校园网IP地址绑定系统的研究与设计

IP地址绑定和IP用户管理是校园网管理员的主要工作,作者介绍哈尔滨工业大学校园网IP地址绑定和IP用户管理系统的设计思路及实现技术,该系统是采用J2EE技术和Struts+Hibernate框架实现的B/S结构WEB应用系统,用于校园网用户的IP地址分配I、P地址绑定及IP用户管理等方面。该系统的实现与应用大大提高了网络管理员的工作效率、避免了操作失误、改善了对用户的服务质量。     

Nautilus矿业公司SMS勘探中采用的取样技术与装备

海底大块状硫化物(SMS)富含铜、锌、铅等金属,特别是富含贵金属金、银,引起了国际采矿业的浓厚兴趣。近年来,以澳大利亚Nautilus矿业公司为先驱代表的国际大型资源开发商和投资商已介入海底多金属硫化物的商业化开采。文中介绍了Nautilus公司在巴布亚新几内亚专属经济区针对海底块状硫化物矿床开展的一系列以商业开采为目的的勘探取样活动,详细介绍了勘探中所采用的基本方法与技术,并重点对其地质调查中所应用的拖网挖掘取样、ROV海底表面取样、大洋钻探船岩芯取样和专门开发的ROV岩芯钻探取样的装备、取样结果及作业效率进行了较为系统全面地介绍。     

Dynamic simulation of tropical coral reef ecosystem being disturbed by multiple situations

In consideration of the rapid degradation of coral reef ecosystems, the establishment of models is helpful to comprehend the degradation mechanism of coral reef ecosystems and predict the development process of coral reef communities. According to the characteristics of complex ecosystem of tropical coral reefs in China, the coral reef functional group is the core level variable; combined with the multiple feedback effects of coral reef functional groups and environmental changes, the study presents a coral reef ecosystem dynamics model with hermatypic corals as the core. Based on the simulation of the assumed initial value and the internal feedback of the system, the results show that in the basic simulation(relative health conditions), the coverage area of live corals and coral reefs generally decreased first and then increased, and increased by 4.67% and 6.38% between2010 and 2050, respectively. Based on the calibration model and the current situation of the studied area, the multi-factor disturbance effects of coral reef communities were simulated and explored by setting up three scenarios involving fishing policy, terrestrial deposition, and inorganic nitrogen emissions. Among them, in the single factor disturbance, the fishing policy exerts the most direct impact on the community decline; and the succession phenomenon is obvious; the terrestrial sedimentation has a faster and more integrated effect on the community decline; the effect of inorganic nitrogen emission on the community decline is relatively slow. In the double/multi-factor disturbance, the superimposed disturbance will aggravate the multi-source feedback effect of the coral reef communities development, accelerate the community decay rate, and make its development trajectory more complicated and diverse. This method provides a scientific and feasible method for simulating the damage of long-term coral reef community and exploring the development law and adaptive management of coral reef ecosystems. In the future, it can be further studied in the ecological restoration process and decisionmaking direction of coral reefs.     

Impacts of anthropogenic activities on the Changjiang(Yangtze)estuarine ecosystem(1998–2012)

Estuarine ecosystem has greatly changed in the recent decades due to anthropogenic perturbations in the Changjiang Estuary. Change patterns and impact factors were analyzed based on the continuous data...     

温度突变对大海马(Hippocampus kuda)幼体生长、组分及酶活力的影响

以大海马幼体为实验材料,通过设置不同的温度突变组(温度从23℃突变至15℃、28℃和33℃)的方法,对其生长、生化组分以及酶活力的影响进行了研究。结果表明,实验结束后,28℃温度组的大海马幼体生长指标、蛋白含量、能值显著高于23℃对照组(P<0.05),而15℃、33℃温度组的各项指标则显著低于对照组(P<0.05);此外,温度突变组的大海马幼体的酶活力均有先升后降的趋势,在1d后出现峰值,4—6d各个温度组趋于稳定,到实验第15天时,28℃温度组的SOD、ACP活力和MDA的含量已处于23℃对照组水平(P>0.05),CAT、AKP活力显著高于23℃对照组(P<0.05)。而15℃、33℃温度组的SOD、CAT活力降至低于23℃对照组水平(P<0.05),15℃温度组的ACP、AKP活力则低于23℃对照组水平(P<0.05),MDA的含量在15℃、33℃温度组随时间延长而增加。     

