How tides and river flows determine estuarine bathymetries

For strongly tidal, funnel-shaped estuaries, we examine how tides and river flows determine size and shape. We also consider how long it takes for bathymetric adjustment, both to determine whether present-day bathymetry reflects prevailing forcing and how rapidly changes might occur under future forcing scenarios.Starting with the assumption of a 'synchronous' estuary (i.e., where the sea surface slope resulting from the axial gradient in phase of tidal elevation significantly exceeds the gradient in tidal amplitude ), an expression is derived for the slope of the sea bed. Thence, by integration we derive expressions for the axial depth profile and estuarine length, L, as a function of and D, the prescribed depth at the mouth. Calculated values of L are broadly consistent with observations. The synchronous estuary approach enables a number of dynamical parameters to be directly calculated and conveniently illustrated as functions of and D, namely: current amplitude Û, ratio of friction to inertia terms, estuarine length, stratification, saline intrusion length, flushing time, mean suspended sediment concentration and sediment in-fill times.Four separate derivations for the length of saline intrusion, L_I, all indicate a dependency on (U_o is the residual river flow velocity and f is the bed friction coefficient). Likely bathymetries for `mixed' estuaries can be delineated by mapping, against and D, the conditions L<sub>I</sub>/L<1,E<sub>X</sub>/L<1 (E<sub>X</sub> is the tidal excursion) alongside the Simpson-Hunter criteria D/U<sup>3</sup><50 m<sup>−2</sup> s<sup>3</sup>. This zone encompasses 24 out of 25 `randomly' selected UK estuaries.However, the length of saline intrusion in a funnel-shaped estuary is also sensitive to axial location. Observations suggest that this location corresponds to a minimum in landward intrusion of salt. By combining the derived expressions for L and L_I with this latter criterion, an expression is derived relating D_i, the depth at the centre of the intrusion, to the corresponding value of U_o. This expression indicates U_o is always close to 1 cm s⁻¹, as commonly observed. Converting from U_o to river flow, Q, provides a morphological expression linking estuarine depth to Q (with a small dependence on side slope gradients).These dynamical solutions are coupled with further generalised theory related to depth and time-mean, suspended sediment concentrations (as functions of and D). Then, by assuming the transport of fine marine sediments approximates that of a dissolved tracer, the rate of estuarine supply can be determined by combining these derived mean concentrations with estimates of flushing time, F_T, based on L_I. By further assuming that all such sediments are deposited, minimum times for these deposition rates to in-fill estuaries are determined. These times range from a decade for the shortest, shallowest estuaries to upwards of millennia in longer, deeper estuaries with smaller tidal ranges.     

Inhomogeneous substrate analysis using EM300 backscatter imagery

Backscatter reflectivity from multibeam echo-sounders provides a powerful tool to efficiently characterize seafloor substrates. A comprehensive EM300 bathymetric and backscatter survey has been completed of Cook Strait, in central New Zealand. This paper presents a detailed analysis of the realtime corrections applied to the raw EM300 multibeam data and additional corrections required to compute angular variations of the backscatter strength. The corrections, including the local absorption coefficient, the influence of seafloor topography and sound refraction in the water column, are determined for different Cook Strait seafloor substrates. Modifying MB-System software code, we extracted the backscatter signal parameters in order to quantify the raw backscatter strength and apply additional processing. Profiles of backscatter strength versus incidence angle were computed for a variety of sites characterized by flat seafloor and homogeneous substrates, and for which ground-truth data were available. For each homogeneous site, different but characteristic backscatter profiles are observed that can be interpreted in terms of sediment facies. To analyze heterogeneous substrates, we present a statistical technique, based on a 3-dimensional distribution of (incidence angle, backscatter strength) couples that preserves geological information of the substrate components. This analysis, using backscatter data acquired on a submarine volcano, north of New Zealand, clearly differentiates soft sediments and lava flows within a heterogeneous substrate.     

Management of a shallow temperate estuary to control eutrophication: The effect of hydrodynamics on the system’s nutrient loading

The Mondego estuary, a shallow warm-temperate intertidal system located on the west coast of Portugal, has for some decades been under severe ecological stress, mainly caused by eutrophication. Water circulation in this system was, until 1998, mainly dependent on tides and on the freshwater input of a small tributary artificially controlled by a sluice. After 1998, the sluice opening was effectively minimised to reduce the nutrient loading, and the system hydrodynamics improved due to engineering work in the upstream areas. The objective of the present study was to evaluate the effect of the mitigation measures implemented in 1998. Changes to the hydrodynamics of the system were assessed using precipitation and salinity data in relation to the concentrations of dissolved inorganic nutrients, as well as the linkage between dissolved N:P ratios and the biological parameters (phytoplankton chlorophyll a concentrations, green macroalgal biomass and seagrass biomass). Two distinctive periods were compared, over a ten year period: from January 1993 to January 1997 and from January 1999 until January 2003. The effective reduction in the dissolved N:P atomic ratio from 37.7 to 13.2 after 1998 is a result of lowered ammonia, but not the oxidised forms of nitrogen (nitrate plus nitrite), or increased concentrations of dissolved inorganic phosphorus. Results suggest that the phytoplankton is not nutrient limited, yet maximum and mean biomass of green macroalgae was reduced by one order of magnitude after the mitigation measures. This suggests that besides lowering the water residence time of the system, macroalgal growth became nitrogen limited. In parallel to these changes the seagrass-covered area and biomass of Zostera noltii showed signs of recovery.     

