BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.     

生物生态防治虾病研究

本文探索虾病防治新途径,采用低值海藻江蓠和对虾混养的生态学方法,江蓠吸收水中二氧化碳进行光合作用,产生大量氧气;同时吸收有机物的分解物 NH_3—N,加速有机物分解。减少 H_2S 积累;从而消除水中紧迫因子威胁,大大减少池水自污及病害,起到抑菌、抑虫、净化水质作用,提高成活率,试验圹对虾成活率达95.1%,对照圹56.6%。试验结果表明,虾病防治效果显著,经济效益明显。     

Methane hydrate formation comparisons in media with and without a water source supply

Water is a necessary element during gas hydrate formations. Therefore, by analyzing water depletion changes in media, the reaction characteristics of methane hydrate in media can be studied. In this study, two water sources supplying some liquid water which may be consumed by the methane hydrate formation reactions were designed and assembled. Using them, the full formation processes of methane hydrate was studied. Experimental results show the following： If heat released from nucleation reaction of methane hydrate is diffused rapidly, the nucleation ratios will be enhanced discernibly. While the hydrate is formed, a force is generated that sucks fresh water from the source into the vicinity of the hydrate, slowing down the cementation process and causing some hydrate grain dissociation. As a result of cementation differences, the hydrate reaction processes with different water sources present linear or quadratic equation characteristics. After a few repeated dissociation and formation processes of some hydrate grains caused by the fresh water, the gas amounts contained in hydrate will be significantly enhanced.     

Damming of large river by debris flow: Dynamic process and particle composition

The frequency and extent of debris flows have increased tremendously due to the extreme weather and the Wenchuan earthquake on May 12, 2008. Previous studies focused on the debris flow from gullies damming the mountain streams. In this paper, an equation for the run-out distance of debris flow in the main river is proposed based on the dynamic equation of debris flow at different slopes given by Takahashi. By undertaking field investigations and flume experiments, a new calculation method of the volume of debris flow damming large river is obtained. Using the percolation theory and the renormalization group theory it was deduced that the large particles should comprise more than 50% for forming a stable debris flow dam. Hence, the criteria of damming large river by debris flow is presented in terms of run-out distance and grain composition which was then validated through the event of damming river by debris flow at Gaojia gully, the upper reaches of the Minjiang River, Sichuan, China, on July 3, 2011.     

Impact factors identification of spatial heterogeneity of herbaceous plant diversity on five southern islands of Miaodao Archipelago in North China

Herbaceous plants are widely distributed on islands and where they exhibit spatial heterogeneity. Accurately identifying the impact factors that drive spatial heterogeneity can reveal typical island biodiversity patterns. Five southern islands in the Miaodao Archipelago, North China were studied herein. The spatial distribution of herbaceous plant diversity on these islands was analyzed, and the impact factors and their degree of impact on spatial heterogeneity were identified using CCA ordination and ANOVA. The results reveal 114 herbaceous plant species, belonging to 94 genera from 34 families in the 50 plots sampled. The total species numbers on different islands were significantly positively correlated with island area, and the average α diversity was correlated with human activities, while the β diversity among islands was more affected by island area than mutual distances. Spatial heterogeneity within islands indicated that the diversities were generally high in areas with higher altitude, slope, total nitrogen, total carbon, and canopy density, and lower moisture content, pH, total phosphorus, total potassium, and aspect. Among the environmental factors, pH, canopy density, total K, total P, moisture content, altitude, and slope had significant gross effects, but only canopy density exhibited a significant net effect. Terrain affected diversity by restricting plantation, plantation in turn influenced soil properties and the two together affected diversity. Therefore, plantation was ultimately the fundamental driving factor for spatial heterogeneity in herbaceous plant diversity on the five islands.     

基于STSM的入境旅游流集散地域结构特征分析——以中国入境旅游六大典型区域为例

构建入境旅游流空间转移态矩阵模型,基于入境旅游统计数据与抽样调查数据,在合理划定中国入境旅游典型区域的基础上,实证研究六大典型区域的入境旅游流空间转移态矩阵,探讨入境旅游流集散的地域结构特征。研究表明:入境旅游流在中国东部表现为"等级式"集散模式,在中国西部表现为"等级式"与"接触式"并存的集散模式;入境旅游流集散的地域空间集中性十分突出,且集聚源区域与扩散目的地区域呈现高度重合性,尤其六大典型区域之间的互动关联极其显著;中国入境旅游流集散地域空间的梯级差异化特征鲜明,整体呈现"东—西递进式"的发展格局。     

Tectonic-Thermal Evolution History and Its Controls on Petroleum Geology of Weibei Uplift

<正>1 Introduction The Weibei Uplift is located in the southwest of the North China Plate,where is the stable block(the Ordos Block)in the north and the active belt(the QinlingOrogenic Belt)in the south(Ren et al,2014,2015).And the belt is separated from the Weihe basin.The Weibei uplift has a uniform crystalline basement with the North     

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低高度角大气静力学延迟修正的改进

进行低高度角观测是GPS(Global Positioning System)气象学的发展趋势,其中发展高精度的低高度角的大气静力学延迟修正是主要的关键技术.比较了3种计算无线电波从空间到地面接收机的大气静力学延迟修正方法:(1)在大气球对称假设下用探空气球资料路径积分得到大气静力学延迟;(2)在大气球对称假设下用NCEP(National Centers for Environmental Prediction)再分析资料得到大气静力学延迟;(3)用Niell的大气静力学映射函数得到大气静力学延迟.与2001年中国地区89个探空气球站资料计算得到的大气静力学映射函数比较结果显示:NCEP再分析资料路径积分的方法在低高度角(5°以下)的精度好于Niell映射函数模型约5倍.     

宁夏河东沙地不同坡度柠条锦鸡儿(Caragana korshinskii)水分利用策略差异

为探讨全球气候变化背景下多元线性混合模型（IsoSource）和贝叶斯混合模型（MixSIAR）解析宁夏河东沙地柠条锦鸡儿（Caragana korshinskii）水分利用策略差异及适用性,利用氢氧稳定同位素技术,结合直接对比法、IsoSource模型和MixSIAR模型对比分析不同坡度（6°、10°、16°和24°）样地柠条锦鸡儿在生长季不同时期对各潜在水源的利用率,并评估两种模型的植物水分溯源效果。结果表明：柠条锦鸡儿对不同土层深度土壤水的利用存在明显的季节性差异,生长季初期,随着坡度的增大,柠条锦鸡儿对中层土壤水的利用率呈现出先增大后减小的趋势;生长季中期,随坡度增大,柠条锦鸡儿主要水分来源由深层土壤转移至浅层土壤;生长季后期,柠条锦鸡儿主要水分来源随着坡度的增大由浅层土壤转移至深层土壤。基于直接对比法的定性判断结果,IsoSource模型和MixSIAR模型计算坡度6°、10°和16°样地柠条锦鸡儿主要水分来源利用率的适用性均较高;而Mix-SIAR模型计算坡度24°样地柠条锦鸡儿主要的水分来源以及其贡献率具有更高的可靠性。Iso-Source模型更适合解析较小坡度（6°和1...