by artificial insemination. Among all the hybrids,the heterosis ranged from –11.37%(UA1×UA2) to 20.53%(UA3×SIN) with an average of 0.953%. The results showed that more than half of the hybrids(51.85%) have negative heterosis for survival rate, but most of the hybrids with positive heterosis have high estimates. The high proportion of negative heterosis for survival rate reminders us that the survival trait also should be considered in the crossbreeding program to avoid yield decrease. However, high positive heterosis manifested in most of the hybrids for survival indicates the usefulness of these hybrids for improving the survival to obtain higher yield by crossbreeding in this breeding program. The heritability estimate for pond survival was 0.092±0.043 when genetic groups were included in the pedigree, and it was significantly different from zero(P0.05). The results from this study also indicated that significant improvement for survival is possible through selection in L. vannamei.     

利用模板阴影体算法实现矢量数据在三维场景中的绘制

提出了一种基于模板阴影体算法的矢量数据绘制方法,实现了矢量数据在三维场景中的高质量实时叠加显示。由于该方法基于屏幕空间,所以具有像素级的精度,不会出现传统的基于纹理方法所产生的绘制走样现象;而且不受地形几何数据的约束,其执行效率与地形数据的复杂度无关,仅取决于矢量数据本身的复杂度。详细论述了基于模板阴影体算法矢量数据绘制的关键技术,并通过试验验证了该方法的有效性。     

基于小波变换和HIS变换的海冰SAR与光学遥感影像融合方法研究

Sea ice as a disaster has recently attracted a great deal of attention in China. Its monitoring has become a routine task for the maritime sector. Remote sensing, which depends mainly on SAR and optical sensors, has become the primary means for sea-ice research. Optical images contain abundant sea-ice multi-spectral information, whereas SAR images contain rich sea-ice texture information. If the characteristic advantages of SAR and optical images could be combined for sea-ice study, the ability of sea-ice monitoring would be improved. In this study, in accordance with the characteristics of sea-ice SAR and optical images, the transformation and fusion methods for these images were chosen. Also, a fusion method of optical and SAR images was proposed in order to improve sea-ice identification. Texture information can play an important role in sea-ice classification. Haar wavelet transformation was found to be suitable for the sea-ice SAR images, and the texture information of the sea-ice SAR image from Advanced Synthetic Aperture Radar(ASAR) loaded on ENVISAT was documented. The results of our studies showed that, the optical images in the hue-intensity-saturation(HIS) space could reflect the spectral characteristics of the sea-ice types more efficiently than in the red-green-blue(RGB) space, and the optical image from the China-Brazil Earth Resources Satellite(CBERS-02B) was transferred from the RGB space to the HIS space. The principal component analysis(PCA) method could potentially contain the maximum information of the sea-ice images by fusing the HIS and texture images. The fusion image was obtained by a PCA method, which included the advantages of both the sea-ice SAR image and the optical image. To validate the fusion method, three methods were used to evaluate the fused image, i.e., objective, subjective, and comprehensive evaluations. It was concluded that the fusion method proposed could improve the ability of image interpretation and sea-ice identification.     

基于稳定同位素的东太平洋大青鲨(Prionace glauca)营养级研究

碳、氮稳定同位素为分析淡水和海洋生态系统的生物营养关系提供了有力手段,但在大洋鱼类中的应用还很少。本研究以热带东太平洋大青鲨(Prionace glauca)整节脊椎骨作为样品(叉长范围153—242cm),运用稳定同位素技术,分析大青鲨的δ~(13)C值和δ~(15)N值及其变化,比较不同基线生物的选择对营养级计算的影响。结果表明,大青鲨δ~(13)C范围为–15.76‰—–13.41‰,最大差值2.35‰;δ~(15)N范围为10.62‰—17.72‰,最大差值7.1‰;δ~(13)C值和δ~(15)N值随个体长度的变化不明显(可能原因是样品鱼均为较大个体),性别间的差异不显著,但不同基线生物的选择对营养级计算值的影响较大。本文研究表明,通过整节脊椎骨的稳定同位素测定来获得大青鲨的摄食特征信息是可行的。由于基线生物对营养级计算的影响问题难以在短期内解决,今后可将相对营养级或营养级的变化作为重点,研究人类活动(如捕捞)对大洋鱼类摄食和营养级的影响。     

诏安湾及毗邻海域浮游动物的群落结构

根据2015年夏(8月)、秋(10月)2季在诏安湾及毗邻海域开展的30个站浮游动物监测资料,研究了该海域浮游动物的种类组成、数量分布和多样性.结果显示:共鉴定浮游动物103种和浮游幼体24类,桡足类为绝对优势的类群.优势种有14种,锥形宽水蚤(Temora turbinata)、异体住囊虫(Oikopleura dioica)和强额孔雀水蚤(Parvocalanus crassirostris)是该海域重要的优势种群.生态类群以近岸暖水类群和外海广布类群为主.夏季浮游动物丰度平均为761个/m3,秋季为81个/m3;夏季浮游动物生物量平均为237mg/m3,秋季为52 mg/m3.夏季浮游动物种类数、丰度、生物量及变化幅度均明显高于秋季,夏季湾外浮游动物种类数、丰度、生物量及变化幅度均明显大于湾内,秋季湾外浮游动物种类数、丰度及变化幅度均明显大于湾内,夏、秋季浮游动物种类数和丰度总体分布均呈现由湾内往湾外逐渐增加趋势.夏季浮游动物多样性指数平均为2.46,秋季为3.14.与历史资料比较:本次调查海域浮游动物种类数和平均丰度均有较大幅度升高,但平均生物量有较大幅度下降.     

深圳湾福田红树林区藻类、纤毛虫等生物群落及其与环境的关系

对福田国家级红树林自然保护区近岸水域进行水质及生态调查,结果显示,深圳湾潮汐对红树林中微型生物生态及湿地污染状况产生了最为直接的影响;藻类在红树林中具有较好的水质污染指示作用,其生态功能大于纤毛虫、轮虫等微型生态类群;微型生物可通过食物链对污染物起放大和传递作用,传递途径在水体中以藻→纤毛虫为主,底质中以藻→底栖动物幼虫为主。     

中国鲎基因组微卫星富集文库的构建及分析

本研究旨在从DNA分子水平上研究中国鲎(Tachypleus tridentatus)种群遗传多样性及种群结构等相关信息,以期为保护其野生群体种质资源提供科学依据。本研究通过生物素—磁珠富集法筛选微卫星标记,构建中国鲎基因组微卫星文库。基因组DNA经Fast Digest Tru1I酶切后,选取400 bp~1000 bp片段,用生物素标记的(GT)15、(CT)15混合探针与其杂交,杂交复合物与链霉亲和素磁珠结合,捕获含有重复序列的微卫星片段,纯化后连接PMD19-T载体克隆,构建基因组微卫星文库。从334个阳性克隆中随机选取196个片段大于400 bp的阳性克隆进行测序,共获得127个微卫星序列,其中完美型占69.3%、非完美型占11.0%、复合型占19.7%。除探针使用的GT和CT的重复序列外,还筛选到多碱基GCT、TGG、AAAC、ACAA、GATTT、TTTTA的重复序列。根据微卫星序列设计选择合成40对引物,结果显示其中3对具有较高多态性,可作为进一步评价中国鲎野生种质资源等遗传信息的有效遗传标记。