Glucuronidation in the polar bear (Ursus maritimus)

Polar bears bioaccumulate lipophilic pollutants, including polychlorinated biphenyls (PCBs), into their bodies from their exclusive diet of marine organisms. Hydroxylated PCB metabolites (OH-PCBs) have been found in plasma, presumably due to CYP-dependent biotransformation of PCBs in liver. Little is known about the phase 2 metabolism of hydroxylated xenobiotics in polar bears. The objective of this study was to examine UDP-glucuronosyltransferase (UGT) activity with OH-PCBs and a hydroxylated polycyclic aromatic hydrocarbon, 3-hydroxy-benzo(a)pyrene (3-OH-BaP), in polar bear liver. Samples of frozen polar bear liver were used to prepare microsomes. UGT activity with 3-OH-BaP in Brij-treated microsomes, measured by a fluorescence assay, was readily measurable with protein concentrations in assay tubes of up to 10 g/ml, but dropped off very sharply at higher protein concentrations. The apparent Km for 3-OH-BaP was 1.71 +/- 0.04 microM, and Vmax 1.26 +/- 0.16 nmol/min/mg protein (mean +/- SD, n=3). UGT activities with a model tetrachloro-OH-PCB (4'-OH-CB72) and a model hexachloro-OH-PCB (4'-OH-CB159) were assayed with [14-C]-UDPGA and separation of the [14-C]-glucuronide by ion-pair extraction and thin-layer chromatography. [14-C]-glucuronide conjugates were readily formed by polar bear liver microsomes in the absence of added substrate, apparently from contaminants present in liver. This phenomenon was not observed using hepatic microsomes from laboratory-held catfish. Glucuronidation efficiency was much higher with 4'-OH-CB72 (Km 7.3 microM; Vmax 1.55 nmol/min/mg) than 4'-OH-CB159 (Km 16.1 microM; Vmax 0.46 nmol/min/mg). The identities of the aglycones present in polar bear liver are not known, but could include OH-PCBs or hydroxylated metabolites of other persistent organic pollutants. This study demonstrates that UGT with high activity for 3-OH-BaP and other substrates is present in polar bear liver.     

The Dynamics of Covering Behaviour in Dominant Echinoid Populations from American and European West Coasts

Abstract.  The so-called 'covering behaviour' of echinoids is known to vary with habitat according to the types of covering items available, but the full extent of the role played by habitat characteristics in this behaviour is not known. Moreover, whether or not different species inhabiting similar environmental conditions and habitats also show similar patterns of this particular behaviour has yet to be investigated. In this study, two prominent west coast echinoid species, Paracentrotus lividus and Strongylocentrotus purpuratus , were examined with respect to covering behaviour and spatial organisation. Populations of both species are found in exposed intertidal zones, occupying boreholes within tidepools. Important similarities were found in the spatial organisation of both species' populations, with lowest densities in upper shore pools. However, the size distributions of the two populations differed significantly; P. lividus within upper shore pools were significantly larger than those in mid or lower shore pools, whereas S. purpuratus on the lower shore were significantly larger than those at other shore heights. We found significant between-species variability in covering item use, although the number of covering items available at both sites was not significantly different. For example, greater densities of covering items were used by P. lividus than by S. purpuratus . We also found that the percent of echinoid surface area covered varied significantly in space for both species. These data emphasise the complex and multifaceted nature of covering behaviour, but most importantly, they also strongly suggest that habitat characteristics can only partly explain the extent of this behavioural variability amongst echinoid species.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of ≈49 kDa size, and weak signals for proteins of ≈41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of ≈56 and 59 kDa size, and also a weak immunoreaction with a protein of ≈43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14–40 ng/g DW in controls to 373–638 ng/g DW in exposed mussels.     

Simulating a heavy metal spill under estuarine conditions: Effects on the clam Scrobicularia plana

We describe the effect of heavy metals Zn, Cd, Pb and Cu on the induction of methallothioneins on the clam Scrobicularia plana along a salinity gradient simulated under laboratory conditions. The clams were exposed to constant heavy metal concentrations in a dynamic estuary simulator during a 15-day assay to investigate possible induction of metal-binding proteins in them. The concentration of heavy metals in water was analysed. Clams were analysed for methallothionein concentrations. The speciation of Zn, Cd, Pb and Cu along the salinity gradient was modelled. Zn showed the highest concentrations and its prevalent species was the free ion. Intersite differences have been observed in methallothionein concentration and related to the salinity gradient. It seems that synthesis of methallothioneins is the result of physiological forces acting in concert with the changes in the chemical speciation of metals, owing to the trace metals uptake is controlled by means of an interaction of physiology and physicochemistry.     

The optimum control of thruster system for dynamically positioned vessels

An offshore vessel with a dynamic positioning system (DP system) needs fast response to produce thrust to counteract the environmental forces acting on it for the purpose of maintaining its position and heading as close as possible to the working position. Therefore, quick and effective modulation of the thrust is the problem to determine the thrust and the rotation angle of the thruster devices of the ship. This paper presents an effective optimum control for a thruster system, using the sequential quadratic method to achieve economical and effective modulation of the thrust and the direction of the thruster. An optimum control study of a 2280 tons DP coring vessel with five rotary azimuth thruster marine positioning is studied in detail, which can quickly and exactly estimate the thrusts and angles of direction of all the thrusters. The results can provide a valuable thruster system for a dynamically positioned vessel.