Separation and relationship of ten marine scuticociliates (Protozoa, Ciliophora) using RAPD fingerprinting method

On the basis of the random amplified polymorphic DNA-fingerprinting (RAPD) method, 10 morphospecies of scuticociliates from 7 genera, including 15 clones of 13 strains, Uronema marinum, Uronema cf marinum, Parauronema virginianum, P. longum, Metanophrys similis, M. sinensis, Paralembus digitiformis, Mesanophrys carcini, Paranophrys magna and Cohnilernbus verrninus were analysed using 8 oligonucleotide primers. The genetic similarity among the clones of the same strain measured by a band-sharing index is 0.97～0.98,while 0.40-4).52 among strains. This value measured is 0.39～0.46 among congeners of the same genus, whereas 0.16～0.47 between different genera. A distance tree was constructed based on 8-primer analysis, in which the scuticociliates investigated were separated into two clusters: one consists of 2 genera, Uronema and Parauronema, and the other was composed of five, Metanophrys, Mestmophrys,Paranophrys, Paralembus and Cohnilembus. It is demonstrated also that the morphospecies Parauronema virginianurn may be a species-complex, i. e., it contains different genospecies.     

正交实验法优化紫蛇尾皂苷提取工艺研究

以紫蛇尾(Ophiopholis mirabilis)皂苷溶液吸光度为指标,采用单因素实验法考察乙醇体积分数、料液比、提取时间、提取次数、提取温度等因素对紫蛇尾皂苷提取效果的影响,通过正交实验确定最佳工艺条件。结果表明,紫蛇尾皂苷的最佳提取工艺为60℃水浴,料液比1:40(W/V),每次3 h,提取4次。按照最佳工艺条件所得蛇尾皂苷平均产率为1.77‰,表明此最佳工艺可用于紫蛇尾皂苷的制备。     

温度对WSSV感染中国对虾越冬亲虾的影响

在不同温度下,对中国对虾(Fenneropenaeus chinensis)越冬亲虾进行人工感染白斑综合症病毒(WSSV)实验,利用腹部采血和剪取游泳足进行活体取样.统计感染对虾发病及死亡情况,利用巢式PCR技术检测WSSV,统计实对虾受WSSV感染情况.结果显示,在对虾累计死亡率上游泳足取样的高温组(18～21 ℃)和低温组(9～12 ℃)有显著差异(P=0.041<0.05),高温组明显高于低温组;在对虾受WSSV感染程度上,高温组主要呈严重感染(91.67%),中温组(13～16 ℃)和低温组则主要呈轻度感染,且温度越低受感染程度越轻;在跟踪WSSV感染对虾进程研究上,高温组在48 h检测出WSSV,中温组和低温组则分别在72 h和96 h检测出WSSV,明显滞后于高温组.表明温度对WSSV感染对虾有一定的影响,低温(9～12 ℃)条件下WSSV也能感染对虾且导致死亡,但低温可抑制WSSV的增殖,减缓感染进程,延迟白斑综合症(WSS)的发生.     

青藏高原冬小麦田辐射能量收支的初步研究

通过对青藏高原冬小麦田净全辐射各分量的观测资料分析，论述了净全辐射及其各分量的日变化特征；计算得出冬小麦抽穗—乳熟期麦田平均反射率为13.3%，净全辐射占总辐射百分率:白天75%，包括夜间67.4%；指出了净全辐射和总辐射间存在良好的线性关系，给出了由总辐射计算净全辐射的经验公式。